FlyBase Allele Report: Dmel\N[UAS.cSa]

General Information

Symbol

Dmel\N^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Seugnet

FlyBase ID

FBal0048889

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.S}

Key Links

Transgenic product class

wild_type

(Doherty et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Doherty et al., 1996)

Mutagen

in vitro construct

(Doherty et al., 1996, Frise et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-N.S}

Cytology

Description

Expression of N coding region is governed by UASt regulatory sequences.

(Doherty et al., 1996)

N expression is under the control of UAS regulatory sequences.

(Frise et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^dpp.blk1

(Lee et al., 2000)

eye, with Scer\GAL4^sca-109-68

(Li et al., 2003)

macrochaeta, with Scer\GAL4^sca-109-68

(Younger, 2001.2)

microchaeta | ectopic, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

microchaeta | precursor, with Scer\GAL4^410.2

(Seugnet et al., 1997)

microchaeta | precursor, with Scer\GAL4^pnr-MD237

(Seugnet et al., 1997)

neuron, with Scer\GAL4^insc-Mz1407

(Guo et al., 1996)

photoreceptor cell R8, with Scer\GAL4^sca-109-68

(Li et al., 2003)

sense organ, with Scer\GAL4^sca-109-68

(Doherty et al., 1997)

sense organ, with Scer\GAL4^sca-537.4

(Doherty et al., 1997)

thorax | dorsal & macrochaeta, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

tormogen cell, with Scer\GAL4^sca-109-68

(Frise et al., 1996)

tormogen cell | ectopic, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

trichogen cell, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

trichogen cell, with Scer\GAL4^sca-109-68

(Guo et al., 1996)

wing, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

wing & microchaeta, with Scer\GAL4^ptc-559.1

(Doherty et al., 1996)

wing vein | ectopic, with Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Detailed Description

Statement

Reference

When N^Scer\UAS.cSa is driven by Scer\GAL4^pnr-MD237 most of the bristles are lost from the dorsal-most region of the thorax. In addition, occasionally, bristle to socket transformation is observed.

(Chandra et al., 2003)

When N^Scer\UAS.cSa is driven by Scer\GAL4^sca-109-68 R8 photoreceptor cells are sometimes missing. Adult eyes are small and rough.

(Li et al., 2003)

When driven by Scer\GAL4^sca-109-68 bristle defects occur.

(Younger, 2001.2)

The expression of N^Scer\UAS.cSa when driven by Scer\GAL4^dpp.blk1 causes a thickening of wing veins and a reduction of the anterior crossvein.

(Lee et al., 2000)

N^Scer\UAS.cSa causes loss of sense organs and rare hair-to-socket transformations when expressed using Scer\GAL4^sca-537.4 or Scer\GAL4^sca-109-68.

(Doherty et al., 1997)

Scer\GAL4^pnr-MD237-mediated expression can cause loss of bristles. Scer\GAL4^pnr-MD237- or Scer\GAL4^410.2-mediated expression suppresses the development of neural precursors in a shi mutant background.

(Seugnet et al., 1997)

When expression is driven by Scer\GAL4^ptc-559.1, flies show a short row of ectopic hairs along the fourth wing vein on both dorsal and ventral sides of the wing blade, similar to anterior double row or posterior wing margin cells. Occasional wing outgrowth is observed.

(Doherty et al., 1996)

When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4^sca-109-68, a double socket phenotype is produced.

(Frise et al., 1996)

Scer\GAL4¹⁴⁰⁷ induced expression causes mild transformation of neurons to sheath cells. Scer\GAL4^sca-109-68 induced expression causes 20% of the bristles in the notum to form double sockets, or less frequently triple or four sockets.

(Guo et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^UAS.cSa, Scer\GAL4^sca-109-68 has visible phenotype, enhanceable by bib^UAS.cDa, Scer\GAL4^sca-109-68

(Doherty et al., 1997)

N^UAS.cSa, Scer\GAL4^sca-537.4 has visible phenotype, enhanceable by bib^UAS.cDa, Scer\GAL4^sca-537.4

(Doherty et al., 1997)

Enhancer of

Statement

Reference

N^UAS.cSa, Scer\GAL4^sca-109-68 is an enhancer of visible phenotype of Scer\GAL4^sca-109-68, bib^UAS.cDa

(Doherty et al., 1997)

N^UAS.cSa, Scer\GAL4^sca-537.4 is an enhancer of visible phenotype of Scer\GAL4^sca-537.4, bib^UAS.cDa

(Doherty et al., 1997)

Suppressor of

Statement

Reference

N^UAS.cSa, Scer\GAL4^bbg-C96 is a suppressor of visible phenotype of Nsf2^EQ.UAS, Scer\GAL4^bbg-C96

(Stewart et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^UAS.cSa, Scer\GAL4^sca-109-68 has sense organ phenotype, enhanceable by bib^UAS.cDa, Scer\GAL4^sca-109-68

(Doherty et al., 1997)

N^UAS.cSa, Scer\GAL4^sca-537.4 has sense organ phenotype, enhanceable by bib^UAS.cDa, Scer\GAL4^sca-537.4

(Doherty et al., 1997)

NOT Enhanced by

Statement

Reference

N^UAS.cSa, Scer\GAL4^dpp.blk1 has anterior crossvein phenotype, non-enhanceable by sca^UAS.cEa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

N^UAS.cSa, Scer\GAL4^dpp.blk1 has wing vein | ectopic phenotype, non-enhanceable by sca^UAS.cEa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Suppressed by

Statement

Reference

N^UAS.cSa, Scer\GAL4^pnr-MD237 has macrochaeta | ectopic phenotype, suppressible | partially by fred^RNAi.UAS, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

N^UAS.cSa, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, suppressible | partially by fred^RNAi.UAS, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

NOT suppressed by

Statement

Reference

N^UAS.cSa, Scer\GAL4^dpp.blk1 has anterior crossvein phenotype, non-suppressible by sca^UAS.cEa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

N^UAS.cSa, Scer\GAL4^dpp.blk1 has wing vein | ectopic phenotype, non-suppressible by sca^UAS.cEa, Scer\GAL4^dpp.blk1

(Lee et al., 2000)

Enhancer of

Statement

Reference

N^UAS.cSa, Scer\GAL4^sca-109-68 is an enhancer of sense organ phenotype of Scer\GAL4^sca-109-68, bib^UAS.cDa

(Doherty et al., 1997)

N^UAS.cSa, Scer\GAL4^sca-537.4 is an enhancer of sense organ phenotype of Scer\GAL4^sca-537.4, bib^UAS.cDa

(Doherty et al., 1997)

Suppressor of

Statement

Reference

N^UAS.cSa, Scer\GAL4^pnr-MD237 is a suppressor | partially of macrochaeta | ectopic phenotype of Scer\GAL4^pnr-MD237, fred^RNAi.UAS

(Chandra et al., 2003)

N^UAS.cSa, Scer\GAL4^pnr-MD237 is a suppressor | partially of mesothoracic tergum phenotype of Scer\GAL4^pnr-MD237, fred^RNAi.UAS

(Chandra et al., 2003)

N^UAS.cSa, Scer\GAL4^bbg-C96 is a suppressor of wing margin phenotype of Nsf2^EQ.UAS, Scer\GAL4^bbg-C96

(Stewart et al., 2001)

N^UAS.cSa, Scer\GAL4^dpp.blk1 is a suppressor of wing | distal phenotype of Scer\GAL4^dpp.blk1, sca^UAS.cEa

(Lee et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

When fred^{dsRNA.Scer\UAS} and N^Scer\UAS.cSa are both driven by Scer\GAL4^pnr-MD237, a phenotype that is intermediate between the two individual phenotypes.

(Chandra et al., 2003)

N^Scer\UAS.cSa interacts synergistically with bib^Scer\UAS.cDa, producing increased sense organ loss and more frequent hair-to-socket transformations when they are expressed using Scer\GAL4^sca-537.4 or Scer\GAL4^sca-109-68.

(Doherty et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

L. Seugnet, M. Haenlin and P. Simpson.

(Doherty et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^Scer\UAS.cSa

(Ward et al., 2006)

N^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Seugnet

(Doherty et al., 1996)

Secondary FlyBase IDs

References (11)

Report Sections

Open Close