From kcook@XXXX Sat Jul 05 20:28:42 2003
To: flybase-updates@XXXX
Subject: Younger P{GawB} insertions
The following information accompanied stocks donated to the Bloomington
Stock Center by Susan Younger, University of California at San Francisco
(2/01).
I. P{GawB}GII5 is homozygous viable and fertile second chromosome
insertion. In combination with a P{UAS-lacZ} insertion*, staining is seen
in embryonic peritracheal cells and pericardial cells.
(*Note: The specific identities of the P{UAS-lacZ} and P{UAS-Notch}
constructs were not provided.)
II. P{GawB}G11-1 is a homozygous and hemizygous viable and fertile X
chromosome insertion. In combination with a P{UAS-lacZ} insertion,
staining is seen in a subset of the embryonic peripheral nervous system and
uniformly in stage 9 follicle cells.
III. P{GawB}112A is a homozygous and hemizygous viable and fertile X
chromosome insertion generated in the lab of Y.N. Jan. In combination with
a P{UAS-lacZ} insertion, staining is seen in the embryonic epidermis and in
the stalk cells and in follicle cells at the anterior and posterior poles
of the egg chamber.
IV. P{GawB}109C1 is a homozygous and hemizygous viable and fertile X
chromosome insertion generated in the lab of Y.N. Jan. In combination with
a P{UAS-lacZ} insertion, staining is seen in the embryonic epidermis and in
the follicle cells at the anterior and posterior poles of the egg chamber.
V. The following insertions were generated by mobilization of P{GawB}109C1
in the lab of Y.N. Jan.
A. P{GawB}109-28
1. A homozygous viable and fertile second chromosome insertion.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in the
follicle cells of the dorsal appendage.
B. P{GawB}109-30
1. This insertion is carried on the second chromosome.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in the
embryonic dorsal midline and peripheral nervous system, the larval ventral
nerve cord and segmentally in the nervous system, and in stalk cells,
follicle cells at the poles of the egg chamber and the precursors to the
follicle cells in the germarium.
3. In combination with P{UAS-N}, it is larval lethal.
4. In combination with P{UAS-Cdc42.L}, it is larval lethal.
C. P{GawB}109-39
1. This insertion is carried on the third chromosome.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in
blastoderm stripes, and in stage 2 follicle cells.
1. In combination with P{UAS-N}, it is larval lethal.
2. In combination with P{UAS-Cdc42.L}, it is larval lethal.
3. In combination with P{UAS-Rac1.L}, it is larval lethal.
D. P{GawB}109-53
1. This insertion is carried on the third chromosome.
2. In combination with a P{UAS-lacZ} insertion, general staining is seen in
embryos. In ovaries, staining is seen in stalk cells and terminal filament
cells.
1. In combination with P{UAS-numb.W}, it is embryonic-larval lethal.
2. In combination with P{UAS-N}, it is larval lethal.
3. In combination with P{UAS-Cdc42.L}, it is larval lethal.
E. P{GawB}sca109-68 (FBti0004036)
1. A homozygous lethal, second chromosome insertion.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in
proneural cells of the wing imaginal disk and in stage 6 follicle cells.
3. In combination with P{UAS-numb.W}, it causes bristle defects.
4. In combination with P{UAS-N}, it causes bristle defects.
5. In combination with P{UAS-Cdc42.L}, it is larval lethal.
6. In combination with P{UAS-Rac1.L},it causes bristle defects.
F. P{GawB}109-69
1. This insertion is carried on the second chromosome.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in
embryonic dorsal epidermis and in parts of the peripheral nervous system,
in the larval wing imaginal disk and parts of the brain, and in stage 8
follicle cells.
3. In combination with P{UAS-Cdc42.L}, it is larval lethal.
4. In combination with P{UAS-Rac1.L}, it causes rough eyes.
G. P{GawB}109-79
1. A homozygous viable and fertile second chromosome insertion.
2. In combination with a P{UAS-lacZ} insertion, staining is not seen in
embryos, but strong staining is seen in stage 2-3 follicle cells.
5. In combination with P{UAS-numb.W}, it is embryonic-larval lethal.
6. In combination with P{UAS-N}, it is larval lethal.
7. In combination with P{UAS-Cdc42.L}, it is larval lethal.
8. In combination with P{UAS-Rac1.L},it is embryonic-larval lethal.
VI. The following insertions were generated in the lab of Gerhard Technau.
A. P{GawB}1158x
1. A homozygous and hemizygous viable and fertile X chromosome insertion.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in
embryonic ventral muscles and a subset of the CNS.
B. P{GawB}60IIA
1. A homozygous viable and fertile second chromosome insertion.
2. In combination with a P{UAS-lacZ} insertion, staining is seen in the
nervous system.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX
