FlyBase Allele Report: Dmel\fred[RNAi.UAS]

General Information

Symbol

Dmel\fred^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0150494

Feature type

allele

Associated gene

Dmel\fred

Associated Insertion(s)

Carried in Construct

P{UAS-fred.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Chandra et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Chandra et al., 2003)

Mutagen

in vitro construct

(Chandra et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-fred.RNAi}

Cytology

Description

UASt sequences drive expression of two copies of nucleotides 3688-4326 of the fred gene in an inverted repeat.

(Chandra et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

fred

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

dorsal mesothoracic disc & sensory mother cell | dorsal | ectopic, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

dorsal mesothoracic disc & sensory mother cell | ectopic, with Scer\GAL4^ap-md544

(Chandra et al., 2003)

eye, with Scer\GAL4^GMR.PF

(Chandra et al., 2003)

macrochaeta, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

mesothoracic tergum, with Scer\GAL4^Eq

(Chandra et al., 2003)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Fetting et al., 2009)

scutellum, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

Detailed Description

Statement

Reference

Many ommatidia in animals expressing fred^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type. The mutant s.d. does not become statistically distinct from wild type until row 7 in the third larval instar eye disc, approximately 2-3 rows after the initiation of rotation (the time at which the anterior and posterior cone cells join the ommatidial precursor). The s.d. is still significantly greater in the mutants than in wild type at row 15, when rotation is complete in wild type.

(Fetting et al., 2009)

When fred^{dsRNA.Scer\UAS} is driven by Scer\GAL4^pnr-MD237 larvae develop into adults that display a range of phenotypes, including loss of epithelium resulting in a smaller notum and scutellum and loss or duplication of sensory bristles. The phenotypes are more severe is raised at 29^oC. Ectopic sensory organ precursors (SOPs) are seen in the dorsal-most region of the wing disc. This is associated with an increase in cell death in the region. When fred^{dsRNA.Scer\UAS} is driven by Scer\GAL4^Eq1 larvae develop into adults that display a range of phenotypes, including loss of epithelium, resulting in a pinched appearance of the nota. When fred^{dsRNA.Scer\UAS} is driven by Scer\GAL4^ap-md544 ectopic sensory organ precursors (SOPs) are seen in the wing disc. When HLHm7^{Scer\UAS.cdCa} is added fred^{dsRNA.Scer\UAS}, Scer\GAL4^pnr-MD237 animals, no effect is seen on the HLHm7^{Scer\UAS.cdCa} phenotype. When fred^{dsRNA.Scer\UAS} is driven by Scer\GAL4^GMR.PF, a rough eye phenotype is seen, with missing or duplicated bristles or fused ommatidia.

(Chandra et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has visible phenotype, non-enhanceable by Su(H)[+]/Su(H)²

(Chandra et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has increased cell death phenotype, suppressible by Scer\GAL4^pnr-MD237/Su(H)^UAS.cKa

(Chandra et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has visible phenotype, non-suppressible by Su(H)[+]/Su(H)²

(Chandra et al., 2003)

NOT Enhancer of

Statement

Reference

fred^RNAi.UAS, Scer\GAL4^pnr-MD237 is a non-enhancer of visible phenotype of E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

NOT Suppressor of

Statement

Reference

fred^RNAi.UAS, Scer\GAL4^pnr-MD237 is a non-suppressor of visible phenotype of E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, fred^RNAi.UAS has eye phenotype, enhanceable by H^UAS.cMa, Scer\GAL4^GMR.PF

(Chandra et al., 2003)

Scer\GAL4^GMR.PF, fred^RNAi.UAS has interommatidial bristle phenotype, enhanceable by H^UAS.cMa, Scer\GAL4^GMR.PF

(Chandra et al., 2003)

Scer\GAL4^GMR.PF, fred^RNAi.UAS has ommatidium phenotype, enhanceable by H^UAS.cMa, Scer\GAL4^GMR.PF

(Chandra et al., 2003)

Scer\GAL4^GMR.PF, fred^RNAi.UAS has eye phenotype, enhanceable by Gap1[+]/RasGAP1^B2

(Chandra et al., 2003)

Scer\GAL4^GMR.PF, fred^RNAi.UAS has eye phenotype, enhanceable by Su(H)[+]/Su(H)²

(Chandra et al., 2003)

Scer\GAL4^GMR.PF, fred^RNAi.UAS has eye phenotype, enhanceable by ed[+]/ed^2B8

(Chandra et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, fred^RNAi.UAS has eye phenotype, suppressible by pnt[+]/pnt^Δ88

(Chandra et al., 2003)

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has mesothoracic tergum phenotype, suppressible by Df(1)sc10-1

(Chandra et al., 2003)

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has macrochaeta | ectopic phenotype, suppressible by Df(1)sc10-1, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has macrochaeta | ectopic phenotype, suppressible | partially by N^UAS.cSa, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

Scer\GAL4^pnr-MD237, fred^RNAi.UAS has mesothoracic tergum phenotype, suppressible | partially by N^UAS.cSa, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

NOT Enhancer of

Statement

Reference

Scer\GAL4^pnr-MD237/fred^RNAi.UAS is a non-enhancer of mesothoracic tergum | medial phenotype of Scer\GAL4^pnr-MD237, Su(H)^UAS.cKa

(Chandra et al., 2003)

Suppressor of

Statement

Reference

fred^RNAi.UAS, Scer\GAL4^pnr-MD237 is a suppressor | partially of mesothoracic tergum phenotype of N^UAS.cSa, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

fred^RNAi.UAS, Scer\GAL4^pnr-MD237 is a suppressor | partially of macrochaeta | ectopic phenotype of N^UAS.cSa, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^pnr-MD237/fred^RNAi.UAS is a non-suppressor of mesothoracic tergum | medial phenotype of Scer\GAL4^pnr-MD237, Su(H)^UAS.cKa

(Chandra et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

When Df(1)sc10-1 is added to fred^{dsRNA.Scer\UAS}, Scer\GAL4^pnr-MD237, animals show no bristles and have a near wild-type notum morphology. When fred^{dsRNA.Scer\UAS} and N^Scer\UAS.cSa are both driven by Scer\GAL4^pnr-MD237, a phenotype that is intermediate between the two individual phenotypes. The addition of fred^{dsRNA.Scer\UAS} to Su(H)^Scer\UAS.cKa, Scer\GAL4^pnr-MD237 animals has no effect on the Su(H)^Scer\UAS.cKa phenotype. The cell death phenotype seen in Su(H)^Scer\UAS.cKa animals is however, alleviated by Su(H)^Scer\UAS.cKa expression. The addition of Su(H)²/+ to fred^{dsRNA.Scer\UAS}, Scer\GAL4^pnr-MD237 animals has no detectable effect on the fred^{dsRNA.Scer\UAS} phenotype. However when Su(H)²/+ is added to fred^{dsRNA.Scer\UAS} driven by Scer\GAL4^GMR.PF, an enhancement of the rough eye phenotype is seen. The addition of heterozygous ed^2B8 enhances the rough eye phenotype seen in fred^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF animals. The addition of heterozygous Gap1^B2 moderately enhances the rough eye phenotype seen in fred^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF animals. However only 30% of animals exhibited increased roughness. The addition of heterozygous pnt^Δ88 suppresses the rough eye phenotype seen in fred^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF animals. However only 40% of animals exhibited decreased roughness. The addition of fred^{dsRNA.Scer\UAS} enhances the effect of H^Scer\UAS.cNa (driven by Scer\GAL4^GMR.PF) on eye morphology.

(Chandra et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fred^RNAi.UAS

fred^{dsRNA.Scer\UAS}

fred^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close