XX flies homozygous for tra^unspecified develop as 'pseudo-males' and are essentially indistinguishable from males in morphology and behavior but are sterile.

Heterozygous adults have normal sexual differentiation.

XX dsf^- tra^- animals show the ventral abdominal muscle innervation phenotype, while their XX dsf⁺ tra^- siblings do not, indicating that dsf acts downstream of tra.

Sxl^M1/X ; tra^unspecified/tra^unspecified and Sxl^M4/X ; tra^unspecified/tra^unspecified larvae have gonads that are indistinguishable from XX ; tra^unspecified/tra^unspecified larvae. Sxl^M1/X ; tra^unspecified/tra^unspecified and Sxl^M4/X ; tra^unspecified/tra^unspecified adult pseudomales often have degenerating cells in the testes. Germ cells in these flies can be either oogenic or spermatogenic (a single animal contains only oogenic or spermatogenic testes, not both).

XX homozygotes have male internal genitalia.

Chromosomal females (XX) homozygous for tra^unspecified are transformed into "pseudomales". They are sterile even in the presence of a Y chromosome. The gonads form testes which are generally non-gametogenic, containing degenerated germ cells and debris or gonial cells whose sex cannot be determined. A variable proportion are gametogenic, containing either oogenic or spermatogenic stages of germ cell development (never both in the same gonad). In these cases oogenesis is arrested anywhere between stages S3 and S10A, and spermatogenesis is frequently arrested at the spermatocyte stage, although some later stages of spermatogenic differentiation are seen. Triploid intersexes with a chromosome ratio of 2X:3A are transformed to pseudomales in animals in which all three copies of tra are mutant. The gonads form well developed and nicely coiled testes, which are mostly filled with spermatogenic germ cells which were usually arrested at the spermatocyte stage.

Doa⁷, tra^unspecified has abnormal sex-determination | female phenotype

Doa⁷/Doa^unspecified, tra^unspecified has abnormal sex-determination | dominant | female phenotype

Doa^unspecified/Doa^Dem, tra^unspecified has abnormal sex-determination | dominant | female phenotype

Doa^Dem, tra^unspecified has abnormal sex-determination | female phenotype

Doa^unspecified, tra^unspecified has abnormal sex-determination | female phenotype

Doa⁷, tra^unspecified has leg | female phenotype

Doa⁷/Doa^unspecified, tra^unspecified has leg | female phenotype

Doa^unspecified/Doa^Dem, tra^unspecified has female genitalia phenotype

Doa^unspecified/Doa^Dem, tra^unspecified has leg | female phenotype

Doa^unspecified/Doa^Dem, tra^unspecified has tergite | female phenotype

Doa^Dem, tra^unspecified has female genitalia phenotype

Doa^Dem, tra^unspecified has leg | female phenotype

Doa^Dem, tra^unspecified has tergite | female phenotype

Doa^unspecified, tra^unspecified has female genitalia phenotype

Doa^unspecified, tra^unspecified has leg | female phenotype

Doa^unspecified, tra^unspecified has tergite | female phenotype