D.melanogaster flies carrying Ccap\trahs.PP and raised at 25oC can be divided into three classes according to the severity of defects seen in XY flies. In the weak class, the majority of XX and XY flies have no obvious sex transformation, having normal external morphology and being fully viable and fertile. However 2% of XY flies in the weak class have male intersexual phenotypes, with reduced abdomen pigmentation, incomplete sex-comb development, and variable feminisation of the external genitalia. In the intermediate class, XY animals have a variety of phenotypes, ranging from male to female-like external morphology. 30% of the animals in this class are intersexes. Finally, in the strong class, XY animals are always strongly feminised; they have female-like reduced abdominal pigmentation, no sex combs on the forelegs and the external genitalia is made up of both male and female tissues. These animals have a body size similar to that of wild-type males. D.melanogaster XY flies carrying Ccap\trahs.PP and exposed to a daily heat shock regimen are strongly transformed towards femaleness, with female-like abdominal pigmentation, reduced or absent sex combs and apparently normal female-like external genitalia. These pseudofemales are sterile. D.melanogaster XX flies carrying Ccap\trahs.PP do not show sexually altered somatic tissues either at 25oC, or after heat shock treatment.
Ccap\trahs.PP is a suppressor of abnormal sex-determination | female phenotype of traunspecified
Ccap\trahs.PP is a non-suppressor of abnormal sex-determination | female phenotype of tra2B/Df(2R)trix
Expression of Ccap\trahs.PP using heat shock in XX ; traunspecified animals results in the development of rudimentary or even almost-normal ovaries. However, these animals fail to lay eggs. XY ; Ccap\trahs.PP traunspecified animals are transformed into somatic females at 25oC (as occurs in XY ; Ccap\trahs.PP animals). However, these individuals have either malformed testes, or lack internal genitalia. The mutant phenotype of XX ; tra2B/Df(2R)trix animals is not rescued by Ccap\trahs.PP.
Assayed in D.melanogaster.