FlyBase Allele Report: Dmel\dhd[J5]

General Information

Symbol

Dmel\dhd^J5

Species

D. melanogaster

Name

FlyBase ID

FBal0039237

Feature type

allele

Associated gene

Dmel\dhd

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Salz et al., 1994)

Mutagen

Delta2-3 transposase

(Salz et al., 1994)

Progenitor genotype

dhd^P8

(Salz et al., 1994)

Caused by aberration

Df(1)J5

Cytology

Description

Deletion of approximately 1.5kb which does not affect the function of neighboring genes.

(Salz et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\GPR37^UAS.cYa

(Umeda-Kameyama et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

dhd^J5 homozygous and dhd^J5/Df(1)JC70 transheterozygous females are sterile, as all eggs fail to hatch. After fertilization of dhd^J5-homozygous female-derived eggs, the male pronucleus chromatin fails to decondense (i.e. maintains a needle-shape), remains connected to the acrosome and, although still keeping the sperm aster in its vicinity, the release of the paternal centrioles is delayed, as compared to controls; the male pronucleus does not participate in development (i.e. does not replicate), leading to an effectively haploid syncytial embryo. The female pronucleus often fails to migrate towards the male pronucleus.

dhd^J5/Df(1)JC70 transheterozygous are also female sterile; after fertilization of their eggs, the male pronucleus chromatin fails to decondense (i.e. maintains a needle-shape).

(Tirmarche et al., 2016)

gnu³⁰⁵, plu³ and png⁵ homozygous females lay eggs with a 'giant nuclei' phenotype due to premature initiation of DNA synthesis, dhd^J5 cannot suppress the 'giant nuclei' phenotype in double mutants.

(Pellicena-Palle et al., 1997)

Eggs from mutant mothers fail to complete meiosis. In 90% of embryos there is no sign of development and no evidence of a female pronucleus. Normal polar body structures are only rarely observed. Most common phenotype is that eggs contain a structure that resembles a nucleus arrested in anaphase. In the 10% of embryos that initiate development, there are defects in preblastoderm nuclear divisions. Divisions are not synchronous. Not all nuclei migrate to the periphery. 5% of embryos develop far enough to secret cuticle. Head involution fails during gastrulation. In embryos where head involution is partial, the most prominent defects are shortening of the lateralgraten and fusion of the ventral arms of the cephalopharyngeal skeleton.

(Salz et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

dhd^J5 has female sterile phenotype, non-suppressible by Df(2L)ΔMst35B/Df(2L)ΔMst35B

(Tirmarche et al., 2016)

dhd^J5 has lethal - all die during embryonic stage | maternal effect phenotype, non-suppressible by Df(2L)ΔMst35B/Df(2L)ΔMst35B

(Tirmarche et al., 2016)

Enhancer of

Statement

Reference

dhd^J5, dhd[+], Scer\GAL4^elav.PLu is an enhancer of abnormal locomotor behavior phenotype of Hsap\GPR37^UAS.cYa

(Umeda-Kameyama et al., 2007)

Other

Statement

Reference

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR}, dhd^+t4.3, dhd^J5 has female semi-sterile phenotype

(Torres-Campana et al., 2022)

Scer\GAL4^{VP16.nanos.UTR}, Snr1^HMS00363, dhd^+t4.3, dhd^J5 has female sterile phenotype

(Torres-Campana et al., 2022)

Scer\GAL4^{VP16.nanos.UTR}, dhd^+t4.3, dhd^J5, mod(mdg4)^HMS00849 has female semi-sterile phenotype

(Torres-Campana et al., 2022)

Scer\GAL4^{VP16.nanos.UTR}, Sin3A^HMS00359, dhd^+t4.3, dhd^J5 has female semi-sterile phenotype

(Torres-Campana et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

dhd^J5 has male pronucleus | maternal effect phenotype, suppressible by Df(2L)ΔMst35B/Df(2L)ΔMst35B

(Tirmarche et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The female sterility of dhd^J5 homozygotes is not rescued by fertilization with Df(2L)ΔMst35B homozygous sperm. The male pronucleus chromatin defects observed upon fertilization of dhd^J5 homozygous-derived eggs is partially suppressed by fertilization with Df(2L)ΔMst35B homozygous sperm.

(Tirmarche et al., 2016)

Xenogenetic Interactions

Statement

Reference

The reduction in climbing ability seen in 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu is made more severe by the addition of dhd^J5/+.

(Umeda-Kameyama et al., 2007)

Complementation and Rescue Data

Rescued by

dhd^J5 is rescued by dhd^+t4.3

(Torres-Campana et al., 2022)

dhd^J5 is rescued by dhd^FD

(Torres-Campana et al., 2022)

dhd^J5 is rescued by dhd^+t4.8

(Tirmarche et al., 2016)

dhd^J5 is rescued by dhd^+t4.3

(Tirmarche et al., 2016)

dhd^J5 is rescued by dhd^hs.PP

(Pellicena-Palle et al., 1997)

Not rescued by

dhd^J5 is not rescued by dhd^ΔDRE

(Torres-Campana et al., 2022)

dhd^J5 is not rescued by dhd^tC34S

(Tirmarche et al., 2016)

dhd^J5 is not rescued by dhd^C31S.C34S.hs

(Pellicena-Palle et al., 1997)

Comments

The female sterility of dhd^J5 homozygotes is rescued by dhd^+t4.8 or dhd^+t4.3, but not dhd^tC34S. The male pronucleus chromatin defects observed upon fertilization of eggs laid by dhd^J5 homozygous females is rescued by dhd^+t4.3, but not dhd^tC34S.

(Tirmarche et al., 2016)

Female sterility can be rescued by dhd^hs.PP, but not by dhd^C31S.C34S.hs.

(Pellicena-Palle et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mutant phenotype is not rescuable by paternally contributed wild type allele.

(Salz et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dhd^J5

(Torres-Campana et al., 2022, Petrova et al., 2018, Tirmarche et al., 2016, Umeda-Kameyama et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (8)

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