FlyBase Allele Report: Dmel\tor::tub[t4021t]

General Information

Symbol

Dmel\tor::tub^t4021t

Species

D. melanogaster

Name

FlyBase ID

FBal0035801

Feature type

allele

Associated gene

tor::tub

Associated Insertion(s)

Carried in Construct

P{tor⁴⁰²¹tube}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Galindo et al., 1995, Großhans et al., 1994)

Progenitor genotype

tor^13D

(Galindo et al., 1995)

Carried in construct

P{tor⁴⁰²¹tube}

Cytology

Description

Construct: Residues 1-455 of tor^13D, including the extracellular and transmembrane domain, fused to the entire ORF of tub.

(Galindo et al., 1995)

First 455 amino acids of tor, containing the signal peptide, extracellular domain with a single amino acid replacement and transmembrane peptide, are fused to amino acids 2-462 of tub.

(Großhans et al., 1994)

Allele components

Component

Use(s)

Encoded product / tool

tor::tub

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

When injected into embryos from snk¹/snk² mothers, filzkorper and ventral denticles show 84% rescue. tor::tub^WT and tor::tub^t4021t both induce filzkorper and denticle belts, but tor::tub^t4021t has a much higher activity.

(Großhans et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

tor::tub^t4021t is a non-suppressor of lethal | embryonic stage phenotype of snk^unspecified

(Charatsi et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

tor::tub^t4021t is a suppressor of embryonic epidermis | ventral phenotype of Myd88^kra-1

(Charatsi et al., 2003)

tor::tub^t4021t is a suppressor of ventral denticle belt | ventral phenotype of tub^unspecified

(Galindo et al., 1995)

tor::tub^t4021t is a suppressor of filzkorper phenotype of tub^unspecified

(Galindo et al., 1995)

tor::tub^t4021t is a suppressor of embryonic epidermis | ventral phenotype of tub^unspecified

(Galindo et al., 1995)

tor::tub^t4021t is a suppressor of ventral denticle belt | ventral phenotype of gd^unspecified

(Galindo et al., 1995)

tor::tub^t4021t is a suppressor of filzkorper phenotype of gd^unspecified

(Galindo et al., 1995)

tor::tub^t4021t is a suppressor of embryonic epidermis | ventral phenotype of gd^unspecified

(Galindo et al., 1995)

NOT Suppressor of

Statement

Reference

tor::tub^t4021t is a non-suppressor of embryo | ventral phenotype of pll⁷

(Galindo et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

When tor::tub^t4021t is injected into embryos laid by snk^unspecified mothers, significant rescue of the dorsalised phenotype is seen. Some ventrolateral pattern elements are seen, such as ventral denticle belts and Filzkorper, which are never seen in Myd88^kra-1 mutant embryos.

(Charatsi et al., 2003)

Injection of RNA into wild type, gd and tub null embryos rescues ventral fate, filzkorper and ventral denticle belts are evident. Injection into pll⁷ embryos effects no ventral rescue.

(Galindo et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "tor⁴⁰²¹tub" and used in transient expression assays in embryos.

(Großhans et al., 1999)

Transcribed in vitro and injected into Myd88^kra-1 or snk^unspecified embryos to study interaction.

(Charatsi et al., 2003)

Ventralising gain of function allele of tub.

(Großhans et al., 1994)

Carried in plasmid "tor⁴⁰²¹-tub", used to generate RNA for microinjection studies.

(Galindo et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tor::tub^13D

tor::tub^t4021t

Name Synonyms

Secondary FlyBase IDs

FBal0044429

References (4)

Report Sections

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