FlyBase Allele Report: Dmel\per[SG.lacZ]

General Information

Symbol

Dmel\per^SG.lacZ

Species

D. melanogaster

Name

FlyBase ID

FBal0035381

Feature type

allele

Associated gene

Dmel\per

Associated Insertion(s)

Carried in Construct

P{per-lacZ.SG}

Also Known As

per-lacZ

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Stanewsky et al., 1997, Helfrich-Forster, 1995, Vosshall et al., 1994, Huang et al., 1993, Liu et al., 1992, Liu et al., 1991, Zwiebel et al., 1991, Liu et al., 1988)

Progenitor genotype

Carried in construct

P{per-lacZ.SG}

Cytology

Description

A genomic per fragment containing 4.2kb 5' flanking sequences and part of the coding region (encoding the N-terminal 638 amino acids) is fused in frame to lacZ.

(Stanewsky et al., 1997)

4kb fragment of per 5' flanking sequences upstream of the transcription start site, the same extent as in per^+t13.2, and genomic sequences up to that encoding approximately the first 630 amino acids, within the fifth exon, are fused to lacZ.

(Liu et al., 1988)

Allele components

Component

Use(s)

Regulatory region(s)

per

Encoded product / tool

per

Tagged with

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval DN1 neuron

(Kaneko et al., 1997)

larval DN2 neuron

(Kaneko et al., 1997)

larval DN3 neuron

(Kaneko et al., 1997)

larval LN period neuron

(Kaneko et al., 1997)

ring gland

(Kaneko et al., 1997)

mouth hook

Comment: Expression is observed in tissue surrounding the mouth hook.

(Kaneko et al., 1997)

larval ventral nerve cord | subset

(Kaneko et al., 1997)

embryonic/larval brain | ventral

(Kaneko et al., 1997)

Additional Information

Statement

Reference

Expression was first seen in 12-14hr old first instar larvae. Expression was detected by X-gal histochemistry in DN2, but not DN1, in L2 larvae. Throughout larval development, expression is seen in cells which do not cycle per found in the ventral region of the brain.

(Kaneko et al., 1997)

Marker for

Reflects expression of

per

(Kaneko et al., 1997)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm

(Stanewsky et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

The phase of the morning activity peak of flies carrying per^{SG.T:Ecol\lacZ} in a per⁺ background is similar to that of wild-type controls, while the phase of the evening activity peak is significantly different, being earlier than wild-type controls, when flies are kept under 12 hour light/12 hour dark cycling conditions. The free-running locomotor activity periods of flies carrying per^{SG.T:Ecol\lacZ} kept under DD conditions are significantly shorter than wild-type controls, and only 70-80% of the flies are rhythmic under these conditions.

(Stanewsky et al., 1997)

Has little or no effect on circadian locomotor activity rhythms in a per⁺ background.

(Zwiebel et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

per^SG.lacZ fails to rescue per⁰¹

(Stanewsky et al., 1997)

per^SG.lacZ fails to rescue per⁰¹

(Zwiebel et al., 1991)

Comments

Fails to restore the missing locomotor activity rhythms of per⁰¹ flies.

(Zwiebel et al., 1991)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

per^{SG.T:Ecol\lacZ} RNA and protein cycling in per⁺ and per⁰¹ flies has been studied.

(Zwiebel et al., 1991)

Used, in comparison with Ecol\lacZ^per.NG and per^1.T:Ivir\HA1 to demonstrate homotypic interactions of per protein in vivo.

(Huang et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

per-SG

(Price et al., 1998)

per^{SG.T:Ecol\lacZ}

per^SG.T:lacZ

per^SG.lacZ

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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