FB2024_03 , released June 25, 2024
Recombinant Construct: P{per-lacZ.SG}
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General Information
Symbol
P{per-lacZ.SG}
FlyBase ID
FBtp0000629
Feature type
transgenic_transposable_element
Component Allele(s)
perSG.lacZ
Also Known As
per-lacZ
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele perSG.lacZ
Transgenic product class
Component
Use(s)
Regulatory region(s)
per
Encoded product / tool
per
Tagged with
lacZ
Description

A genomic per fragment containing 4.2kb 5' flanking sequences and part of the coding region (encoding the N-terminal 638 amino acids) is fused in frame to lacZ.

(Stanewsky et al., 1997)

4kb fragment of per 5' flanking sequences upstream of the transcription start site, the same extent as in per+t13.2, and genomic sequences up to that encoding approximately the first 630 amino acids, within the fifth exon, are fused to lacZ.

(Liu et al., 1988)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
larval DN1 neuron
(Kaneko et al., 1997)
larval DN2 neuron
(Kaneko et al., 1997)
larval DN3 neuron
(Kaneko et al., 1997)
larval LN period neuron
(Kaneko et al., 1997)
ring gland
(Kaneko et al., 1997)
mouth hook

Comment: Expression is observed in tissue surrounding the mouth hook.

(Kaneko et al., 1997)
larval ventral nerve cord | subset
(Kaneko et al., 1997)
embryonic/larval brain | ventral
(Kaneko et al., 1997)
Additional Information
Statement
Reference

Expression was first seen in 12-14hr old first instar larvae. Expression was detected by X-gal histochemistry in DN2, but not DN1, in L2 larvae. Throughout larval development, expression is seen in cells which do not cycle per found in the ventral region of the brain.

(Kaneko et al., 1997)
 
Marker for
Reflects expression of
per
(Kaneko et al., 1997)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{per-lacZ.SG}
(Liu et al., 1988)
descendant(s)
Comments

report comment: In addition to 5\' regulatory sequences, the first 630 amino acids of per protein fused to lacZ coding region.

(Liu et al., 1988)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P{per-lacZ.630}
(FlyBase, 1996-)
P{per-lacZ.SG}
(FlyBase, 1996-)
SG
(Kaneko et al., 1997)
SG-lacZ
(Stanewsky et al., 2002)
SG3
(Cyran et al., 2005)
per-lacZ
(Matsumoto et al., 1999, Kaneko et al., 1997)
Secondary FlyBase IDs
    References (16)