FB2024_03 , released June 25, 2024
Allele: Dmel\per+t13.2
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General Information
Symbol
Dmel\per+t13.2
Species
D. melanogaster
Name
FlyBase ID
FBal0035359
Feature type
allele
Associated gene
Dmel\per
Associated Insertion(s)
Carried in Construct
P{per13.2.cp}
P{per13.2.W8}
P{per13.2}
Key Links
Genomic Maps

Transgenic product class
genomic_DNA
(Citri et al., 1987)
wild_type
(Citri et al., 1987)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Citri et al., 1987)
wild_type
(Citri et al., 1987)
Mutagen
in vitro construct
(Cooper et al., 1994, Rutila et al., 1992, Liu et al., 1991, Wheeler et al., 1991, Dushay et al., 1990, Zerr et al., 1990, Petersen et al., 1988, Citri et al., 1987)
Progenitor genotype
Carried in construct
P{per13.2.cp}
P{per13.2.W8}
P{per13.2}
Cytology
Description

7.5kb per exons and introns and 3.5kb 5' and 2.0kb 3' flanking sequence.

(Cooper et al., 1994)

13.2kb genomic BamHI-EcoRI fragment including per transcription unit.

(Citri et al., 1987)
Allele components
Component
Use(s)
Regulatory region(s)
per
Encoded product / tool
per
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
rescue_region
X:2,681,587..2,694,699 [+]
Linked to:
BamHI-EcoRI restriction fragment
Comment:

Position of restriction fragment on reference sequence inferred by FlyBase curator.

(Citri et al., 1987)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The circadian mating rhythm of D.melanogaster kept in constant darkness dark is altered by the presence of per+t13.2 : Peak mating activity occurs earlier (circadian time (CT) 6hours) than in wild-type flies (CT 9hours) and is maintained for the next 6 hours, rather than dropping off to a slightly lower plateau as in wild-type. There is a secondary peak at CT 0hours not seen in wild-type. In a mixed population, progeny resulting from mating between per+t13.2 carrying flies and Dpse\perper.PT carrying flies are less common than those between flies carrying the same transgene. (This data is for a population in which males have had there wings removed to eliminate mating song difference as a variable, kept in constant darkness.) 90 minute matings at particular points in the circadian cycle show that this effect is most pronounced at circadian time 12 hours, when the ratio is approximately 5:1 , and is greater than 2:1 at other circadian time points.

      (Tauber et al., 2003)

      Circadian rhythm is the same as wild type.

      (Hamblen-Coyle et al., 1992)

      Male D.melanogaster per01 flies carrying per+t13.2 produce courtship songs with a period of about 55 seconds, characteristic of D.melanogaster.

      (Wheeler et al., 1991)

      per01 flies carrying two copies of per+t13.2 are rhythmic. per+ flies carrying per+t13.2 have slightly shorter free running periods than wild-type.

      (Petersen et al., 1988)

      Rhythmicity is as strong as for flies with endogenous wild type per DNA.

      (Citri et al., 1987)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      per+t13.2 rescues per01

      (Beaver and Giebultowicz, 2004)

      per+t13.2 rescues per01

      (Beaver et al., 2002)

      per+t13.2 rescues per01

      (Peixoto et al., 1998)

      per+t13.2 rescues per01

      (Cooper et al., 1994)

      per+t13.2 rescues per01

      (Rutila et al., 1992)

      per+t13.2 rescues per01

      (Liu et al., 1991)

      per+t13.2 rescues per01

      (Citri et al., 1987)
      Partially rescues

      per+t13.2 partially rescues perSLIH

      (Hamblen et al., 1998)

      per+t13.2 partially rescues per01

      (Gailey et al., 1991)
      Comments

      The extension of time spent copulating seen in per01 hemizygous males mated with wild type females is suppressed by per+t13.2.

      (Beaver and Giebultowicz, 2004)

      per+t13.2 suppresses the reduced fertility of per01 mutant flies, and the reduction of sperm numbers in seminal vesicles of two day old per01 mutant males.

      (Beaver et al., 2002)

      Rescues the arrhythmicity of per01 flies.

      (Cooper et al., 1994)

      Transgenic per01 flies are almost always rhythmic with near normal periodicities.

      (Gailey et al., 1991)

      Rescues the arrhythmic phenotype of per01 flies well, with per01 per+t13.2 flies having an average period only marginally longer than wild-type.

      (Liu et al., 1991)

      Rescues of the arrhythmicity of per01 flies as assayed by actogram.

      (Citri et al., 1987)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      per expression, with respect to behavioural circadian rhythms, is not fully dosage compensated.

      (Cooper et al., 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      per+t13.2
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0035360
      References (25)