FlyBase Allele Report: Dmel\grp[06034]

(Jaklevic and Su, 2004, Ji et al., 2004, Peters et al., 2002, Spradling et al., 1999, Fogarty et al., 1997, Sibon et al., 1997, Sullivan et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | embryonic stage

(Fogarty et al., 1997)

abnormal mitotic cell cycle

(Stumpff et al., 2005, Fogarty et al., 1997)

abnormal mitotic cell cycle | embryonic stage

(McCleland et al., 2009)

female sterile

(Su et al., 1999)

female sterile | recessive

(Spradling et al., 1999)

fertile

(Bi et al., 2005)

increased occurrence of cell division | embryonic stage 4

(Seller et al., 2019)

lethal | maternal effect | recessive

(Sullivan et al., 1993)

lethal | recessive

(Su et al., 1998)

lethal - all die during embryonic stage | maternal effect

(Rickmyre et al., 2007, Fogarty et al., 1997, Sibon et al., 1997)

lethal - all die during embryonic stage | maternal effect (with Df(2L)H20)

(Rickmyre et al., 2007)

lethal - all die during embryonic stage | maternal effect (with grp²⁰⁹)

(LaRocque et al., 2007)

lethal - all die during embryonic stage | maternal effect (with grp^Z5170)

(LaRocque et al., 2007)

radiation sensitive

(Jaklevic et al., 2006)

some die during embryonic stage | maternal effect

(Rickmyre et al., 2007, Fogarty et al., 1997, Sibon et al., 1997)

some die during embryonic stage | maternal effect (with Df(2L)H20)

(Rickmyre et al., 2007)

some die during embryonic stage | maternal effect (with grp²⁰⁹)

(LaRocque et al., 2007)

some die during embryonic stage | maternal effect (with grp^Z5170)

(LaRocque et al., 2007)

viable

(Jaklevic et al., 2006, Bi et al., 2005, Su et al., 1999)

Phenotype Manifest In

centrosome | embryonic stage 4

(Stumpff et al., 2005)

embryo | embryonic cycle 11

(Yu et al., 2000)

embryo | embryonic cycle 11 | maternal effect

(Sullivan et al., 1993)

embryo | embryonic cycle 12 | maternal effect

(Sullivan et al., 1993)

embryo | embryonic cycle 13

(Yu et al., 2000)

embryo | embryonic cycle 13 | maternal effect

(Sullivan et al., 1993)

embryo | embryonic cycle 14 | maternal effect

(Sullivan et al., 1993)

mitosis & nuclear chromosome

(Yu et al., 2000)

mitotic cell cycle | embryonic stage

(Su et al., 1999)

mitotic domain 1 | embryonic cycle 12

(Yu et al., 2000)

mitotic metaphase | maternal effect

(Fogarty et al., 1997)

mitotic telophase | maternal effect

(Fogarty et al., 1997)

spindle

(Rickmyre et al., 2007)

spindle (with Df(2L)H20)

(Rickmyre et al., 2007)

spindle | embryonic stage 4

(Stumpff et al., 2005)

Detailed Description

Statement

Reference

grp⁰⁶⁰³⁴ embryos exhibit shortened division cycles 11-13, as compared to controls.

(Seller et al., 2019)

grp⁰⁶⁰³⁴ third instar larval brains exhibit a similar frequency of cells with chromosome aberrations and no telomeric fusions compared to controls.

(Merigliano et al., 2017)

Cell cycle times are reduced in grp⁰⁶⁰³⁴ embryos, though chromatin remains dispersed until the close of interphase, similar to wild type.

(McCleland et al., 2009)

21.4% of embryos derived from grp⁰⁶⁰³⁴/Df(2L)H20 females and 17.1% of embryos derived from homozygous grp⁰⁶⁰³⁴ females arrest in precortical cycles with acentrosomal, barrel-shaped spindles.

(Rickmyre et al., 2007)

grp⁰⁶⁰³⁴ white pupae are more sensitive to X rays than control white pupae. Treatment of irradiated grp⁰⁶⁰³⁴ larvae with 1.3 μM colchicine significantly decreases their survival (in contrast to irradiated wild-type larvae, where colchicine treatment has little effect on survival). Starvation on a sucrose diet reduces the survival of irradiated mutant larvae.

(Jaklevic et al., 2006)

Mutant eyes (generated using the "EGUF" system) do not display morphological defects when larvae are raised on media containing 2 mmol caffeine/L and 3 mmol hydroxyurea/L.

(Silva et al., 2006)

grp⁰⁶⁰³⁴ flies develop normally and males are fertile.

(Bi et al., 2005)

grp⁰⁶⁰³⁴ embryos display monopolar spindles, the inability to form a central spindle in mitosis of cycle 12 and 13, and the failure to fully separate centrosomes during interphase.

(Stumpff et al., 2005)

Mutants exhibit a robust cell death response to irradiation. Mutants are able to sustain cell proliferation in an organised manner after irradiation. Mutants exposed to 2000R of X-rays delay pupariation with wild-type kinetics.

(Jaklevic and Su, 2004)

Interphase duration after cycle 10 in grp⁰⁶⁰³⁴ embryos extends, but less dramatically than in wild-type embryos.

(Ji et al., 2004)

Mutant has no effect on radiation-induced cell death.

(Xu et al., 2001)

The timing of nuclear envelope formation (NEF) to nuclear envelope breakdown (NEB) is disrupted in cycle 11 and 12 grp⁰⁶⁰³⁴-derived embryos, but the timing of NEB to anaphase initiation (IA) is normal. During cycle 13, the interval between NEF and NEB is 7.3 minutes in grp⁰⁶⁰³⁴-derived embryos (compared to 15.1 minutes in wild-type embryos), the interval between NEB and spindle assembly is not altered, but there is an extensive metaphase delay (the interval between NEB and IA). The chromosomes are not properly condensed at both NEB and metaphase in grp⁰⁶⁰³⁴-derived embryos, although the timing of the initiation of chromosome condensation is normal. The defects in chromosome condensation are most evident during nuclear cycles 12 and 13.

(Yu et al., 2000)

In grp⁰⁶⁰³⁴ embryos, there is an increase in Mitotic index (the proportion of embryos in M Phase) and in the ratio of M phase embryos with unsegregated chromosomes (prophase/metaphase) to those with segregated chromosomes (anaphase/telophase).

(Su et al., 1999)

During somatic cycles 10-13, interphase lengthens progressively. In mutants mitosis 10-13 occurs prematurely. Asynchrony in polar divisions, both among pole nuclei and with respect to somatic divisions still occurs in these embryos.

(Su et al., 1998)

X-irradiation of wild type embryos phenocopies grp⁰⁶⁰³⁴ mutants: sister telophase nuclei snap back and fuse. Embryos also exhibit a dramatic increase in DNA lesions that may be a consequence of grp-derived embryos progressing through mitosis with damaged or incompletely replicated DNA. Alignment of chromosomes on the metaphase plate is disrupted in cycle 11, 12 and 13 embryos.

(Fogarty et al., 1997)

Mutant embryos fail to undergo changes in cell-cycle timing and embryo morphology associated with the midblastula transition (MBT). Interphase length does not increase significantly during divisions 11-13, mitosis 13 is immediately followed by at least two additional syncytial cycles of spindle assembly/disassembly and nuclear envelope breakdown/assembly.

(Sibon et al., 1997)

Embryos from homozygous mothers successfully complete nuclear migration, but undergo abnormal cortical nuclear divisions and do not cellularize.

(Sullivan et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

grp[+]/grp⁰⁶⁰³⁴ is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, p53^UAS.cPa

(Peters et al., 2002)

NOT Suppressor of

Statement

Reference

grp[+]/grp⁰⁶⁰³⁴ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, p53^UAS.cPa

(Peters et al., 2002)

Other

Statement

Reference

grp⁰⁶⁰³⁴, tws⁴³⁰ has lethal - all die before end of larval stage phenotype

(Merigliano et al., 2017)

chico^flp147E, grp⁰⁶⁰³⁴ has lethal | recessive phenotype

(Jaklevic et al., 2006)

Cdk4³, grp⁰⁶⁰³⁴ has lethal | recessive phenotype

(Jaklevic et al., 2006)

CycA^hs.PK, grp⁰⁶⁰³⁴ has lethal phenotype

(Su et al., 1999)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

grp⁰⁶⁰³⁴ has phenotype, non-enhanceable by Wee1^+tPa

(Price et al., 2000)

NOT suppressed by

Statement

Reference

grp⁰⁶⁰³⁴ has phenotype, non-suppressible by Wee1^+tPa

(Price et al., 2000)

NOT Enhancer of

Statement

Reference

grp⁰⁶⁰³⁴ is a non-enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of mre11^unspecified

(Bi et al., 2005)

grp⁰⁶⁰³⁴ is a non-enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefu^unspecified

(Bi et al., 2005)

grp[+]/grp⁰⁶⁰³⁴ is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

grp[+]/grp⁰⁶⁰³⁴ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, p53^UAS.cPa

(Peters et al., 2002)

Suppressor of

Statement

Reference

grp⁰⁶⁰³⁴ is a suppressor | partially of nuclear chromosome | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

grp⁰⁶⁰³⁴ is a suppressor | partially of embryonic/larval brain | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

NOT Suppressor of

Statement

Reference

grp⁰⁶⁰³⁴ is a non-suppressor of egg chorion phenotype of spn-B^BU

(Abdu et al., 2002)

grp⁰⁶⁰³⁴ is a non-suppressor of dorsal appendage phenotype of spn-B^BU

(Abdu et al., 2002)

grp⁰⁶⁰³⁴ is a non-suppressor of egg chorion phenotype of spn-D¹

(Abdu et al., 2002)

grp⁰⁶⁰³⁴ is a non-suppressor of dorsal appendage phenotype of spn-D¹

(Abdu et al., 2002)

grp[+]/grp⁰⁶⁰³⁴ is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

grp[+]/grp⁰⁶⁰³⁴ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, p53^UAS.cPa

(Peters et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Injection of geminin into grp⁰⁶⁰³⁴ embryos results in chromatin condensation behaviour (i.e. very early condensation of chromatin after S phase deletion) and interphase duration indistinguishable from geminin injection into wild type embryos. However, the mitotic delay observed after injection of geminin into grp⁰⁶⁰³⁴ embryos is almost twice that caused by geminin injection into wild type embryos.

(McCleland et al., 2009)

grp⁰⁶⁰³⁴; mei-41^29D flies develop normally and males are fertile. grp⁰⁶⁰³⁴ fails to enhance the chromosome fusion phenotype seen in the nuclei of third instar larval neuroblasts of mre11^unspecified.

(Bi et al., 2005)

A single copy of CycA^hs.PK is sufficient to cause homozygous lethality in grp⁰⁶⁰³⁴ embryos, even without heatshock. A reduction in the dose of CycA fails to suppress the grp⁰⁶⁰³⁴ allele.

(Su et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

grp^bg57

(Fogarty et al., 1997)

Fails to complement

grp^Z5170

(LaRocque et al., 2007)

grp²⁰⁹

(LaRocque et al., 2007)

Comments

Lethality can be rescued by grp cDNA transgene (unspecified).

(Fogarty et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12219

P{PZ}grp⁰⁶⁰³⁴ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

Comments

Revertant analysis confirmed that maternal effect is caused by the P element. Same phenotype observed in embryos derived from homozygous or hemizygous females.

(Sullivan et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

AS6034

Chk1⁰⁶⁰³⁴

(Vasavan et al., 2024)

fs(2)06034

(Spradling et al., 1999)

grapes¹

(Merigliano et al., 2017, Abdu et al., 2002)

grp⁰⁶⁰³⁴

(Seller et al., 2019, McCleland et al., 2009)

grp^I

(Sibon et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (34)

Report Sections

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