FlyBase Allele Report: Dmel\chp[2]

General Information

Symbol

Dmel\chp²

Species

D. melanogaster

Name

FlyBase ID

FBal0030754

Feature type

allele

Associated gene

Dmel\chp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Gurudev et al., 2014)

Mutagen

X ray

(Gurudev et al., 2014)

Progenitor genotype

Cytology

Description

The deletion in chp² starts 3' to exon 6 and removes the remaining coding region. The exact localisation of the second breakpoint could not be determined.

(Gurudev et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

chp protein is abnormallly distributed in chp mutants. In chp¹, levels of protein are reduced along the axons in larvae and pupae. In chp², little or no protein is detected in third instar larval eye disc, in pupa, or in adult retina.

(Pollock et al., 1990)

Normally chp RNA is expressed uniformly throughout photoreceptor cell soma. In chp¹ and chp² mutants, expression is graded and decreases with distance from the nucleus.

(Pollock et al., 1990)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage

(Rosenbaum et al., 2012)

viable

(Gurudev et al., 2014)

Phenotype Manifest In

rhabdomere

(Gurudev et al., 2014, Rosenbaum et al., 2012, Pollock et al., 1990)

rhabdomere microvillus

(Gurudev et al., 2014, Satoh et al., 2013)

rhabdomere of eye photoreceptor cell

(Gurudev et al., 2014)

Detailed Description

Statement

Reference

In mosaic pupal retinas, chp² homozygous photoreceptor cells the stalk and rhabdomeres are properly separated and the microvillar necks are properly developed similar to controls.

(Ogi et al., 2019)

Rudimentary rhabdomeres.

(Gurudev et al., 2014)

Homozygous chp² is viable and show reduction and disorganization of the apical microvilli of rhabdomeres in the adult eye.

chp² mutant photoreceptor cells do not undergo light-dependent degeneration. Despite a nearly complete loss of microvilli, no sign of apoptosis is detected. The phenotype is unaltered under light stress, with or without dietary vitamin A.

(Gurudev et al., 2014)

Rhabdomere microvilli in chp² mutants are relatively short, have variable length and are separated from each other.

(Satoh et al., 2013)

Abnormal rhabdomeric membranes.

(Pollock et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

chp²/chp² is an enhancer of abnormal neuroanatomy | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

chp²/chp² is an enhancer of abnormal neuroanatomy | adult stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

Other

Statement

Reference

Synd^661T, chp² has abnormal neuroanatomy | somatic clone | pupal stage phenotype

(Ogi et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

chp[+]/chp², prom¹ has rhabdomere phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

chp[+]/chp², prom¹ has ommatidium phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

chp², eyc[+]/prom¹ has rhabdomere phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

chp², eyc[+]/prom¹ has ommatidium phenotype, enhanceable by eys[+]/eys¹

(Zelhof et al., 2006)

Enhancer of

Statement

Reference

chp²/chp² is an enhancer of outer photoreceptor cell | adult stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

chp²/chp² is an enhancer of rhabdomere of eye photoreceptor cell | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

chp²/chp² is an enhancer of outer photoreceptor cell | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

chp²/chp² is an enhancer of eye photoreceptor cell stalk | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

chp²/chp² is an enhancer of rhabdomere of eye photoreceptor cell | adult stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

Suppressor of

Statement

Reference

chp[+]/chp² is a suppressor of rhabdomere phenotype of prom¹

(Zelhof et al., 2006)

chp[+]/chp² is a suppressor of ommatidium phenotype of prom¹

(Zelhof et al., 2006)

Other

Statement

Reference

Synd^661T, chp² has rhabdomere of eye photoreceptor cell | somatic clone | pupal stage phenotype

(Ogi et al., 2019)

Synd^661T, chp² has eye photoreceptor cell stalk | somatic clone | pupal stage phenotype

(Ogi et al., 2019)

Synd^661T, chp² has outer photoreceptor cell | somatic clone | pupal stage phenotype

(Ogi et al., 2019)

chp², crb^11A22/crb[+] has rhabdomere of eye photoreceptor cell phenotype

(Gurudev et al., 2014)

chp[+]/chp², crb^8F105 has rhabdomere of eye photoreceptor cell phenotype

(Gurudev et al., 2014)

chp[+]/chp², crb^GX24w- has rhabdomere of eye photoreceptor cell phenotype

(Gurudev et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

In mosaic retinas, chp² and Synd^661T double homozygous photoreceptor cells during pupal stages the stalk-rhabdomere separation is severely and prominently disrupted (even before catacomb-like membrane architecture formation); in adults microvilli show no co-adherence instead spread across the entire apical membrane adult mosaic retina when compared to controls.

(Ogi et al., 2019)

Trans-heterozygous crb^11A22/+ chp² flies exhibit rhabdomeres with significantly smaller width than wild-type. The length of the rhabdomeres is not affected.

(Gurudev et al., 2014)

prom¹/prom¹; chp²/+ double mutant flies show suppression of the prom¹/prom¹ rhabdomeral phenotype. However, in prom¹/prom¹, eys¹/+; chp²/+ flies the rhabdomeres are stuck together, so the mild prom¹/prom¹; chp²/+ phenotype is enhanced in prom¹/prom¹, eys¹/+; chp²/+ flies.

(Zelhof et al., 2006)

In prom¹/prom¹; chp²/+ double mutant flies, the proportion of flies with one aberrant fused cluster of rhabdomeres per ommatidium is smaller than in prom¹/prom¹ single mutants. In the double mutant flies, some ommatidia show the wild-type pattern of 7 individual rhabdomeres, while most ommatidia show between 2 and 5 individual rhabdomeres, and a small proportion show one fused cluster of rhabdomeres. In prom¹/prom¹, eys¹/+; chp²/+ triple mutant flies, the rescue of the prom¹/prom¹ phenotype is reduced compared to the prom¹/prom¹; chp²/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

(Zelhof, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

42251

cu¹ chp²

64127

w^*; sr¹ ninaE¹⁷ e^s chp²

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

chp2

(Pollock et al., 1990)

chp²

(Ogi et al., 2019, Gurudev et al., 2014, Gurudev et al., 2014, Satoh et al., 2013, Rosenbaum et al., 2012, Rosenbaum et al., 2011, Sanxaridis and Tsunoda, 2010, Zelhof et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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