Homozygous somatic clones induced during mid to late third larval instar stage are recovered at a significantly higher frequency than those induced earlier. Although homozygous mutant somatic clones induced after 90h after egg laying can be recovered throughout the anterior wing margin, they are observed at a much higher frequency in the region containing the triple row bristles compared to the double row bristle domain. Clones recovered in the triple row bristle domain tend to be larger than clones in the double row domain. Anterior shifts in cell fates are observed in clones located in the double row bristle domain. Within these clones, margin cells differentiated the thicker triple row bristles instead of the finer bristles typical of the double row. This transformation is cell autonomous and affects all bristles within the clone. shn² clones suffer a growth disadvantage compared to wild type sister clones, and are occasionally absent. When homozygous shn² clones are induced in a Minute background, larger clones are seen. Loss of wing blade tissue and margin structures is also seen. When shn² clones abut or bisect a wing vein, a different class of phenotype is seen. Mutant cells within such clones do not differentiate vein tissue. Clones on the dominant (protruding) surface of the vein are associated with interruptions in the vein tissue. However sometimes, a non-cell autonomous induction of wing vein tissue is observed in the wild-type tissue immediately surrounding the shn² clone. This can lead to branching and disruption of endogenous veins, as well as bifurcations that encapsulate the mutant tissue. In wings in which clones have been induced, small circular arrays of cells which are pigmented like vein cells, but do not posses a compact linear form are seen. These are seen more frequently in the anterior compartment and are encountered both adjacent to endogenous veins and in the intervein region.

At 18^oC head involution is disrupted and larval cuticle associated with an anterior dorsal hole. Embryos are partially ventralised, reduced dorsal cuticle and expansion of ventral denticle belts. At 25^oC dorsal closure fails, dorsal epidermis is significantly reduced. Denticles on the lateral region are abnormally oriented and disrupted.

Homozygous embryos lack dorsal hypoderm.

temperature-sensitive