FlyBase Allele Report: Dmel\Sh[16]

General Information

Symbol

Dmel\Sh¹⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0015556

Feature type

allele

Associated gene

Dmel\Sh

Associated Insertion(s)

?{}Sh¹⁶

Carried in Construct

Also Known As

Sh^M

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Gautam and Tanouye, 1990, Kamb et al., 1987)

Progenitor genotype

Associated Insertion(s)

?{}Sh¹⁶

Cytology

Description

Insertion of 2.2kb DNA.

(Gautam and Tanouye, 1990)

2.2kb insertion at map position +45.5 to +46.1.

(Kamb et al., 1987)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Circelli et al., 2005, Gisselmann et al., 1989)

abnormal behavior | dominant

(Ganetzky and Wu, 1982)

abnormal flight

(Iyengar and Wu, 2014)

abnormal neurophysiology

short lived

(Circelli et al., 2005)

uncoordinated

(Ganetzky and Wu, 1982)

Phenotype Manifest In

axon & motor neuron | conditional ts

(Zhong and Wu, 2004)

Detailed Description

Statement

Reference

Sh¹⁶ mutant flies are capable of sustained flight (for longer than 10 seconds).

Sh¹⁶ mutant flies exhibit a flight pattern that is different to wild-type flies, with increased wing beat frequency but largely normal DLM spiking activity, including an average firing rate and cv of the inter-spike interval.

(Iyengar and Wu, 2014)

Mutant flies exhibit a decrease in the daily sleep amount. This is mainly due to a decrease in the duration of sleep episodes rather than in their number. This phenotype is very sensitive to background modifiers. Those animals that exhibit a short sleeping phenotype also exhibited a shorter lifespan than control siblings.

(Circelli et al., 2005)

At room temperature, the motor axon terminals of Sh¹⁶ larvae show a similar level of arborization to wild-type larvae. However, when larvae are raised at 25^oC, the extent of arborization in Sh¹⁶ motor terminals is greater than that seen in wild type.

(Zhong and Wu, 2004)

Heat-shock induced expression of Sh^29-4.hs in a Sh¹⁶ mutant background results in fast inactivation kinetics and extremely slow recovery from inactivation in mutant neurons.

(Yao and Wu, 2001)

More sensitive than the wild-type to the knock-down effect of acute γ-irradiation.

(Megumi et al., 1995)

"Giant" neurons derived from flies carrying Sh^29-4.hs in a Sh¹⁶ background show a heat shock inducible A-type K⁺ current. This current shows slow recovery from inactivation compared to wild-type.

(Zhao et al., 1995)

I_A is completely eliminated in homozygous third larval instar muscles.

(Haugland and Wu, 1990)

A-type current is completely absent.

(Gisselmann et al., 1989)

Ether-dependent leg shaking and wing scissoring. Chloroform, ethyl acetate and carbon dioxide etherization does not elicit shaking behavior, though nitrogen and triethylkamine etherization does. Unetherized, older flies show uncoordinated walking behavior, and stand quivering on the bottom of the culture bottle.

(Ganetzky and Wu, 1982)

abnormal leg shaking under ether anesthesia; abnormal A-type potassium currents in larval muscle and/or pupal flight muscle; abnormal action potentials in the adult cervical giant fiber; abnormal synaptic transmission at the larval neuromuscular junction and multiple firing of larval motoneurons.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sh¹⁶ has increased rate of movement phenotype, enhanceable by qvr¹

(Wang et al., 2000)

NOT Enhanced by

Statement

Reference

Sh¹⁶ has visible phenotype, non-enhanceable by qvr¹

(Wang et al., 2000)

Enhancer of

Statement

Reference

Sh¹⁶ is an enhancer of increased rate of movement phenotype of qvr¹

(Wang et al., 2000)

NOT Enhancer of

Statement

Reference

Sh¹⁶ is a non-enhancer of visible phenotype of qvr¹

(Wang et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sh¹⁶ has axon & motor neuron | conditional ts phenotype, suppressible by rut¹

(Zhong and Wu, 2004)

Sh¹⁶ has phenotype, suppressible by para^lk5

(Stern et al., 1990)

Additional Comments

Genetic Interactions

Statement

Reference

The increased arborization of motor axon terminals seen when Sh¹⁶ larvae are raised at 25^oC is partially suppressed in rut¹, Sh¹⁶ double mutant larvae.

(Zhong and Wu, 2004)

No wings down phenotype was observed for qvr¹ in combination with Sh²¹, Sh⁵ or Sh¹⁶ though leg shaking is more vigorous in these double mutants than in either of the Sh of qvr single mutants alone. At external Ca²⁺ concentration of 0.4mM, the increase in EJC amplitude caused by qvr¹ is no greater than that caused by Sh¹⁶, and is not enhanced by Sh¹⁶.

(Wang et al., 2000)

The leg shaking phenotype is suppressed by para^lk5.

(Stern et al., 1990)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Ganetzky.

Comments

Phenotype suppressed by mle^nap-ts1, even at permissive temperatures. Shaking and suppressed phenotypes are evident in severed legs as well as in the whole organism.

(Ganetzky and Wu, 1982)

Allelic series of Sh defects: Sh⁷ > Sh¹⁴ > Sh¹⁶ > Sh⁵ > Sh⁹.

(Gisselmann et al., 1989)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sh¹⁶

Sh^M

(Saur et al., 2016, Iyengar and Wu, 2014, Ueda and Wu, 2006, Circelli et al., 2005, Zhong and Wu, 2004, Yao and Wu, 2001, Wang et al., 2000, Renger et al., 1999, Lin and Nash, 1995, Zhao et al., 1995, Zhong et al., 1991, Gautam and Tanouye, 1990, Haugland and Wu, 1990, Stern et al., 1990, Gisselmann et al., 1989, Kamb et al., 1987, Ganetzky and Wu, 1982)

Name Synonyms

Secondary FlyBase IDs

References (20)

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