FlyBase Allele Report: Dmel\otu[7]

General Information

Symbol

Dmel\otu⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0013354

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Oliver et al., 1993)

Mutagen

ethyl methanesulfonate

(Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?424term.

(Glenn and Searles, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,486,864..8,486,864 [-]

Nucleotide change:

A8486864T

Amino acid change:

Reported amino acid change:

?424term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Glenn and Searles, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female

(Tirronen et al., 1993)

decreased cell number | oogenesis

(King et al., 1985)

female sterile

(Sass et al., 1995, Bae et al., 1994)

female sterile | recessive

(Mal'ceva et al., 1997, King et al., 1986)

female sterile germline-dependent | recessive

(Wieschaus et al., 1981)

short lived | female

(Ueyama and Fuyama, 2003)

Phenotype Manifest In

dorsal appendage | ectopic

(Goodrich et al., 2004)

egg

(Goodrich et al., 2004)

egg chamber

(Wieschaus et al., 1981)

nuclear chromosome

(Tirronen et al., 1995)

nurse cell

(Mal'ceva et al., 1997)

nurse cell & nuclear chromosome

(Heino et al., 1995)

nurse cell & nucleus

(Rodesch et al., 1997)

oocyte

(King et al., 1985)

ovariole

(Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Bae et al., 1994)

Detailed Description

Statement

Reference

otu⁷ homozygous females lay only a few eggs, some of which have a 'crown' of dorsal appendage material, indicating dorsalisation.

(Goodrich et al., 2004)

Homozygous females have a shorter mean life span compared to heterozygous controls. The lifespan of mated homozygous females is significantly shorter than that of unmated homozygous females.

(Ueyama and Fuyama, 2003)

Homozygous and otu⁷/otu¹¹ females do produce differentiated egg chambers.

(Van Buskirk and Schupbach, 2002)

The nurse cells of homozygous females contain giant polytene chromosomes of various morphologies. otu⁷/otu¹¹ females are fertile and their nurse cells often contain polytene chromosomes, which are comparable to and often larger than normal salivary gland chromosomes.

(Mal'ceva et al., 1997)

Nurse cell nuclei often appear misplaced compared to wild-type and some appear to block ring canal openings.

(Rodesch et al., 1997)

Chromatin organization in the pseudonurse cells differs from wild type. Instead of showing endopolyploidy, the chromosomes are polytenised.

(Heino et al., 1995)

Differentiated egg cysts are arrested at late oogenic stages.

(Rodesch et al., 1995)

DIF (differentiated) class allele. The homologous chromosomes of the nurse cells do not dissociate after stage 4 as in wild-type animals, but remain conjoined and become polytenised as the egg chambers mature.

(Tirronen et al., 1995)

Late stage arrest of oogenesis.

(Bae et al., 1994)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

93% of homozygous ovarioles produce nurse cell/oocyte syncytia, and 5% have quiescent oogonia. Most otu⁷/otu¹¹ female flies are fertile.

(Storto and King, 1987)

Differentiated allele; 93% of homozygous ovarioles contain differentiated cystocytes forming oocytes. 99% of hemizygous ovarioles are quiescent.

(King et al., 1986)

Oocyte differentiation is destabilized in certain otu alleles; for example, the presumptive oocytes in about 20% of otu⁷ homozygotes resemble nurse cells in their polytenization, although they lag behind the remaining nurse cells by at least one replication cycle.

(King et al., 1985)

The egg chambers become abnormal on entering vitellogenesis in homozygous ovaries.

(Wieschaus et al., 1981)

differentiated; ovarian chambers with pseudonurse cells or nurse cell/oocyte synctia

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

otu[+]/otu⁷ is a suppressor of abnormal mitotic cell cycle phenotype of enc^UU3

(Van Buskirk and Schupbach, 2002)

Phenotype Manifest In

Suppressor of

Statement

Reference

otu[+]/otu⁷ is a suppressor of egg chamber phenotype of enc^UU3

(Van Buskirk and Schupbach, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The extra germline cell phenotype seen in enc^UU3 egg chambers is suppressed by otu⁷/+.

(Van Buskirk and Schupbach, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Nrg^ibx

(Carhan et al., 2005)

Rescued by

otu⁷ is rescued by otu^+t5.4

(Comer et al., 1992)

Partially rescued by

otu⁷ is partially rescued by otu⁴²³

(Glenn and Searles, 2001)

otu⁷ is partially rescued by otu⁶²⁷

(Glenn and Searles, 2001)

Not rescued by

otu⁷ is not rescued by otu³³⁸

(Glenn and Searles, 2001)

otu⁷ is not rescued by otu²⁵⁰

(Glenn and Searles, 2001)

otu⁷ is not rescued by otu³⁸⁰

(Glenn and Searles, 2001)

Comments

The reduced female receptivity phenotype of Nrg^ibx is complemented by otu⁷.

(Carhan et al., 2005)

Rescued by otu^+t5.4 : fertility and normal ovarian development is restored.

(Comer et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (2)

3146

106878

Notes on Origin

Discoverer

Engstrom.

Comments

Complements: ibx^M72.

(Kerr et al., 1997)

Germ line clonal analysis shows that otu function is required in the germ line.

(Wieschaus et al., 1981)

Differentiated (DIF) class allele. The ovarian phenotype of flies transheterozygous for otu⁷ and a number of other otu mutations has been analysed.

(Storto and King, 1987)

One of DIF class of alleles.

(Comer et al., 1992)

Shows a dominant interaction with ovo^D2 and ovo^D3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles. Reverse transcription and PCR, and in situ hybridization with the male specific exon, showed that the Sxl transcripts are spliced in the male-specific mode in transformed germ cells. This effect is alleviated by the Sxl^M1 mutation.

(Pauli et al., 1993)

Strong DIF class of otu allele. P{otu-98} partially restores fertility, P{otu-104} completely restores fertility.

(Sass et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

1304

(Mohler and Carroll, 1984)

fs(1)1304

(Tran et al., 1998, Wieschaus et al., 1981)

fs(1)1304b

fs1304

(King et al., 1986)

otu7

(Le Thomas et al., 2013)

otu⁷

(Casier et al., 2023, Ji et al., 2019, Popodi et al., 2010-, Christensen et al., 2008.12.28, Cook et al., 2008.12.28, Carhan et al., 2005, Ueyama and Fuyama, 2003)

Name Synonyms

Secondary FlyBase IDs

References (35)

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