FB2024_03 , released June 25, 2024
Allele: Dmel\Nl1N-3
Open Close
General Information
Symbol
Dmel\Nl1N-3
Species
D. melanogaster
Name
FlyBase ID
FBal0012878
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
Also Known As
l(1)N3
Key Links
Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(de Celis et al., 1996)
Mutagen
gamma ray
Progenitor genotype
Cytology
Description

Nl1N-3 contains two lesions in N; a proximal frameshift (the same lesion as in NMcd3) and a distal regulatory lesion.

(Ramain et al., 2001)

2bp deletion at nucleotide 16682, resulting in a frameshift adding 46 unique amino acids and terminating at codon 2528.

(Brennan et al., 1997)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In homozygous clones in the leg that span a joint, the homozygous cells do not form any joint tissue, although wild-type cells that are in contact with the clone can form joint structures, resulting in incomplete joints. The effect of the Nl1N-3 mutation on joint formation appears to be autonomous. Clones in the anterior-ventral compartment of the femur and tibia interfere with the separation between the distal femur and the proximal tibia. Legs carrying large anterior or posterior clones are shorter than their wild-type counterparts, and mosaic tarsal segments have a 25% reduction in area and a 30% reduction in length compared to wild-type controls. Clones restricted to one leg segment do not affect the size of this segment or the overall morphology and size of the leg.

      (de Celis et al., 1998)

      Heterozygotes appear wild-type.

      (Brennan et al., 1997)

      Homozygous clones on the ventral surface of the wing abutting the wing margin cause extensive scalloping, dorsal clones differentiate a normal margin. Mosaic wings also exhibit altered spacing between the veins.

      (de Celis et al., 1996)

      Homozygous lethal at 29oC. Homozygotes do not reach the late pupal stage. 14% of Nl1N-3/Nl1N-ts1 flies survive at 29oC. Phenotypes seen include fused stubby legs, a small head and small, rough eyes. 29% of Nl1N-3/NAx-tsl flies survive at 29oC. Phenotypes seen include small eyes and head, an abruptex phenotype and stubby legs.

      (Shellenbarger and Mohler, 1975)

      Nl1N-3/+ females are wild type; Nl1N-3/N females and Nl1N-3/Y males are lethal. Heterozygotes with fa and Nfa-g are fa-like; with nd, they have nd-like wings and small eyes; with Nnd-3, they are viable, fertile and Nnd-3-like. Developmental defects in Nl1N-3/Y males and time of lethal effect same as in Nl1N-1/Y. Some Nl1N-3/Nl1N-ts1 females survive to late pupal stage (Shellenbarger and Mohler, 1975).

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Nl1N-3 ; wgS107 double heterozygotes have a reduced number of thoracic bristles compared to wild-type. Nl1N-3 ; Df(3R)Dl-FX3 double heterozygotes have an increased number of thoracic bristles compared to wild-type.

      (Brennan et al., 1997)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Abrahamson.

      Comments
      Comments

      Intragenic recombination experiments indicate that the proximal frameshift lesion in Nl1N-3 is associated with pupal lethality and a dominant loss of microchaetae phenotype, while the distal regulatory lesion is associated with embryonic neurogenic lethality and acts as an intragenic suppressor of the dominant loss of microchaetae phenotype caused by the proximal lesion.

      (Ramain et al., 2001)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Nl1N-3
      l(1)N3
      (de Celis et al., 1998, Brennan et al., 1997, de Celis et al., 1996, Shellenbarger and Mohler, 1975)
      l(3)N3
      (Ramain et al., 2001)
      lN3
      Name Synonyms
      Secondary FlyBase IDs
        References (9)