FlyBase Allele Report: Dmel\BRWD3[s5349]

General Information

Symbol

Dmel\BRWD3^s5349

Species

D. melanogaster

Name

FlyBase ID

FBal0011165

Feature type

allele

Associated gene

Dmel\BRWD3

Associated Insertion(s)

P{lacW}BRWD3^s5349

Carried in Construct

Also Known As

ram^p2, dBRWD3^s5349

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(D'Costa et al., 2006)

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}BRWD3^s5349

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin filament

(Chen et al., 2015)

cone cell | somatic clone

(Chen et al., 2015)

nucleus

(Chen et al., 2015)

photoreceptor | somatic clone

(Chen et al., 2015)

Detailed Description

Statement

Reference

In BRWD3^s5349 mutant clones, the photoreceptors project axons to the brain normally, but do not differentiate into mature neurons. The differentiation of R7 and cone cells is arrested. At the adult stage the number of surviving BRWD3^s5349 mutant ommatidia is much smaller than in wild type controls.

BRWD3^s5349 mutant eye disc clones exhibit defects in nuclear migration and actin cytoskeleton organisation.

(Chen et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BRWD3^s5349 has abnormal neuroanatomy | somatic clone phenotype, suppressible by yem^GS21861

(Chen et al., 2015)

BRWD3^s5349 has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, suppressible by yem^GS21861

(Chen et al., 2015)

NOT suppressed by

Statement

Reference

BRWD3^s5349 has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, non-suppressible by XNP³

(Chen et al., 2015)

NOT Suppressor of

Statement

Reference

BRWD3^s5349/BRWD3[+] is a non-suppressor of visible phenotype of hh^bar3/hh⁸

(D'Costa et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

BRWD3^s5349 has photoreceptor | somatic clone phenotype, suppressible by yem^GS21861

(Chen et al., 2015)

BRWD3^s5349 has eye disc | somatic clone phenotype, suppressible by yem^GS21861

(Chen et al., 2015)

NOT suppressed by

Statement

Reference

BRWD3^s5349 has eye | somatic clone phenotype, non-suppressible by XNP³

(Chen et al., 2015)

Other

Statement

Reference

BRWD3^s5349/BRWD3[+], hh^bar3/hh⁸ has rhabdomere phenotype

(D'Costa et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous yem^GS21861 suppresses the photoreceptor differentiation defects and cell lethality seen in BRWD3^s5349 mutant eyes clones. yem^GS21861 also suppresses the nuclear migration and actin cytoskeleton defects seen in BRWD3^s5349 mutant eye disc clones.

Homozygous XNP³ fails to suppress the cell lethality seen in BRWD3^s5349 mutant eye clones.

(Chen et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

BRWD3^ram1

(D'Costa et al., 2006)

BRWD3⁰⁵⁸⁴²

(BDGP Project Members, 1994-1999)

Rescued by

BRWD3^s5349 is rescued by BRWD3^{Ubi-p63E.mRFP1.Tag:FLAG}

(Chen et al., 2015)

Not rescued by

BRWD3^s5349 is not rescued by BRWD3^{delta-N.Ubi.Tag:FLAG.mRFP1}

(Chen et al., 2015)

Comments

Expression of BRWD3^{Ubi-p63E.T:Disc\RFP.T:Zzzz\FLAG} rescues the R7 photoreceptors and cone cell differentiation in BRWD3^s5349 mutant clones and rescues the lethality of BRWD3^s5349 mutants.

Expression of BRWD3^{delta-N.Ubi.T:Zzzz\FLAG.T:Disc\RFP} fails to rescue the R7 photoreceptors and cone cell differentiation defects in BRWD3^s5349 mutant clones. It also fails to rescue the lethality of BRWD3^s5349 mutants.

(Chen et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35824

w^*; P{neoFRT}82B P{lacW}BRWD3^s5349/TM2

Notes on Origin

Discoverer

M. Scott.

Comments

Complements: crb⁰⁷²⁰⁷.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

BRWD3^s5349

dBRWD3^s5349

(Shih et al., 2016, Chen et al., 2015)

l(3)05842^s5349

(D'Costa et al., 2006, BDGP Project Members, 1994-1999)

l(3)s5349

(Spradling et al., 1999)

l(3)s5349^s5349

ram^P2

(D'Costa et al., 2006)

ram^p2

(Ihry and Bashirullah, 2014)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close