FlyBase Allele Report: Dmel\Pfdn2[01239]

General Information

Symbol

Dmel\Pfdn2⁰¹²³⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0009405

Feature type

allele

Associated gene

Dmel\Pfdn2

Associated Insertion(s)

P{PZ}Pfdn2⁰¹²³⁹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Pfdn2⁰¹²³⁹

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:11,074,285..11,074,285 [+]

(Makunin et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitotic spindle | third instar larval stage (with Df(3L)BSC457)

(Zhang et al., 2016)

neuroblast | increased number | third instar larval stage (with Df(3L)BSC457)

(Zhang et al., 2016)

neuroblast | increased number | third instar larval stage (with Pfdn2^Δ10)

(Zhang et al., 2016)

Detailed Description

Statement

Reference

Pfdn2⁰¹²³⁹/Df(3L)BSC457 mutants show ectopic neuroblasts in third instar larval brains and an increase in the number of cells in S-phase (assessed by EdU labelling).They also display defects in asymmetric protein segregation (both in metaphase and telophase) and mitotic spindle orientation in neuroblasts.

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ transheterozygotes display increased average number of brain neuroblasts compared to wild-type.

(Zhang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by aPKC^GD1366/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has increased cell number | third instar larval stage phenotype, suppressible | partially by aPKC^GD1366/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by par-6^GD4048/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has increased cell number | third instar larval stage phenotype, suppressible | partially by par-6^GD4048/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has neuroblast | increased number | third instar larval stage phenotype, suppressible | partially by aPKC^GD1366/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Pfdn2^Δ10/Pfdn2⁰¹²³⁹ has neuroblast | increased number | third instar larval stage phenotype, suppressible | partially by par-6^GD4048/Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dramatically increased average number of brain neuroblasts characteristic for Pfdn2^Δ10/Pfdn2⁰¹²³⁹ transheterozygote third instar larvae is partially restored by expression of either aPKC^GD1366 or par-6^GD4048 under the control of Scer\GAL4^insc-Mz1407 in the mutant background.

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Pfdn2^j9B4

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11526

P{PZ}Pfdn2⁰¹²³⁹ ry⁵⁰⁶/TM6B, ry^CB Tb¹ ca¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: SuUR^ES.

(Makunin et al., 2002)

Complements: l(3)03691⁰³⁶⁹¹. Complements: klu⁰⁹⁰³⁶. Complements: klu¹⁰⁰⁵².

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P01239

(Makunin et al., 2002)

Pfdn2⁰¹²³⁹

l(3)01239

(Spradling et al., 1999)

l(3)01239⁰¹²³⁹

(Christensen et al., 2008.4.15)

pfdn2⁰¹²³⁹

(Zhang et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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