FlyBase Allele Report: Dmel\osa[00090]

General Information

Symbol

Dmel\osa⁰⁰⁰⁹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0009367

Feature type

allele

Associated gene

Dmel\osa

Associated Insertion(s)

P{PZ}osa⁰⁰⁰⁹⁰

Carried in Construct

Also Known As

l(3)00090, osa¹³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Treisman et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}osa⁰⁰⁰⁹⁰

Cytology

Description

P{PZ} insertion 780bp upstream of the first Met residue, in the first intron. The P{PZ} element is inserted in the opposite orientation to the direction of osa transcription.

(Vazquez et al., 1999)

P{PZ} insertion in the first intron.

(Carrera et al., 1998)

P{PZ} insertion in the first intron, downstream of a non-coding exon.

(Treisman et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:17,717,227..17,717,227 [+]

(Tian and Smith-Bolton, 2021, Nakamura et al., 2008, Ragab et al., 2006, Brumby et al., 2002, Abrell et al., 2000, Spradling et al., 1999, Vazquez et al., 1999, Carrera et al., 1998, Treisman et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intellectual disability

modeled by Hsap\MECP2^UAS.FL

(Vonhoff et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999)

Phenotype Manifest In

Malpighian tubule tip cell

(Carrera et al., 1998)

Detailed Description

Statement

Reference

Each Malpighian tubule develops two tip cells in osa⁰⁰⁰⁹⁰/osa^dust55 and osa⁰⁰⁰⁹⁰/osa^dust31 embryos.

(Carrera et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

osa⁰⁰⁰⁹⁰/osa[+] is an enhancer of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

osa⁰⁰⁰⁹⁰ is an enhancer of visible | dominant phenotype of Kr^If-1

(Abrell et al., 2000)

osa⁰⁰⁰⁹⁰/osa[+] is an enhancer of visible | dominant phenotype of Kr^If-1

(Carrera et al., 1998)

Suppressor of

Statement

Reference

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of abnormal neuroanatomy phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Vonhoff et al., 2012)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^ChAT.7.4

(Cukier et al., 2008)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of abnormal eye color phenotype of Hsap\MECP2^R294X.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

osa⁰⁰⁰⁹⁰/osa[+] is an enhancer of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

osa⁰⁰⁰⁹⁰ is an enhancer of eye phenotype of Kr^If-1

(Abrell et al., 2000)

osa⁰⁰⁰⁹⁰ is an enhancer of ommatidium phenotype of Kr^If-1

(Abrell et al., 2000)

osa⁰⁰⁰⁹⁰/osa[+] is an enhancer of eye phenotype of Kr^If-1

(Carrera et al., 1998)

NOT Enhancer of

Statement

Reference

osa⁰⁰⁰⁹⁰/osa[+] is a non-enhancer of leg phenotype of e(y)3⁴

(Chalkley et al., 2008)

osa⁰⁰⁰⁹⁰/osa[+] is a non-enhancer of wing vein | ectopic phenotype of net¹

(Marenda et al., 2004)

Suppressor of

Statement

Reference

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of indirect flight muscle motor neuron DLMN5 phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Vonhoff et al., 2012)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of dendrite phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Vonhoff et al., 2012)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of eye phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of wing vein | increased number phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^cv-c-C5

(Cukier et al., 2008)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor of eye phenotype of Hsap\MECP2^Δ166.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

osa⁰⁰⁰⁹⁰/osa[+] is a suppressor | partially of eye phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Nakamura et al., 2008)

osa⁰⁰⁰⁹⁰ is a suppressor of eye phenotype of CycE^JP

(Brumby et al., 2002)

NOT Suppressor of

Statement

Reference

osa⁰⁰⁰⁹⁰/osa[+] is a non-suppressor of wing vein | ectopic phenotype of net¹

(Marenda et al., 2004)

Other

Statement

Reference

Df(2R)ED3921/+, osa⁰⁰⁰⁹⁰ has wing phenotype

(Ragab et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is ameliorated by combination with a single copy of osa⁰⁰⁰⁹⁰.

(Morimoto et al., 2016)

Flies heterozygous for osa⁰⁰⁰⁹⁰ show suppression of the Scer\GAL4^GMR.PS, Dref^Scer\UAS.cSa severe rough eye phenotype; eyes are modestly rough and fused ommatidia are not apparent.

(Nakamura et al., 2008)

35% of Df(2R)ED3921/+; osa⁰⁰⁰⁹⁰/+ flies show a held out wing phenotype, while neither single mutant shows this phenotype. The penetrance is increased to 100% in Df(2R)ED3921; osa⁰⁰⁰⁹⁰/+ flies. The wing vein phenotype of Df(2R)ED3921 single mutants is enhanced by osa⁰⁰⁰⁹⁰, resulting in loss of L5 up to the posterior crossvein.

(Ragab et al., 2006)

Suppresses the CycE^JP rough eye phenotype.

(Brumby et al., 2002)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is ameliorated by combination with a single copy of osa⁰⁰⁰⁹⁰.

(Morimoto et al., 2016)

Introduction of osa⁰⁰⁰⁹⁰/+ significantly increases total MN5 dendritic length and dendritic number above that in the Hsap\MECP2^Scer\UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^Cha.3.3P background.

(Vonhoff et al., 2012)

Complementation and Rescue Data

Fails to complement

osa^L6530b

(BDGP Project Members, 1994-1999)

osa^s3263b

(BDGP Project Members, 1994-1999)

osa⁰⁴⁵³⁹

(BDGP Project Members, 1994-1999)

osa^j9C3

(BDGP Project Members, 1994-1999)

osa⁰⁶⁷²⁶