FlyBase Allele Report: Dmel\PolG1[3]

General Information

Symbol

Dmel\PolG1³

Species

D. melanogaster

Name

FlyBase ID

FBal0008416

Feature type

allele

Associated gene

Dmel\PolG1

Associated Insertion(s)

Carried in Construct

Also Known As

tam³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Woodruff and Ashburner, 1979)

Progenitor genotype

Cytology

Description

5bp deletion (of nucleotides 3371 to 3375). Deletion is in exon 3 just after conserved DNA polymerase domain Z and results in a frameshift of the rest of the protein.

(Iyengar et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:13,824,320..13,824,323 [-]

Comment:

Mutation is a 5bp deletion just after conserved DNA polymerase domain Z and results in a frameshift of the rest of the protein.

(Iyengar et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage | recessive

(Iyengar et al., 1999)

abnormal neuroanatomy | third instar larval stage (with PolG1⁹)

(Baqri et al., 2009)

lethal (with Df(2L)Exel7059)

(Kahsai and Cook, 2018)

lethal | recessive

(Woodruff and Ashburner, 1979)

lethal - all die before end of larval stage | recessive

(Iyengar et al., 1999)

lethal - all die before end of P-stage (with Df(2L)BSC252)

(Bratic et al., 2015)

lethal - all die before end of P-stage (with Df(2L)Exel7059)

(Bratic et al., 2015)

lethal - all die before end of P-stage (with PolG1⁴)

(Bratic et al., 2015)

lethal - all die before end of P-stage (with PolG1^D263A)

(Bratic et al., 2015)

lethal - all die before end of P-stage (with PolG1^H1038A)

(Bratic et al., 2015)

lethal - all die before end of P-stage (with PolG1^Q1009A)

(Bratic et al., 2015)

some die during larval stage | recessive

(Iyengar et al., 1999)

Phenotype Manifest In

embryonic/larval motor neuron | third instar larval stage

(Baqri et al., 2009)

embryonic/larval motor neuron | third instar larval stage (with PolG1⁹)

(Baqri et al., 2009)

eye disc

(Iyengar et al., 1999)

larval segmental nerve | third instar larval stage (with PolG1⁹)

(Baqri et al., 2009)

mitochondrion | third instar larval stage

(Baqri et al., 2009)

mitochondrion | third instar larval stage (with PolG1⁹)

(Baqri et al., 2009)

photoreceptor cell & axon

(Iyengar et al., 1999)

photoreceptor neuron

(Iyengar et al., 1999)

ventral oblique muscle cell | third instar larval stage (with PolG1⁹)

(Baqri et al., 2009)

Detailed Description

Statement

Reference

tam³/tam^D263A, tam³/tam^Q1009A, tam³/tam^H1038A, tam³/Df(2L)Exel7059, tam³/Df(2L)BSC252 and tam³/tam⁴ mutants exhibit complete lethality.

(Bratic et al., 2015)

Mitochondria in muscle fibres 6/7 in abdominal segments of tam³/tam⁹ mutant third instar larvae exhibit decreased mtDNA content, increased mitochondrial density and mitochondria appear abnormally tightly packed instead of arranged in normal banded patterns, but there is no qualitative difference in mitochondrial morphology when examined via electron microscopy, as compared to controls. Third instar larvae display significantly higher mitochondrial density in the proximal region of the segmental nerve, but not in the medial or distal regions. Mitochondrial flux in tam³/tam⁹ third instar motor neurons is significantly increased in both directions, and there is no difference in retrograde velocity, but a significant decrease in anterograde velocity, as compared to controls.

There is no significant difference in synaptic vesicle precursor flux or velocity in tam³/tam⁹ or tam³/+ third instar motor neurons, as compared to controls.

Mitochondrial flux in third instar tam³/+ motor neurons is significantly increased in both directions, and there is no difference in retrograde velocity of mitochondria, but there is a significant decrease in anterograde velocity, as compared to controls.

(Baqri et al., 2009)

Hemizygous lethality occurs during the late third larval instar stage. tam³/tam⁹ larvae fail to wander at the appropriate time. tam⁹/tam³ larvae have smaller eye discs than normal and show delayed photoreceptor differentiation. The area of differentiated photoreceptors is abnormal and their projection pattern is abnormal. tam³/tam⁹ larvae move significantly less than wild-type larvae.

(Iyengar et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

PolG1¹⁰

(Iyengar et al., 1999)

Fails to complement

PolG1⁹

(Iyengar et al., 1999)

PolG1⁴

(Iyengar et al., 1999)

PolG1²

(Iyengar et al., 1999)

Comments

tam^Rescue complements tam³ to produce viable flies.

(Bratic et al., 2015)

Homozygous tam⁹ larvae show no response to light in contrast to wild-type larvae. Larvae transheterozygous for tam⁹ and tam³ respond to light, suggesting partial complementation of this phenotype.

(Iyengar et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

3410

PolG1³ b¹ Adhⁿ⁴/CyO

Kyoto

107034

PolG1³ b¹ Adhⁿ⁴/CyO

101317

b¹ PolG1³ Adhⁿ⁴ / CyO, Duox^Cy dpy^lv1 pr¹ cn²

Notes on Origin

Discoverer

Ashburner.

Separable from: a second-site mutation. Probably associated with a second-site mutation, since homozygotes die during late embryonic stages whereas hemizygotes die during the late third larval instar stage.

(Iyengar et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

PolG1³

Polγ-α³

(Meng et al., 2017)

SF23

l(2)34Dc³

(Ashburner et al., 1999.10.12)

l(2)br14^SF23

(Woodruff and Ashburner, 1979)

tam3

(Bratic et al., 2015)

tam³

(Chiang et al., 2019, Kahsai and Cook, 2018, Baqri et al., 2009, Chen et al., 2008, Christensen and Cook, 2007.3.22)

tamas³

Name Synonyms

Secondary FlyBase IDs

References (11)

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