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Christensen, S., Cook, K. (2007.3.22). Isolation and characterization of Df(2L)BSC252. 
FlyBase ID
FBrf0199241
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Personal communication to FlyBase
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Text of Personal Communication
Date: Thu, 22 Mar 2007  14:52:06  -0400
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC252
Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufman@XXXX
Isolation and characterization of Df(2L)BSC252
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC252 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}Sos[f05577] and P{XP}d02133. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}Sos[f05577]/P{XP}d02133 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC252 from the segment 
of PBac{WH}Sos[f05577] to the left of its FRT site and the segment of 
P{XP}d02133 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(2L)BSC252 predicted from the Release 4 
genomic coordinates of the transposable element insertions sites are 
34D1;34F1. It failed to complement tam[3], Ance[34Eb-2] and rk[4].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    English
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    Aberrations (1)
    Alleles (3)
    Genes (3)
    Insertions (3)
    Transgenic Constructs (1)