FB2024_03 , released June 25, 2024
Allele: Dmel\eve1
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General Information
Symbol
Dmel\eve1
Species
D. melanogaster
Name
FlyBase ID
FBal0003883
Feature type
allele
Associated gene
Dmel\eve
Associated Insertion(s)
Carried in Construct
Also Known As
eveID19, eveID
Key Links
Genomic Maps

Allele class

heat sensitive loss of function allele

Mutagen
Nature of the Allele
Allele class

heat sensitive loss of function allele

(Frasch et al., 1988)
Mutagen
ethyl methanesulfonate
(Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Nucleotide substitution: G to A. Amino acid replacement: R121H. R121H replacement is near the putative DNA-binding helix of the homeobox.

(Frasch et al., 1988)

arg121 --> his

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:9,979,847..9,979,847 [+]
Nucleotide change:

G9979847A

Amino acid change:

R121H | eve-PA

(Frasch et al., 1988)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal anterior fascicle & abdominal segment 3

      (Landgraf et al., 1999)

      abdominal anterior fascicle & abdominal segment 4

      (Landgraf et al., 1999)

      abdominal anterior fascicle & abdominal segment 5

      (Landgraf et al., 1999)

      abdominal segment 3 & somatic muscle

      (Landgraf et al., 1999)

      abdominal segment 4 & somatic muscle

      (Landgraf et al., 1999)

      abdominal segment 5 & somatic muscle

      (Landgraf et al., 1999)

      embryo

      (Hughes and Krause, 2001)

      embryonic/first instar larval cuticle

      (Hughes and Krause, 2001, Kobayashi et al., 2001)

      embryonic/larval somatic muscle cell

      (Su et al., 1999)

      embryonic/larval visceral muscle cell

      (Su et al., 1999)

      embryonic segment

      (Hughes and Krause, 2001, Frasch et al., 1988)

      larval brain & neuroblast (with eve5)

      (Park et al., 2001)

      larval RP2 motor neuron

      (Su et al., 1999)

      muscle cell of abdominal 2 dorsal acute muscle 1

      (Su et al., 1999)

      muscle cell of abdominal 3 dorsal acute muscle 1

      (Su et al., 1999)

      muscle cell of abdominal 4 dorsal acute muscle 1

      (Su et al., 1999)

      muscle cell of abdominal 5 dorsal acute muscle 1

      (Su et al., 1999)

      muscle cell of abdominal 6 dorsal acute muscle 1

      (Su et al., 1999)

      muscle cell of abdominal 7 dorsal acute muscle 1

      (Su et al., 1999)

      parasegment

      (Hughes and Krause, 2001, Kobayashi et al., 2001)

      parasegment 3

      (Hughes and Krause, 2001)

      parasegment 4

      (Hughes and Krause, 2001)

      parasegment 5

      (Hughes and Krause, 2001)

      parasegment 6

      (Hughes and Krause, 2001)

      pericardial cell

      (Su et al., 1999)

      presumptive embryonic/larval nervous system

      (Su et al., 1999)

      ventral denticle belt

      (Carroll and Scott, 1986)
      Detailed Description
      Statement
      Reference

      Embryos have a pair rule phenotype. The segments that remain are about 1.4 times wider than wild-type segments and the cuticle is about 70% the length of a wild-type cuticle. The number of embryos that hatch and the number of adults eclosing is dramatically reduced compared to controls. ftz-dependent parasegments are enlarged and eve-dependent parasegments are reduced in stage 8 mutant embryos. The relative widths of enlarged and reduced parasegments remain relatively constant until germ-band retraction. Between stages 12-15 the relative widths of the smaller parasegments begin to decrease and by stage 16 reduced parasegments are no longer seen and the embryo is composed of seven enlarged parasegments. Parasegments 3 to 6 are unevenly spaced at stage 6-7 with parasegments 4 and 6 being about 1.4 times wider than wild-type and parasegments 3 and 5 being about 0.6 times the wild-type width. The wider parasegments contain about twice the number of cells of the narrower parasegments. The total width and number of cells in all four parasegments is equivalent to wild type. Each of the narrow parasegments maintains a constant relative width until around stage 12. After this time they decrease further in both width and cell number. The wide parasegments remain about 1.3-1.5 times wider and contain about 1.4 times as many cells as a normal parasegment. The pattern of cell death is abnormal in mutant embryos. Increased levels of apoptosis do not appear to be induced at or prior to stage 11 despite the earlier changes in parasegment size. By the end of stage 12 however, higher than normal levels of cell death are seen. The dying cells at this stage are almost exclusively in the wider parasegments, with few, if any, dying cells in the narrow parasegments. By stage 14, overall levels of apoptosis within the ectodermal layer have decreased. However, clusters of dying cells begin to appear below the surface of the narrow parasegments. These clusters increase in size and number at stage 15. At stage 12, cells of the narrow parasegments are seen to delaminate and move interiorly. The majority of these cells do not die until they have delaminated from the ectodermal layer, at stage 14, when the large clusters of dying cells begin to appear below the ectodermal surface.

      (Hughes and Krause, 2001)

      Homozygous embryos have abnormally narrow odd-numbered parasegments at early stages of development which are eliminated at later stages.

      (Kobayashi et al., 2001)

      eve1/eve5 larval brains show a significant reduction in normalised proliferation compared to controls.

      (Park et al., 2001)

      Homozygous embryos show muscle abnormalities in abdominal segments 2 and 6. When mutant embryos are shifted to the restrictive temperature at 5 hours after egg laying most eve-expressing neurons form but the dorsal projections of motor axons are abnormal. The ISN is arrested prematurely in the ventral or dorsolateral region of the muscle field. By 9-10 hours AEL removal of eve function rarely affects ISN formation. Dorsal muscles form normally.

      (Landgraf et al., 1999)

      When eve1 embryos are shifted to the non-permissive temperature for 2 hours at early stage 11, the number of eve-expressing pericardial cells (EPCs) is reduced to on average only 24% of the wild-type number. Some of the EPCs that are present are located at some distance from the heart tube. DA1 muscle formation is also affected, but to a lesser degree. The reduction in the number of EPCs is less severe if the temperature shifts are earlier or later in development. The number of cardial cells, heart tube formation and overall body muscle formation are not significantly affected in eve1 embryos shifted to the restrictive temperature at early stage 11. Embryos shifted to the restrictive temperature at stage 11 show no morphologically detectable central nervous system defects. Temperature shifts at earlier stages perturb neural development (for example the RP2 neurons) and perturb visceral and somatic muscle formation.

      (Su et al., 1999)

      Weak allele at 18oC, moderate allele at 29oC. At 18oC even-numbered denticle belts are variably deleted in homozygous embryos. At 29oC the frequency of deletions increases. At 31oC the ventral epidermis secretes a continuous lawn of denticles, which are derived from the 'fusion' of odd-numbered denticle belts. The phenotype of eve1 odd5 double homozygotes at 29oC is not an additive effect of the two mutations; the double mutant has 7 or 8 partial denticle belts, each with an apparent polarity reversal.

      (Coulter and Wieschaus, 1988)

      At the restrictive temperature of 31oC, homozygous embryos have a 'lawn of denticles' phenotype. At 25oC, homozygous embryos show a pair-rule phenotype. At 14 or 18oC, homozygous embryos show essentially normal segmentation.

      (Frasch et al., 1988)

      Even numbered denticle bands missing.

      (Jack et al., 1988)

      Unsegmented lawn of denticle bands.

      (Carroll and Scott, 1986)

      temperature-sensitive allele

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      eve1 is an enhancer of lethal | embryonic stage | maternal effect phenotype of Gug03928

      (Zhang et al., 2002)

      eve1 is an enhancer of lethal | embryonic stage | maternal effect phenotype of Guge46-2

      (Zhang et al., 2002)

      eve1 is an enhancer of lethal | embryonic stage | maternal effect phenotype of Gug01323a

      (Zhang et al., 2002)

      eve1 is an enhancer of lethal | embryonic stage | maternal effect phenotype of GugrO154a

      (Zhang et al., 2002)

      eve[+]/eve1 is an enhancer of partially lethal - majority die phenotype of trol13

      (Park et al., 1998)
      Other
      Statement
      Reference

      eve1, ftz3-413.hs has lethal phenotype

      (Hughes and Krause, 2001)
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Statement
      Reference

      eve[+]/eve1 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, fruNP0021

      (Goto et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      An eve1 background enhances the rough eye phenotype related to fru isoform B, found upon expression of fruNP0021 under the control of Scer\GAL4GMR.PF.

      (Goto et al., 2011)

      The number of eve1 ftz3-413.hs embryos that hatch and the number of adults eclosing is dramatically reduced compared to controls.

      (Hughes and Krause, 2001)

      The severity of cuticle defects seen in eve1 embryos is increased if the mother is also heterozygous for groE48, groE73 or Df(3R)Espl22. The defects are pair rule in nature.

      (Kobayashi et al., 2001)

      Dominantly enhances the semi-lethality of trol13/Y males and shows a transheterozygous effect in trol13/+ females, resulting in a reduction in viability.

      (Park et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      eve5

      (Park et al., 1998)
      Partially rescued by

      eve1 is partially rescued by eveE+L.8.4

      (Fujioka et al., 1999)

      eve1 is partially rescued by eveE+L.9.2

      (Fujioka et al., 1999)
      Comments

      Cuticle of embryos rescued by single copy transgenes eveE+L.8.4 or eveE+L.9.2 show a high frequency of defects characteristic of hypomorphic eve mutants.

      (Fujioka et al., 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (7)
      Notes on Origin
      Discoverer

      Associated with: at least one recessive mutation that affects viability, as revealed in the unrescued condition of homozygotes, compared to hemizygotes, using eveE+L.8.4 or eveE+L.9.2.

      (Fujioka et al., 1999)
      Comments
      Comments

      prd RNA expression has been studied in odd5 eve1 double mutant embryos.

      (Baumgartner and Noll, 1990)

      A temperature-sensitive allele.

      (Yeo et al., 1995)

      The authors reference FBrf0042145 for "eve[ID19]". I couldn't find "eve[ID19]" nor "eve[1]" in the retrohack of FBrf0042145, but there is another allele, eve[3] curated from that paper. In FlyBase, "eve[ID19]" has been used many times as one of the synonyms for "eve[1]", but never for "eve[3]. I checked FBrf0042145, and indeed there are more alleles of "eve" mentioned in that paper in addition to "eve[R13]" (aka eve[3]). They say that one of these vaguely described alleles (which is clearly different from "eve[R13") is a temperature sensitive allele. This description matches the description of "eve[ID19]" in the present paper as being a temperature sensitive allele, so I think it is safe to assume that "eve[ID19]" refers to eve[1] here just like in all the other relevant papers in FlyBase. ra090930.

      (Leal et al., 2009)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      References (38)