FB2024_03 , released June 25, 2024
Allele: Dmel\E(z)32
Open Close
General Information
Symbol
Dmel\E(z)32
Species
D. melanogaster
Name
FlyBase ID
FBal0003411
Feature type
allele
Associated gene
Dmel\E(z)
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

heat sensitive loss of function allele

Mutagen
Nature of the Allele
Allele class

heat sensitive loss of function allele

(Phillips and Shearn, 1990)
Mutagen
ethyl methanesulfonate
(Phillips and Shearn, 1990)
Progenitor genotype
Cytology
Description

Amino acid replacement: C545Y. Nucleotide substitution: G1737A. Mutation lies within the Cys-rich region which spans amino acids 538-603.

(Carrington and Jones, 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:10,635,355..10,635,355 [-]
Nucleotide change:

G10635355A

Reported nucleotide change:

G1737A

Amino acid change:

C545Y | E(z)-PA; C545Y | E(z)-PB; C550Y | E(z)-PC

Reported amino acid change:

C545Y

(Carrington and Jones, 1996)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous females show no significant loss of fecundity when shifted to 29oC, however the viability of the eggs laid by these females drops from 39% to near zero within four hours of the temperature shift. The viability of eggs laid by homozygous females is restored to pre-temperature shift levels within one day after returning the females to the permissive temperature. After 8-12 hours of exposure of homozygous mothers to 30oC, a large proportion of homozygous larvae derived from these females show transformations of the thoracic and/or abdominal segments towards a denticle pattern resembling the eighth abdominal segment. Few, if any, heterozygous larvae derived from these females have transformations of denticle belt patterns.

      (Phillips and Shearn, 1990)

      Dominant suppressor of z mutants.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      E(z)32/E(z)61 has visible | homeotic phenotype, enhanceable by Sir2[+]/Sirt105327a

      (Furuyama et al., 2004)

      E(z)32/E(z)61 has visible | homeotic phenotype, enhanceable by Sirt1EP2300/Sir2[+]

      (Furuyama et al., 2004)
      Suppressor of
      Statement
      Reference

      E(z)[+]/E(z)32 is a suppressor of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

      (Morimoto et al., 2016)

      E(z)[+]/E(z)32 is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1UAS.cBa, Scer\GAL4Bx-MS1096

      (Morimoto et al., 2016)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      E(z)[+]/E(z)32 is a suppressor of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

      (Morimoto et al., 2016)

      E(z)[+]/E(z)32 is a suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1UAS.cBa, Scer\GAL4Bx-MS1096

      (Morimoto et al., 2016)

      E(z)32 is a suppressor of phenotype of z1

      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is ameliorated by combination with a single copy of E(z)32.

      (Morimoto et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference

      The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is ameliorated by combination with a single copy of E(z)32.

      (Morimoto et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Weak derepression of en expression.

      (Moazed and O'Farrell, 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      E(z)32
      (Morimoto et al., 2016)
      PC025
      (Kuzin et al., 1994)
      l(3)PC025
      pco25
      (Moazed and O'Farrell, 1992)
      pcopc025hs
      (Phillips and Shearn, 1990)
      pcopco25hs
      Name Synonyms
      Secondary FlyBase IDs
        References (9)