FlyBase Allele Report: Dmel\csw[5]

General Information

Symbol

Dmel\csw⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0001924

Feature type

allele

Associated gene

Dmel\csw

Associated Insertion(s)

Carried in Construct

Also Known As

csw^VA199

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Perkins et al., 1996, Perkins et al., 1992)

Mutagen

ethyl methanesulfonate

(Perkins et al., 1996, Perkins et al., 1992)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | embryonic stage | germline clone | rescuable maternal effect

(Perkins et al., 1996)

lethal | pupal stage | recessive

(Perkins et al., 1996)

lethal | recessive

(Perrimon et al., 1985)

lethal - all die before end of pupal stage

(Perkins et al., 1992)

some die during pupal stage

(Perkins et al., 1992)

Phenotype Manifest In

amnioproctodeal invagination

(Perkins et al., 1996)

arista

(Perkins et al., 1996)

embryonic/first instar larval cuticle | germline clone

(Perkins et al., 1996)

interommatidial bristle

(Perkins et al., 1996)

larval longitudinal connective | germline clone

(Perkins et al., 1996)

Malpighian tubule primordium

(Perkins et al., 1996)

mesoderm

(Johnson Hamlet and Perkins, 2001)

muscle cell of A1-7 dorsal acute muscle 1

(Johnson Hamlet and Perkins, 2001)

muscle cell of ventral acute muscle 2 | precursor

(Johnson Hamlet and Perkins, 2001)

neurogenic region

(Perkins et al., 1996)

ommatidium

(Perkins et al., 1996)

symmetrical commissure | germline clone

(Perkins et al., 1996)

tarsal segment

(Perkins et al., 1996)

tracheal precursor cell | germline clone

(Perkins et al., 1996)

unguis

(Perkins et al., 1996)

wing vein L5

(Perkins et al., 1996)

Detailed Description

Statement

Reference

Glial engulfment activity is normal in csw⁵ heterozygous flies.

(Logan et al., 2012)

Mutant embryos show a severe loss of eve-positive mesodermal cells. The DA1 muscle is never seen in these embryos later in development. csw⁵/Y embryos derived from homozygous female germline clones show loss of VA2 muscle precursor cells (only 29% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

csw⁵ germline clone mosaics cause maternal effect lethality. Embryos derived from mothers with germline clones, that exhibit a corkscrew cuticle phenotype (97%) have slight paternal rescue of the germline clone phenotype, embryos that do not receive a wild type copy from their father (3%), null embryos, die with a 'U'-shaped cuticle phenotype. In paternally rescued and null embryos specific terminal structures are entirely deleted and/or malformed. In mutant embryos ventral cells undergo cell fate changes to that of more lateral epidermal cell fates. Null embryos exhibit a severe CNS phenotype: horizontal commissures are collapsed. Paternally rescued embryos exhibit a less severe commissural phenotype. In both embryos the longitudinal tracts are rudimentary and discontinuous. Migration of tracheal precursor cells is defective, the mature trachea is barely recognisable as tracheal tissue. Pupal lethality can be rescued by heat induced expression of P{hs-SHP-2} during larval and pupal stages but not by expression of P{hs-SHP-1}.

(Perkins et al., 1996)

Abnormal development of terminal internal structures. Syncytial and cellular blastoderm embryos show reduced posterior tll domain. hb remains as a posterior cap and the seventh ftz stripe expands posteriorly, both due to lack of hkb repressing activity. In germline clones double mutant for phl^PB26 and csw⁵, tll is entirely missing posteriorly.

(Perkins et al., 1992)

Lethality occurs during larval and pupal stages. Phenotype of homozygous germ line clones is maternal effect lethal.

(Perrimon et al., 1985)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

csw⁵ has lethal | pupal stage phenotype, suppressible by Hsap\PTPN11^hs.PP

(Perkins et al., 1996)

NOT suppressed by

Statement

Reference

csw⁵ has lethal | pupal stage phenotype, non-suppressible by Hsap\PTPN6^hs.PP

(Perkins et al., 1996)

NOT Enhancer of

Statement

Reference

csw⁵ is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

csw⁵ is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

csw⁵ has muscle cell of A1-7 dorsal acute muscle 1 phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

csw⁵ has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

csw⁵ has mesoderm phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

NOT Enhancer of

Statement

Reference

csw⁵ is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

csw⁵ is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Other

Statement

Reference

Raf^PB26, csw⁵ has embryonic epidermis | posterior phenotype

(Ghiglione et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

A csw⁵ heterozygous mutant background completely suppresses the blocked severed axon debris engulfment seen in flies expressing drpr^II.Scer\UAS in glial cells under the control of Scer\GAL4^repo.

(Logan et al., 2012)

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in csw⁵/Y embryos derived from homozygous female germline clones. The eve-positive muscle progenitors and subsequent pericardial and DA1 precursor cells are recovered in csw⁵/Y embryos that are also expressing csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG.

(Johnson Hamlet and Perkins, 2001)

The extent to which posterior pattern elements are formed directly reflects the magnitude of tor signal. Thus mutants derived from germ line clones can be ordered with respect to increasing posterior abnormalities thus: phl¹ (normal posterior patterning) - csw⁶ - csw⁶,phl¹ - csw⁵,phl^PB26 (deletion of A8).

(Ghiglione et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

csw⁵ is rescued by csw^hs.PP

(Perkins et al., 1996)

Comments

Mutant phenotype can be rescued by csw^hs.PP. Fully zygotically rescued adult males are fertile and when crossed to heterozygous females produce both male and female progeny. These females generate eggs with fused dorsal appendages. Examination of rescued adults reveals consistent defects; absence of one or both aristae, lack of one or more of the distal-most leg segments, incomplete formation of the distal portions of wing vein L5, eyes have reduced numbers of disorganised ommatidia and interommatidial bristles.

(Perkins et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23874

csw⁵ P{FRT(w^hs)}101/FM7a

Notes on Origin

Discoverer

Lefevre.

Comments

Phenotypic series of csw mutant embryos derived from homozygous germline clones, classified according to cuticular phenotype: csw⁵ = csw^LE120 > csw¹ = csw^13-87 > csw^e13d.3 > csw⁷ = csw⁶.

(Perkins et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

VA199

(Perrimon et al., 1985)

csw⁵

(Popodi et al., 2010-)

csw^VA199

(Logan et al., 2012, Bach et al., 2003, Johnson Hamlet and Perkins, 2001, Ghiglione et al., 1999, Perkins et al., 1996, Perkins et al., 1992)

l(1)VA199

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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