FlyBase Allele Report: Dmel\ci[D]

General Information

Symbol

Dmel\ci^D

Species

D. melanogaster

Name

Dominant

FlyBase ID

FBal0001651

Feature type

allele

Associated gene

Dmel\ci

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

gain of function allele

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hidalgo and Ingham, 1990)

gain of function allele

(Akimaru et al., 1997, Von Ohlen et al., 1997, Locke and Tartof, 1994, Orenic et al., 1990)

loss of function allele

(Andrew et al., 1997)

Mutagen

X ray

Progenitor genotype

Caused by aberration

In(4)ci^D

Cytology

102A3 (determined by in situ hybridisation)

(Eaton and Kornberg, 1990)

Polytene chromosomes normal (Bridges).

Description

Rearrangement resulting in a fusion protein consisting of the first 246 amino acids of pan fused to ci coding sequences (the pan sequences are substituted for the first 12 amino acids of ci).

(Von Ohlen and Hooper, 1999)

In(4)ci^D is an inversion in which the promoter regions and first exons of pan and ci have been swapped. ci^D consists of a hybrid gene in which the pan promoter drives expression of a ci protein that carries the N-terminal region of pan. The predicted protein product consists of the first 246 amino acids of pan fused in frame to ci, of which the first 13 N-terminal amino acids are missing.

(Schweizer and Basler, 1998)

Small inversion, breakpoints are located 5' to the transcription unit and 10kb 3' to the transcription unit.

(Slusarski et al., 1995)

Two insertions of 4.9kb, one in the BglII HaeIII fragment and the second in the KpnI XhoI fragment.

(Locke and Tartof, 1994)

Small cytologically invisible inversion.

(Orenic et al., 1990)

ci¹, ci³⁶, ci⁵⁷, ci^D and ci^W mutant in the same 5.8 kb Bgl fragment. This fragment hybridizes to a 2.0 kb RNA that shows peak concentrations in late pupae.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Locke and Tartof, 1994, Hochman et al., 1964)

lethal - all die before end of pupal stage (with ciⁿ)

(Campbell and Tomlinson, 2000)

lethal - all die during embryonic stage

(Diaz-Benjumea et al., 1989)

lethal - all die during embryonic stage | recessive

(Eaton and Kornberg, 1990, Hochman, 1974)

some die during embryonic stage

(Diaz-Benjumea et al., 1989)

some die during embryonic stage | recessive

(Eaton and Kornberg, 1990, Hochman, 1974)

some die during pupal stage (with ciⁿ)

(Campbell and Tomlinson, 2000)

visible

(Hochman, 1974)

visible | adult stage

(Hatori and Kornberg, 2020)

visible | adult stage (with ci^mCherry)

(Hatori and Kornberg, 2020)

visible | dominant

(Vervoort et al., 1999, Schweizer and Basler, 1998, Akimaru et al., 1997, Locke and Hanna, 1996, Locke and Tartof, 1994, Diaz-Benjumea and Garcia-Bellido, 1990, Hochman et al., 1964)

visible | recessive

(Held, 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

ci^D/+ wings have a disrupted wing vein L4.

(Liu et al., 2011)

In heterozygotes, L4 is partially absent.

(Vervoort et al., 1999)

Wing vein L4 is truncated, and small amounts of ectopic wing vein are occasionally present. The posterior wing margin is fused to the alula, has larger bristles and is often nicked.

(Florence and McGinnis, 1998)

Heterozygotes show loss of wing margin bristles. This phenotype is dominantly enhanced by pan^S28.

(Schweizer and Basler, 1998)

Longitudinal wing vein 4 (L4) is shortened, some posterior row hairs are missing and the posterior wing margin is flattened in heterozygotes.

(Akimaru et al., 1997)

Double mutant phenotype with CrebA mutants is additive.

(Andrew et al., 1997)

ci^D/ci^W flies have abnormal legs.

(Sanchez-Herrero et al., 1996)

Naked cuticle is deleted and replaced with a mirror image duplication of the anterior denticle belt. ptc⁹; ci^D double mutants have features of both single mutants; naked cuticle is deleted and segment borders are duplicated.

(Motzny and Holmgren, 1995)

Keyhole structure of the larval foregut does not form.

(Pankratz and Hoch, 1995)

Coordinate mutant.

(Sturtevant and Bier, 1995)

Wing vein interruption.

(Locke and Tartof, 1994)

Frequent gaps in the midsection of row 1 of tarsal bristles, though the terminal bristles are always present.

(Held, 1993)

Mirror segmentation phenotype of embryos is not altered by wg^hs.PN.

(Sampedro et al., 1993)

Lack all longitudinal and transverse veins and affect morphogenesis of legs and eyes.

(Diaz-Benjumea and Garcia-Bellido, 1990)

Segment polarity phenotype.

(Eaton and Kornberg, 1990)

Dominant wing venation defect and recessive segmentation defect.

(Orenic et al., 1990)

Neuronal cell fate remains unchanged.

(Patel et al., 1989)

Interruptions in the fourth wing vein and slightly spread wings.

(Hochman et al., 1964)

Wings show interruptions of L4 in two places: proximal to and distal to anterior crossvein. L5 also shows distal interruption. L3 and L5 thick. Considerable plexus effect and knotting of veins. Wings broader, warped or concave upward, regularly extended and bent backward. Alula fused with and in same plane as blade of wing. Black dried haemolymph from axillary spiracle. Slight scalloping of inner wing margin, with hairs and tufts. Direction and extent of temperature effects depends on genetic background (Scharloo). In general, no overlapping of wild type. Inviable in combination with Ax/Ax or Ax/Y (House and Lutes, 1975). H/+ inhibits scalloping of ci^D but greatly enhances L4 interruption (House, 1959). Fully dominant in triplo-4's (Sturtevant, 1936). Two doses of ci^D reduce survival of triplo-4 flies (Parker, 1969). Homozygotes lethal in embryo (Hochman, 1971). Embryonic segment polarity disrupted; anterior portions of segments with their denticle belts duplicated in mirror-image fashion; posterior portions missing; each segment almost entirely covered with denticles (Nusslein-Volhard and Wieschaus, 1980). Fine hairs eliminated from dorsal abdominal segments; replaced with clear cuticle and socketed denticles (Orenic, Chidsey, and Holmgren, 1987). Embryonic CNS relatively normal (Patel et al., 1989). Genotype of oocyte with respect to ci^D without effect on phenotype of progeny (Orenic et al.). ci^D/l(4)102ABc dies as embryo, ci^D/l(4)102ABb as embryo or larva; in ci^D/ci^Ce-2 death usually delayed until pupal stage (Hochman, 1971). Survival of ci/ci^D/Df(4)M101-63a argues for nonallelism or pseudoallelism of ci and ci^D (Hochman, 1971). RK1.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ci^D has visible | dominant phenotype, enhanceable by Scer\GAL4^en-e16E/kn^UAS.cVa

(Vervoort et al., 1999)

Suppressed by

Statement

Reference

ci^D has visible | dominant phenotype, suppressible by nej[+]/nej³

(Akimaru et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

ci^D has wing vein L4 phenotype, enhanceable by Scer\GAL4^en-e16E/kn^UAS.cVa

(Vervoort et al., 1999)

ci^D has wing vein | ectopic phenotype, enhanceable by nej^Q7

(Florence and McGinnis, 1998)

Suppressed by

Statement

Reference

ci^D has wing margin phenotype, suppressible by nej³

(Florence and McGinnis, 1998)

ci^D has wing margin phenotype, suppressible by nej^Q7

(Florence and McGinnis, 1998)

ci^D has wing phenotype, suppressible by nej[+]/nej³

(Akimaru et al., 1997)

ci^D has trichome of the posterior wing margin phenotype, suppressible by nej[+]/nej³

(Akimaru et al., 1997)

NOT suppressed by

Statement

Reference

ci^W/ci^D has leg phenotype, non-suppressible by fu^J

(Sanchez-Herrero et al., 1996)

ci^W/ci^D has wing phenotype, non-suppressible by fu^J

(Sanchez-Herrero et al., 1996)

ci^D has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

ci^D/ci[+] is an enhancer of wing | ectopic phenotype of Antp^Ctx

(Prince et al., 2008)

ci^D/ci[+] is an enhancer of eye phenotype of Antp^Ctx

(Prince et al., 2008)

ci^D is an enhancer of phenotype of ptc^tuf-1

(Sanchez-Herrero et al., 1996)

Suppressor of

Statement

Reference

ci^D is a suppressor of phenotype of arm³

(Cavallo et al., 1998)

Other

Statement

Reference

Dd^G0269, ci^D has wing vein phenotype

(Liu et al., 2011)

Scer\GAL4^en-e16E, ci^D, kn^UAS.cVa has wing vein L5 phenotype

(Vervoort et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Dd^G0269/Y; ci^D/+ wings show a combination of the disrupted wing vein phenotype of ci^D/+ and the extra vein phenotype of Dd^G0269 in an additive manner.

(Liu et al., 2011)

Approximately 14% of Antp^Ctx/+;ci^D/+ flies exhibit ectopic wings on the head. Flies showing an ectopic wing on the head almost always show a normal or slightly reduced eye, whereas flies with a head capsule-to-thorax transformation mostly have reduced or lost eyes.

Ninety percent of the eyes of Antp^Ctx/+;ci^D/+ flies show a normal to weakly reduced eye phenotype.

(Prince et al., 2008)

When kn^Scer\UAS.cVa is driven by Scer\GAL4^en-e16E in a ci^D background, the truncation of L4 is stronger than seen in ci^D alone and L5 is partly missing.

(Vervoort et al., 1999)

The number of posterior row hairs is increased, and the wing is a normal shape in ci^D/+ nej³/+ transheterozygotes, although wing vein L4 is still shortened.

(Akimaru et al., 1997)

ci^D significantly increases the ptc^tuf-1 phenotype in ptc^tuf-1 ci^D/+ flies. The wing and leg phenotypes of ci^D/ci^W flies are not altered by fu^J.

(Sanchez-Herrero et al., 1996)

When doubly heterozygous with en loss of function alleles, the dominant ci phenotype is unchanged.

(Locke and Hanna, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

ci⁵⁷