FlyBase Allele Report: Dmel\bi[D4]

General Information

Symbol

Dmel\bi^D4

Species

D. melanogaster

Name

FlyBase ID

FBal0001124

Feature type

allele

Associated gene

Dmel\bi

Associated Insertion(s)

Carried in Construct

Also Known As

l(1)omb^D4, l(1)bi^D4, omb^D4

Key Links

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Kopp and Duncan, 1997)

Mutagen

X ray

(Poeck et al., 1993, Banga et al., 1986)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Banga et al., 1986)

Description

Point mutation causing premature termination of the protein.

(Tsai et al., 1997)

Lethal mutation is located within exon 2 around nucleotide position -38, 336bp from the lethal mutation of bi^omb-3198. At amino acid position 309, 5 nucleotides of the wild type sequence are substituted for 6 nucleotides producing a frameshift that results in a stop codon after 18 amino acids.

(Poeck et al., 1993)

Point mutation.

(Pflugfelder et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye disc | somatic clone | third instar larval stage | ventral

(Tsai et al., 2015)

eye disc | third instar larval stage | ventral (with bi^omb3-GAL4)

(Tsai et al., 2015)

wing | distal

(Grimm and Pflugfelder, 1996)

wing disc (with bi¹)

(Shen et al., 2008)

wing pouch (with bi¹)

(Shen et al., 2008)

wing vein L5 | cell non-autonomous | somatic clone

(Cook et al., 2004)

Detailed Description

Statement

Reference

In bi¹/bi^D4 larvae, overgrowth occurs in the anterior ventral pleura region of the wing disc and an ectopic furrow forms along the anteroposterior boundary of the wing pouch.

(Shen et al., 2008)

Somatic clones of bi^D4 homozygous cells that overlap wing vein L5 result in vein loss within the clone. This effect is not seen when these clones cross other veins, including L2.

(Cook et al., 2004)

Hemizygotes usually die at the late larval or early pupal stage, with a few animals surviving to the late pharate adult stage.

(Kopp and Duncan, 1997)

Analysis of mutant clones in the wings suggested that bi function is only required in the distal portion of the wing. Clones that reached the margin in the distal half of the wing blade produce severe scalloping, whereas internal clones, or clones that reached or crossed the margin proximally, were tolerated without phenotypic consequences.

(Grimm and Pflugfelder, 1996)

Displays lac and rb phenotypes.

(Pflugfelder et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

bi^D4 has lethal - all die before end of pupal stage | recessive phenotype, suppressible by Dp(1;3)DC116/+

(Liu et al., 2018)

Suppressor of

Statement

Reference

bi^D4 is a suppressor of decreased size | adult stage phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Tsai et al., 2015)

bi^D4 is a suppressor of decreased size | third instar larval stage phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Tsai et al., 2015)

bi^D4 is a suppressor of visible | adult stage phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Tsai et al., 2015)

Other

Statement

Reference

Dp(1;3)DC116/+, bi^D4 has viable phenotype

(Liu et al., 2018)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

bi^D4 has wing vein phenotype, non-enhanceable by brk^M68/brk^M68

(Cook et al., 2004)

NOT suppressed by

Statement

Reference

bi^D4 has wing vein phenotype, non-suppressible by brk^M68/brk^M68

(Cook et al., 2004)

Suppressor of

Statement

Reference

bi^D4 is a suppressor of eye phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Tsai et al., 2015)

bi^D4 is a suppressor of eye disc | third instar larval stage phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Tsai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

bi^D4, brk^M68 double homozygous somatic clones in the wing either contain no veins at all, or contain short fragments of ectopic vein that do not follow clone boundaries.

(Cook et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Stated in Lindsley and Zimm (1992) to be deleted for amb; but this is contrary to Banga et al. (1986) and has not been confirmed elsewhere.

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80151

y¹ w^* bi^D4/FM7a, y^* w^*; wg^Sp-1/CyO, Duox⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 5 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAB26698

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Df(1)bi-D4

bi^D4

(Liu et al., 2018)

l(1)bi^D4

(Kopp and Duncan, 1997, Tsai et al., 1997, Pflugfelder et al., 1990)

l(1)biD4

(Pflugfelder and Heisenberg, 1995)

l(1)omb^D4

(Wang et al., 2016, Tsai et al., 2015, Shen et al., 2008, Hazelett et al., 1998, Grimm and Pflugfelder, 1996)

omb^D4

(Cook et al., 2004, Grimm and Pflugfelder, 1996)

Name Synonyms

Secondary FlyBase IDs

FBab0000758

References (17)

Report Sections

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