FlyBase Allele Report: Dmel\ds[05142]

General Information

Symbol

Dmel\ds⁰⁵¹⁴²

Species

D. melanogaster

Name

FlyBase ID

FBal0000404

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

P{PZ}ds⁰⁵¹⁴²

Carried in Construct

Also Known As

ds-lacZ, ds-Z

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}ds⁰⁵¹⁴²

Cytology

Description

Insertion in the first intron.

(Merabet et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:701,895..701,895 [+]

(Okumura et al., 2015, Bosveld et al., 2012, Sagner et al., 2012, Matakatsu and Blair, 2004, Rodriguez, 2004, Campbell, 1999.3.29, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

costal vein | ectopic (with ds^38k)

(Rodriguez, 2004)

crossvein

(Matakatsu and Blair, 2006)

embryonic/larval brain | late third instar larval stage

(Gold and Brand, 2014)

embryonic/larval optic lobe | late third instar larval stage

(Gold and Brand, 2014)

humeral sclerite | ectopic (with ds^38k)

(Rodriguez, 2004)

leg (with ds¹)

(Rodriguez, 2004)

leg joint (with ds¹)

(Rodriguez, 2004)

optic lobe neuroepithelial cell | late third instar larval stage

(Gold and Brand, 2014)

scutellum | ectopic (with ds^38k)

(Rodriguez, 2004)

scutum | ectopic (with ds^38k)

(Rodriguez, 2004)

tarsal segment (with ds¹)

(Rodriguez, 2004)

tegula | ectopic (with ds^38k)

(Rodriguez, 2004)

wing

(Matakatsu and Blair, 2006)

wing (with ds^38k)

(Rodriguez, 2004)

wing | posterior (with ds^38k)

(Rodriguez, 2004)

wing blade (with ds^UAO71)

(Hogan et al., 2011)

wing disc

(Matakatsu and Blair, 2006)

wing hair

(Matakatsu and Blair, 2004)

wing hinge (with ds^38k)

(Rodriguez, 2004)

wing hinge | posterior (with ds^38k)

(Rodriguez, 2004)

wing margin | anterior | ectopic (with ds^38k)

(Rodriguez, 2004)

Detailed Description

Statement

Reference

No phenotypes are observed in ds⁰⁵¹⁴² mutant DL1 olfactory projection neurons.

(Okumura et al., 2015)

Neuroepithelial overproliferation results in grossly distorted brains with large optic lobes in ds⁰⁵¹⁴² late third instar larvae.

(Gold and Brand, 2014)

ds^UAO71/ds⁰⁵¹⁴² mutant wings display abnormal morphology. The region between wing veins L4 and L5 of ds^UAO71/ds⁰⁵¹⁴² mutant wings retains wild-type hair polarity, but has primarily anterior-posterior ridges.

(Hogan et al., 2011)

ds⁰⁵¹⁴² larvae show mild wing disc overgrowth.

ds⁰⁵¹⁴² adults have wings with widespread planar cell polarity defects and that are partially lacking the crossveins.

(Matakatsu and Blair, 2006)

ds⁰⁵¹⁴²/ds⁰⁵¹⁴² flies have severe planar polarity defects in their wings.

(Matakatsu and Blair, 2004)

Occasionally in ds^38k/ds⁰⁵¹⁴² escapers (approximately 2% of heminota), the wing is replaced by a winglet and, posterior to it, a lateral protuberance that looks like an scutum and scutellum. The winglet is composed of proximal anterior structures arranged in a mirror-image duplication. The smallest examples of these winglets consist of a mirror image duplication of anterior hinge structures - the tegula and humeral sclerite. Larger examples also have a rudimentary wing blade consisting of a mirror image duplication of anterior structures - the costa and anterior wing margin. ds⁰⁵¹⁴²/ds¹ flies have legs with reduced segment size and fusion of the tarsal segments and partial elimination of the tarsal joints.

(Rodriguez, 2004)

Escapers or pharate adults have a strong ds mutant phenotype.

(Couso, 2000.2.10)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

ds⁰⁵¹⁴² has abnormal planar polarity phenotype, non-enhanceable by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

NOT suppressed by

Statement

Reference

ds⁰⁵¹⁴² has abnormal planar polarity phenotype, non-suppressible by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

ds¹/ds⁰⁵¹⁴² has leg joint phenotype, enhanceable by dsh³/dsh[+]

(Rodriguez, 2004)

ds¹/ds⁰⁵¹⁴² has leg phenotype, enhanceable by dsh³/dsh[+]

(Rodriguez, 2004)

ds¹/ds⁰⁵¹⁴² has tarsal segment phenotype, enhanceable by dsh³/dsh[+]

(Rodriguez, 2004)

NOT Enhanced by

Statement

Reference

ds⁰⁵¹⁴² has wing hair phenotype, non-enhanceable by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

NOT suppressed by

Statement

Reference

ds⁰⁵¹⁴² has wing hair phenotype, non-suppressible by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Suppressor of

Statement

Reference

ds⁰⁵¹⁴²/ds⁰⁵¹⁴² is a suppressor | partially of wing hair | posterior phenotype of Scer\GAL4^en-e16E, fj^UAS.cZa

(Matakatsu and Blair, 2004)

ds⁰⁵¹⁴²/ds⁰⁵¹⁴² is a suppressor | partially of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, fj^UAS.cZa

(Matakatsu and Blair, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ds^Scer\UAS.cTa or ds^{ΔICD.Scer\UAS}, under the control of Scer\GAL4^αTub84B.PL, rescues the planar cell polarity defects of ds⁰⁵¹⁴² wings in all areas except the most proximal regions of the wing blade. Expression of either transgene driven by Scer\GAL4^ds-gal4 partially rescues planar cell polarity in the proximal wing but does not fully rescue the loss of the crossveins.

(Matakatsu and Blair, 2006)

The planar polarity phenotype of ds⁰⁵¹⁴²/ds⁰⁵¹⁴² wings is not enhanced by fj^d1/fj^d1.

(Matakatsu and Blair, 2004)

dsh³/+ enhances the severity of leg phenotypes in ds⁰⁵¹⁴²/ds¹ animals: The length of the segments is reduced and most of the tarsal joints very reduced or completely absent.

(Rodriguez, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ds⁵⁵

(Couso, 2000.2.10)

ds¹

(Couso, 2000.2.10)

Partially rescued by

ds⁰⁵¹⁴² is partially rescued by Scer\GAL4^ds-gal4/ds^UAS.cTa

(Matakatsu and Blair, 2006)

ds⁰⁵¹⁴² is partially rescued by ds^ΔICD.UAS/Scer\GAL4^ds-gal4

(Matakatsu and Blair, 2006)

ds⁰⁵¹⁴² is partially rescued by ds^UAS.cTa/Scer\GAL4^αTub84B.PL

(Matakatsu and Blair, 2006)

ds⁰⁵¹⁴² is partially rescued by ds^ΔICD.UAS/Scer\GAL4^αTub84B.PL

(Matakatsu and Blair, 2006)

ds⁰⁵¹⁴² is partially rescued by Scer\GAL4^αTub84B.PP/ds^UAS.cTa

(Matakatsu and Blair, 2004)

Not rescued by

ds⁰⁵¹⁴² is not rescued by ds^ΔECD.UAS/Scer\GAL4^ds-gal4

(Matakatsu and Blair, 2006)

Comments

Uniform ds^Scer\UAS.cTa expression using Scer\GAL4^αTub84B.PP rescues the planar cell polarity defects of ds⁰⁵¹⁴²/ds⁰⁵¹⁴² flies in the distal region between L3 and L4, but not in the proximal region just posterior to L1. Planar cell polarity defects in the posterior compartment of ft^Scer\UAS.cMa; Scer\GAL4^en-e16E flies are weakly suppressed by ds⁰⁵¹⁴²/ds⁰⁵¹⁴².

(Matakatsu and Blair, 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11394

P{PZ}ds⁰⁵¹⁴² cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

"FBti0004093 == FBti0000207 == P{PZ}ds⁰⁵¹⁴²" was stated as revision.

(Campbell, 1999.3.29)

Complements: ex⁰¹²⁷⁰. Complements: ex⁰²²⁷⁸. Complements: lwr⁰²⁸⁵⁸. Complements: Gsc⁰⁵³⁴¹. Complements: lwr⁰⁵⁴⁸⁶. Complements: α-Adaptin⁰⁶⁶⁹⁴. Complements: ex^k01108. Complements: l(2)k07005^k07005. Complements: l(2)k07308^k07308. Complements: l(2)k08915^k08915. Complements: ebi^k16213. Complements: RpI135^k16513.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

al⁰⁵¹⁴²

ds-Z

(Rodriguez, 2004)

ds⁰⁵¹⁴²

(Arata et al., 2017, Bosveld et al., 2016, González-Morales et al., 2015, Okumura et al., 2015, Gold and Brand, 2014, Matakatsu and Blair, 2012, Sagner et al., 2012, Hogan et al., 2011, Kawamori et al., 2011, Aigouy et al., 2010, Matakatsu and Blair, 2006)

ds^lacZ

(Kawamori et al., 2011)

l(2)05142

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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