FlyBase Allele Report: Dmel\βTub85D[8]

General Information

Symbol

Dmel\βTub85D⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0000029

Feature type

allele

Associated gene

Dmel\βTub85D

Associated Insertion(s)

Carried in Construct

Also Known As

B2t⁸

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Hutchens et al., 1997)

Mutagen

ethyl methanesulfonate

(Hoyle and Raff, 1990, Fuller et al., 1988)

Progenitor genotype

Cytology

Description

Amino acid replacement: E288K. Nucleotide substitution: GAG to AAG.

(Fackenthal et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,409,132..9,409,132 [-]

Nucleotide change:

G9409132A

Reported nucleotide change:

G?A

Amino acid change:

E288K | betaTub85D-PA

Reported amino acid change:

E288K

(Fackenthal et al., 1995, Hoyle and Raff, 1990, Fuller et al., 1988)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | male | recessive

(Fuller et al., 1988)

electrophoretic variant

(Fuller et al., 1988)

male fertile (with βTub85D⁶)

(Fuller, 1986)

male sterile (with βTub85Dⁿ)

(Fuller et al., 1988)

male sterile | recessive

(Hoyle and Raff, 1990)

male sterile | semidominant

(Fuller et al., 1988)

Phenotype Manifest In

axoneme

(Fuller et al., 1988)

meiotic cell cycle & spindle | male

(Fuller, 1986)

meiotic cell cycle | male

(Fuller, 1986)

spermatid

(Fuller et al., 1988, Fuller, 1986)

spermatid axoneme

(Fuller, 1986)

Detailed Description

Statement

Reference

Heterozygous males have significantly reduced fertility, although they do produce progeny. Chromosome segregation and cytokinesis during meiosis are abnormal in homozygous males. Chromosomes fail to move to the poles and remain scattered during meiosis I and II, although some separation of the chromosomes does occur. Early spermatids typically have multiple nuclei of various sizes, often associated with large mitochondrial derivatives. Nuclear shaping during spermatogenesis does not occur and nuclear alignment is defective. Clusters of abnormal microtubules are present in place of axonemes. In late spermatids these structures fill with the dense core material characteristic of wild-type central pair and accessory microtubules. Spermatid cyst elongation is very poor. Mitotic division before meiosis appears normal.

(Fuller et al., 1988)

Flagellar elongation is extremely defective in homozygous males. The developing spermatids contain a variety of aberrant microtubule structures, the most common of which are S-shaped microtubules. Closed microtubules and outer doublet-type structures are rarely seen. S-shaped microtubules are seen in both the astral and central spindle region of the meiotic spindle, in the place of axonemes in developing spermatid bundles and next to the nucleus during nuclear shaping. Meiosis is defective. Nuclear shaping is defective; although a few nuclei begin to flatten, most remain large and round.

(Fuller, 1986)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

βTub85D⁸ is a suppressor of male sterile phenotype of βTub60D::βTub85D^star

(Fackenthal et al., 1995)

Other

Statement

Reference

βTub60D::βTub85D^star, βTub85D⁸ has male fertile phenotype

(Fackenthal et al., 1995)

αTub84B^7.tHa, βTub85D⁸ has fertile | male | reduced phenotype

(Hays et al., 1989)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

βTub85D⁸ heterozygotes can fully rescue the male sterile phenotype of βTub60D::βTub85D^star; a large mass of motile sperm, equivalent to that in wild type males, is present in the seminal vesicles.

(Fackenthal et al., 1995)

Mutant males carrying one copy of αTub84B^7.tHa and heterozygous for βTub85D⁸ display reduced fertility in mating tests; the aberrant protein from βTub85D⁸ retains a residual function.

(Hays et al., 1989)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Encodes an electrophoretic variant protein that is shifted two charge units towards the basic direction compared to wild-type. The protein is stable and capable of forming an αβ tubulin heterodimer.

(Fuller et al., 1988)

Class II allele.

(Fackenthal et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

B2t⁸

(Hutchens et al., 1997, Fuller, 1993, Hoyle and Raff, 1990, Fuller et al., 1988)

Tβ2⁸

β2t⁸

(Fuller, 1986)

βTub85D⁸

Name Synonyms

Secondary FlyBase IDs

References (9)

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