FB2024_03 , released June 25, 2024
Allele: Dmel\βTub85D3
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General Information
Symbol
Dmel\βTub85D3
Species
D. melanogaster
Name
FlyBase ID
FBal0000025
Feature type
allele
Associated gene
Dmel\βTub85D
Associated Insertion(s)
Carried in Construct
Also Known As
B2t3
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Fackenthal et al., 1995)
Progenitor genotype
Cytology
Description

Amino acid replacement: S25L. Nucleotide substitution: TCG to TTG.

(Fackenthal et al., 1995)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:9,409,979..9,409,979 [-]
Nucleotide change:

C9409979T

Reported nucleotide change:

C?T

Amino acid change:

S25L | betaTub85D-PA

Reported amino acid change:

S25L

(Fackenthal et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The testes of homozygous males are characterised by complete lack of microtubule-dependent function in the postmitotic germ cells. There is also no evidence of meiotic spindle activity, the spermatid nuclei remain 4N and the spermatid head remains unshaped. The spermatid fails to elongate.

      (Fackenthal et al., 1995)

      Affects microtubule function during spermatogenesis.

      (Green et al., 1990)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      βTub85D3 has male sterile | dominant phenotype, enhanceable by wrl1

      (Green et al., 1990)
      Suppressor of
      Statement
      Reference

      βTub85D3 is a suppressor of male sterile phenotype of βTub60D::βTub85Dstar

      (Fackenthal et al., 1995)
      Other
      Statement
      Reference

      αTub84B7.tHa, βTub85D3 has fertile | male | reduced phenotype

      (Hays et al., 1989)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      βTub85D3 has phenotype, enhanceable by haync2

      (Fuller, 1986)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      βTub85D3 heterozygotes partially rescue the male sterile phenotype of males carrying βTub60D::βTub85Dstar; only a few motile sperm are present in the seminal vesicles.

      (Fackenthal et al., 1995)

      Mutant males carrying one copy of αTub84B7.tHa and heterozygous for βTub85D3 display reduced fertility in mating tests: the aberrant protein from βTub85D3 retains a residual function.

      (Hays et al., 1989)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Class I allele.

      (Fackenthal et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      B2t3
      (Fackenthal et al., 1995, Fuller et al., 1988, Regan and Fuller, 1988)
      Tβ23
      β2t3
      (Fuller, 1986)
      βTub85D3
      Name Synonyms
      Secondary FlyBase IDs
        References (8)