FB2024_03 , released June 25, 2024
Recombinant Construct: P{GMR71G01-lexA}
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General Information
Symbol
P{GMR71G01-lexA}
FlyBase ID
FBtp0079698
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lexAGMR71G01
Also Known As
R71G01-LexA
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lexAGMR71G01
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR71G01
Encoded product / tool
lexA::p65
Description

A 3844 base pair fragment from the flanking non-coding or intronic region of Vsx2 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
neuron | subset of adult brain
(Pan et al., 2012)
neuron | subset of adult ventral nerve cord
(Pan et al., 2012)
adult fruitless pMP-e (female) neuron | subset
(Pan et al., 2012)
adult doublesex pC1 (female) neuron
(Zhou et al., 2015)
adult doublesex pC1 (male) neuron
(Zhou et al., 2015)
adult fruitless P1 (male) neuron

Comment: A FLP/FRT strategy shows P{GMR71G01-lexA} drives expression in adult ingestion neuron 1 when combined with TI{GAL4::p65}CCKLR-17D3GAL4::p65.

(Wu et al., 2019)
adult stage | male
adult doublesex pC1 (male) neuron | subset
(Zhou et al., 2014)
adult fruitless P1 (male) neuron | subset
(Hoopfer et al., 2015)
Additional Information
Statement
Reference

A FLP/FRT intersectional strategy shows expression of EcollexAGMR71G01 in adult doublesex pC1 (female and male) neurons when combined with ScerGAL4dsx.Δ2.

(Zhou et al., 2015)

Expression driven by EcollexAGMR71G01 was restricted to a subset of adult fruitless pMP-e (male) neurons when combined with ScerGAL4dsx.Δ2 in a FLP based intersection strategy.

(Hoopfer et al., 2015)

EcollexAGMR71G01 drives expression in a subset of adult doublesex pC1 (male) neuron that also have expression driven by EcollexAfru.P1.

(Zhou et al., 2014)

EcollexAR71G01 drives expression in a subset of neurons in the adult CNS, including the male-specific P1 (pMP-e) neurons in the brain. The P1 neurons overlap with the expression of ScerGAL4fru.P1.B or ScerGAL4dsx-GAL4 in males. A small number of abdominal ganglion neurons and glial cells are also detected when expressing both EcollexAR71G01 and ScerGAL4dsx-GAL4.

(Pan et al., 2012)
 
Marker for
Reflects expression of
dsx
(Zhou et al., 2014)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
54733
w1118; P{GMR71G01-lexA}attP40
94687
w1118; P{GMR71G01-lexA}JK73A/TM3, Sb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (22)