FB2024_03 , released June 25, 2024
Recombinant Construct: P{W8,let7CΔmiR-125}
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General Information
Symbol
P{W8,let7CΔmiR-125}
FlyBase ID
FBtp0052055
Feature type
transgenic_transposable_element
Component Allele(s)
mir-100+tSa
mir-125Δ.cSa
mir-let7+tSa
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mW.hs
Component allele mir-let7+tSa
Transgenic product class
wild_type
(Sokol et al., 2008)
Component
Use(s)
Regulatory region(s)
mir-let7
Encoded product / tool
mir-let7
Description

Fragment that includes the entire let-7 microRNA.

(Sokol et al., 2008)
Component allele mir-100+tSa
Transgenic product class
wild_type
(Sokol et al., 2008)
Component
Use(s)
Regulatory region(s)
mir-100
Encoded product / tool
mir-100
Description

Fragment that includes the entire mir-100 microRNA.

(Sokol et al., 2008)
Component allele mir-125Δ.cSa
Transgenic product class
Component
Use(s)
Encoded product / tool
mir-125
Description

The mir-125 seed sequence (TCCCTGAGACCCTAA) has been deleted.

(Sokol et al., 2008)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

The mir-125 seed sequence (TCCCTGAGACCCTAA) has been deleted from the progenitor construct.

(Sokol et al., 2008)

The P{W8,let-7-CΔmiR-125} construct can rescue the male fertility and climbing defects of Df(2L)let-7-CKO1/Df(2L)let-7-CGKI adults. However, the rescued adults still show severely reduced locomotion and show partial defects in flight. Df(2L)let-7-CKO1/Df(2L)let-7-CGKI females rescued by P{W8,let-7-CΔmiR-125} show normal fertility.

(Sokol et al., 2008)

Df(2L)let-7-CKO1/Df(2L)let-7-CGKI adults rescued by the P{W8,let-7-CΔmiR-125} construct no longer show abnormal persistence of the dorsal internal oblique muscles (these muscles decay during post-eclosion maturation in wild-type animals) and no longer show defects in the dorsal muscles and their neuromuscular junctions.

(Sokol et al., 2008)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{W8,let-7-CΔmiR-125}
(FlyBase, 1996-)
P{W8,let7CΔmiR-125}
(FlyBase, 1996-)
P{v+, let-7-C ΔmiR-125}
(Pandey et al., 2021)
pP{W8,let-7-CΔmiR-125}
(Sokol et al., 2008, Sokol et al., 2008)
Secondary FlyBase IDs
    References (6)