FB2024_03 , released June 25, 2024
Recombinant Construct: P{ftz-lacB}
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General Information
Symbol
P{ftz-lacB}
FlyBase ID
FBtp0000175
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZftz.PB
Size
15.095
Expression Data
lacZ reporter
Associated insertion(s)
6 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZftz.PB
Transgenic product class
Component
Use(s)
Regulatory region(s)
ftz_lacB
ftz_neurogenic_enhancer
Encoded product / tool
lacZ
Description

lacZ reporter expression is driven by a deletion fragment of the ftz promoter that includes the zebra and neurogenic element.

(Hiromi et al., 1985)
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

15.095

Left end

P5\'

(Hiromi et al., 1985)
Right end

P3\'

(Hiromi et al., 1985)
 
Segments
Num
Orientation
Symbol
1
+
Car1_P5'(P5',R1)
2
+
EcoRI_site
3
-
001066
4
-
ftz::lacZ_6.8(Xb,u)
5
+
001065
6
+
Car20_P3'(H3,P3')
Reference
(Hiromi et al., 1985)
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 6 -- 17
organism | pair rule expression pattern
(Hiromi et al., 1985)
embryonic stage 11 -- 17
neuroblast | restricted
(Hiromi et al., 1985)
embryonic stage 13 -- 17
larval ventral nerve cord | segmentally repeated
(Hiromi et al., 1985)
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
epidermis | segmentally repeated
(Goriely et al., 1991)
larval ventral nerve cord | segmentally repeated
(Goriely et al., 1991)
embryonic stage 10 -- 11
mesoderm | pair rule expression pattern
(Hiromi and Gehring, 1987)
embryonic stage 15 -- 16
presumptive embryonic/larval central nervous system
(Hiromi and Gehring, 1987)
Additional Information
Statement
Reference

Expression in epidermis in a weak striped pattern.

(Goriely et al., 1991)

In germ-band extended embryos, strong expression in the mesoderm, but characteristic ftz expression in the ectoderm is greatly reduced. Expressed in even parasegments. In later embryos, expression is observed in the CNS.

(Hiromi and Gehring, 1987)

In gastrulating embryos, two weaker stripes observed anterior to the cephalic furrow (unlike wild-type ftz pattern). During mid-embryogenesis expression also observed in pairs of large cells; assumed to be neuroblasts. Expression throughout the ventral nerve cord (not in pair-rule pattern). Very weak epidermal expression.

(Hiromi et al., 1985)
 
Marker for
Reflects expression of
ftz
(Goriely et al., 1991, Hiromi and Gehring, 1987, Hiromi et al., 1985)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{ftz/lacB}
(Hiromi et al., 1985)
descendant(s)
Comments

construct_comment: Defined 3 regulatory regions: the zebra element, the neurogenic element, and the upstream element.

(Hiromi and Gehring, 1987)

report comment: Contains the ftz "zebra element" and "neurogenic element" enhancers.

(Hiromi and Gehring, 1987)

construct_comment: One of a series of lacZ reporter constructs designed to characterize 5\' regulatory elements of ftz.

(Hiromi et al., 1985)
Stocks (33)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P[ry,ftz/lacB]
(Hiromi et al., 1985)
P{ftz-lacB}
(FlyBase, 1996-)
P{ftz/lacB}
(FlyBase, 1996-, FlyBase, 1992-)
ftz/lacB
(Hiromi and Gehring, 1987)
Secondary FlyBase IDs
    References (7)