FB2024_03 , released June 25, 2024
Insertion: Dmel\PBac{5HPw+}NcaA502
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General Information
Symbol
Dmel\PBac{5HPw+}NcaA502
Species
D. melanogaster
Name
FlyBase ID
FBti0037705
Feature type
transposable_element_insertion_site
Description
Inserted Element
PBac{5HPw+}
LINE ID

PA08213

Affected gene(s)
CG7646, Gabat, Nca
Viability / fertility
fertile, viable
Associated allele(s)
NcaA502
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3L ( 76D8 )
Sequence Location
3L:19,994,823..19,994,823 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_PBac{5HPw+}.PA
Description

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{5HPw+}.

(Bellen et al., 2004)

PBac{5HPw+} is a 5' half P-element construct marked with the w+mC mini-white marker nested within a Tni\piggyBac-based construct; mobilization requires a source of Tni\piggyBac transposase.

(Ring and Garza, 2003.6.10)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:19,994,823..19,994,823 [-]
(Berkeley Drosophila Genome Project, 2006.4.26)
Orientation

-

(Berkeley Drosophila Genome Project, 2006.4.26)
Cytological location (computed by FlyBase)
76D8 (inferred by FlyBase from sequence location)
Cytological location (reported)
76D8 (reported as inferred from sequence location)
(Berkeley Drosophila Genome Project, 2006.4.26)
Insertion into Natural transposon
Comments concerning location

One of two insertions in the A502 line; see PBac{5HPw+}A502b. Alternatively, could be a single insertion associated with a 5-kb deletion.

(Berkeley Drosophila Genome Project, 2006.4.26)
Sequence Data
Inserted Element
Construct
PBac{5HPw+}
Size (Kb)

6.938

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

LINECOMMENT:Flanks map 5 kb apart consistent with two insertions or an insertion associated with a deletion. Junction at 5-prime end of vector is non-canonical, lacking both 5-prime end of vector and canonical TTAA target site duplication in flank.

(Berkeley Drosophila Genome Project, 2006.4.26)

Resequencing confirmed original insertion site.

(Ring and Garza, 2003.6.10)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{5HPw+}A502
(FlyBase, 1992-)
PBac{5HPw+}NcaA502
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (8)