FlyBase Insertion Report: Dmel\P{GawB}Kurs58

General Information

Symbol

Dmel\P{GawB}Kurs58

Species

D. melanogaster

Name

FlyBase ID

FBti0017957

Feature type

transposable_element_insertion_site

Also Known As

Kurs58, kurs58-GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

viable

Associated allele(s)

Scer\GAL4^Kurs58

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 28B2--28B3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

28B2--28B3 (in situ hybridization reported)

(Siegmund and Korge, 2001)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Crocker et al., 2010, Shahidullah et al., 2009, Pitman et al., 2006, Rodan et al., 2002, Siegmund and Korge, 2001)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^Kurs58

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval DMS cell of pars intercerebralis

(Siegmund and Korge, 2001)

larval Eh neurosecretory neuron

(Siegmund and Korge, 2001)

larval Hugin neuron of the corpus cardiacum

(Siegmund and Korge, 2001)

larval subesophageal capability neuron

(Siegmund and Korge, 2001)

adult stage

adult pars intercerebralis | restricted

(Cavanaugh et al., 2014)

adult ellipsoid body ring neuron R4

(Cavanaugh et al., 2014)

adult dorsal giant interneuron

(Rodan et al., 2002)

Additional Information

Statement

Reference

ScerGAL4^Kurs58 drives strong expression in around 16 to 18 cells of the adult pars intercerebralis (PI) that do not express Ilp2. In addition, expression is found in very few cells in the remaining adult cerebrum including ring neurons R4 of the ellipsoid body. GFP reconstitution across synaptic partners (GRASP) shows connections between ScerGAL4^Kurs58 positive PI-cells and DN1 neurons. The expression of ScerGAL4^Kurs58 in dendrites in combination with MmusIcam5^{Scer\UAS.T:DiscRFP-mCherry} shows close apposition of postsynaptic components of PI-cells with presynaptic proteins in DN1 neurons.

(Cavanaugh et al., 2014)

Marker for

Reflects expression of

Reporter construct used in assay

Ecol\lacZ^{UAS.Tag:MT(btau)}

(Siegmund and Korge, 2001)

Avic\GFP^{UAS.Tag:NLS(SV40-largeT),Tag:NES(oPKI)}

(Cavanaugh et al., 2014)

Mmus\Cd8a^UAS.cLa.EGFP

(Cavanaugh et al., 2014)

Mmus\Icam5^UAS.mCherry

(Cavanaugh et al., 2014)