FlyBase Insertion Report: Dmel\P{PZ}ari-2[07768]

General Information

Symbol

Dmel\P{PZ}ari-2⁰⁷⁷⁶⁸

Species

D. melanogaster

Name

FlyBase ID

FBti0005329

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)07768

Affected gene(s)

ari-2, Ecol\lacZ

Viability / fertility

Associated allele(s)

ari-2⁰⁷⁷⁶⁸, Ecol\lacZ^ari-2-07768

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 58D1 )

Sequence Location

2R:22,212,886..22,212,886 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:22,212,886..22,212,886 [+]

Orientation

Cytological location (computed by FlyBase)

58D1 (inferred by FlyBase from sequence location)

Cytological location (reported)

58D1-58D2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00612

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

ari-2

(Kuo et al., 2006, Treisman and Rubin, 1996)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, Treisman and Rubin, 1996, Meister and Braun, 1995.10)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

ari-2⁰⁷⁷⁶⁸

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^ari-2-07768

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval somatic muscle cell

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval central nervous system

(Jackle and Janning, 1998.8.10)

embryonic/larval brain | presumptive

(Jackle and Janning, 1998.8.10)

embryonic/larval anterior midgut

(Jackle and Janning, 1998.8.10)

embryonic/larval midgut | restricted

(Jackle and Janning, 1998.8.10)

embryonic/larval posterior midgut

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval peripheral nervous system | segmentally repeated

(Jackle and Janning, 1998.8.10)

mesoderm

(Jackle and Janning, 1998.8.10)

third instar larval stage

eye disc

(Treisman and Rubin, 1996)

eye disc posterior to the morphogenetic furrow

(Treisman and Rubin, 1996)

wandering third instar larval stage

gonad

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval brain

(Meister and Braun, 1995.10)

eye disc

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In embryos, expression in single cells in the midgut; expression in the somatic muscle is weak; expression in mesoderm is uncertain.

(Jackle and Janning, 1998.8.10)

Expressed in all cells of the eye disc, "starting soon after the morphogenetic furrow".

(Treisman and Rubin, 1996)

In wandering third instar, relatively weak β-gal staining in larval brain, imaginal discs, and gonad; strongest β-gal signal in the eye disc.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)07768

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

12341

P{PZ}ari-2⁰⁷⁷⁶⁸ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

07768

(Watts et al., 2003)

2-7768

(Treisman and Rubin, 1996)

P2341

(Meister and Braun, 1995.10)

P{PZ}ari-2⁰⁷⁷⁶⁸

(FlyBase, 1992-)

P{PZ}ari2⁰⁷⁷⁶⁸

(FlyBase, 1992-)

P{PZ}l(2)01738⁰⁷⁷⁶⁸

(Treisman and Rubin, 1996, FlyBase, 1992-)

P{PZ}l(2)07768⁰⁷⁷⁶⁸

(Treisman and Rubin, 1996, Meister and Braun, 1995.10, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)01738⁰⁷⁷⁶⁸

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000277
FBti0004262

References (13)

Report Sections

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