FlyBase Insertion Report: Dmel\P{PZ}TER94[03775]

General Information

Symbol

Dmel\P{PZ}TER94⁰³⁷⁷⁵

Species

D. melanogaster

Name

FlyBase ID

FBti0005249

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)03775

Affected gene(s)

Ecol\lacZ, TER94

Viability / fertility

Associated allele(s)

Ecol\lacZ^TER94-03775, TER94⁰³⁷⁷⁵

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 46D1 )

Sequence Location

2R:9,989,185..9,989,185 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:9,989,185..9,989,185 [-]

Orientation

Cytological location (computed by FlyBase)

46D1 (inferred by FlyBase from sequence location)

Cytological location (reported)

46D1-46D2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location reported as 2R:5876683..5876690 [+].

(Ruden et al., 2000, Leon and McKearin, 1999)

Sequence Data

Flanking sequence

AQ025617

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Vosshall, 1998.8.10, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

TER94

(Higashiyama et al., 2002, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^TER94-03775

Phenotypic Class Phenotype Manifest in Detailed Description

TER94⁰³⁷⁷⁵

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval brain

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In wandering third instar, weak β-gal staining in larval brain and imaginal discs. (Not specified which imaginal discs.)

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)03775

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11349

cn¹ P{PZ}TER94⁰³⁷⁷⁵/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:5500997-5500998 determined by FlyBase alignment of dbGSS accession AQ025617 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

The P element insertion is in the opposite orientation with respect to TER94 gene transcription.

(Ruden et al., 2000, Leon and McKearin, 1999)

insertion associated host repeat

(Ruden et al., 2000, Leon and McKearin, 1999)

P{lacW}TER94^k15502 and P{PZ}TER94⁰³⁷⁷⁵ are inserted at an identical sequence in the TER94 5' untranslated region.

(Ruden et al., 2000, Leon and McKearin, 1999)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1349

(Meister and Braun, 1995.10)

P{PZ}TER94⁰³⁷⁷⁵

(FlyBase, 2005, Ruden et al., 2000, Leon and McKearin, 1999, FlyBase, 1992-)

P{PZ}l(2)03775⁰³⁷⁷⁵

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)03775⁰³⁷⁷⁵

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000333

References (17)

Report Sections

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