FB2024_03 , released June 25, 2024
Insertion: Dmel\P{PZ}ken02970
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General Information
Symbol
Dmel\P{PZ}ken02970
Species
D. melanogaster
Name
FlyBase ID
FBti0005227
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(2)02970

Affected gene(s)
Ecol\lacZ, ken
Viability / fertility
Associated allele(s)
Ecol\lacZken-02970, ken02970
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 60A6 )
Sequence Location
2R:23,870,740..23,870,747 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:23,870,740..23,870,747 [+]
(Lukacsovich et al., 1999)
Orientation
Cytological location (computed by FlyBase)
60A6 (inferred by FlyBase from sequence location)
Cytological location (reported)
60A5-60A9 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location

Genomic location previously reported as 2R:19758258..19758258 [+].

(BDGP Project Members, 1994-1999)

Reported orientation for this insertion may be in error; based on alignment and description in flanking sequence accession, orientation is 3' to 5' (minus).

(FlyBase, 1996-)
Sequence Data
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval corpus allatum
(Harvie et al., 1998)
prothoracic gland
(Harvie et al., 1998)
wandering third instar larval stage
imaginal disc
(Harvie et al., 1998, Meister and Braun, 1995.10)
embryonic/larval optic lobe
(Harvie et al., 1998)
larval ventral nerve cord
(Harvie et al., 1998)
adult stage
adult brain | restricted
(Vosshall, 1998.8.10)
adult central complex | restricted
(Vosshall, 1998.8.10)
adult stage & oogenesis
follicle cell
• nucleus
(Liu and Montell, 1999)
Additional Information
Statement
Reference

During oogenesis, expressed uniformly in all follicle cell nuclei.

(Liu and Montell, 1999)

In the adult, low level of expression in discrete regions of the central brain.

(Vosshall, 1998.8.10)

During all larval stages, expressed in lateral secretory cells (prothoracic gland) and medial secretory cells (corpus allatum) of the ring gland.

(Harvie et al., 1998)

In wandering third instar larva, strong expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)02970

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

8bp insertion associated host repeat; The P element is inserted in a negative orientation with respect to ken gene transcription.

(Lukacsovich et al., 1999)
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
PZ2970
(Liu and Montell, 1999)
P{PZ}ken02970
(Yoon et al., 2011, Lukacsovich et al., 1999, FlyBase, 1992-)
P{PZ}l(2)0297002970
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}ken02970
(Sinenko et al., 2004, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000238
References (19)