FlyBase Insertion Report: Dmel\P{PZ}repo[03702]

General Information

Symbol

Dmel\P{PZ}repo⁰³⁷⁰²

Species

D. melanogaster

Name

FlyBase ID

FBti0003552

Feature type

transposable_element_insertion_site

Also Known As

repo-lacZ

Description

Inserted Element

P{PZ}

LINE ID

l(3)03702

Affected gene(s)

Ecol\lacZ, repo

Viability / fertility

Associated allele(s)

Ecol\lacZ^repo-03702, repo⁰³⁷⁰²

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 90F9 )

Sequence Location

3R:18,236,199..18,236,200 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:18,236,199..18,236,200 [+]

(Halter et al., 1995)

Orientation

Cytological location (computed by FlyBase)

90F9 (inferred by FlyBase from sequence location)

Cytological location (reported)

90F1-90F2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 3R:14061922..14062180 [+].

(BDGP Project Members, 1994-1999)

Reported orientation for this insertion may be in error; based on alignment and description in flanking sequence accession, orientation is 3' to 5' (minus).

(FlyBase, 1996-)

Sequence Data

Flanking sequence

AQ073316

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Spokony and Restifo, 2009, Tayler et al., 2004, Harvie et al., 1998, Vosshall, 1998.8.10, Meister and Braun, 1995.10, Xiong et al., 1994)

repo

(Xiong et al., 1994)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^repo-03702

Phenotypic Class Phenotype Manifest in Detailed Description

repo⁰³⁷⁰²

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic longitudinal glial cell

(Xiong et al., 1994)

exit glial cell

(Xiong et al., 1994)

PNS glial cell

(Xiong et al., 1994)

neuropil glial cell

(Xiong et al., 1994)

glial cell of central nervous system

(Xiong et al., 1994)

presumptive embryonic/larval peripheral nervous system

(Xiong et al., 1994)

third instar larval stage

glial cell of eye-antennal disc

(Xiong et al., 1994)

adult optic lobe

(Xiong et al., 1994)

wandering third instar larval stage

eye disc

(Harvie et al., 1998)

embryonic/larval brain

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

ring gland

(Harvie et al., 1998)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

medulla

(Xiong et al., 1994)

adult optic lobe

(Vosshall, 1998.8.10)

lamina

(Xiong et al., 1994)

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, expressed in the central brain, the optic lobe, and the third antennal segment.

(Vosshall, 1998.8.10)

Expression in the larval CNS exhibits a punctate pattern.

(Harvie et al., 1998)

In wandering third instar, weak β-gal staining in the larval brain.

(Meister and Braun, 1995.10)

In adults, enhancer trap expression is observed in glia of the laminal and medullar regions of the optic lobes. The laminar glia appear to correspond to the marginal glia, epithelial glia, satellite glia, and subretinal cells. In third instar larvae, expression is also restricted to glial cells and is observed in the eye disc and in a region that develops into the optic lobe in the larval brain. In embryos, enhancer trap expression was observed in many of the glia of the CNS and PNS but not in the midline glia. Expressing cells include the longitudinal glia, exit glia, peripheral glia, and nerve root glia.

(Xiong et al., 1994)

Marker for

Reflects expression of

repo

(Meister and Braun, 1995.10, Xiong et al., 1994)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)03702

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11604

ry⁵⁰⁶ P{PZ}repo⁰³⁷⁰²/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

The P insertion is in a negative orientation with repect to repo gene transcription.

(Halter et al., 1995)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P1604

(Meister and Braun, 1995.10)

P{PZ}l(3)03702⁰³⁷⁰²

(FlyBase, 1992-)

P{PZ}repo⁰³⁷⁰²

(Spokony and Restifo, 2009, Halter et al., 1995, Xiong et al., 1994, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)03702⁰³⁷⁰²

(FlyBase, 1992-)

P{ry^+t7.2=PZ}repo⁰³⁷⁰²

(FlyBase, 1992-)

repo-lacZ

(Spokony and Restifo, 2009, Tayler et al., 2004)

repo⁰³⁷⁰²

(Kerr et al., 2014)

Name Synonyms

Secondary FlyBase IDs

FBti0000543
FBti0003027

References (21)

Report Sections

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