FlyBase Insertion Report: Dmel\P{lacW}hkb[s5953]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Insertion: Dmel\P{lacW}hkb^s5953

General Information

Symbol

Dmel\P{lacW}hkb^s5953

Species

D. melanogaster

Name

FlyBase ID

FBti0002357

Feature type

transposable_element_insertion_site

Also Known As

hkb-lacZ, huckebein-lacZ, hkb⁵⁹⁵³-lacZ

Description

Inserted Element

LINE ID

Affected gene(s)

Viability / fertility

viable

Associated allele(s)

Ecol\lacZ^hkb-s5953, hkb^s5953

Stock availability

None publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

(FlyBase, 1992-)

Size (Kb)

10.691

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^hkb-s5953

Phenotypic Class Phenotype Manifest in Detailed Description

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

ventral neurectoderm

Comment: row 5

(Chu-LaGraff and Doe, 1993)

embryonic stage 10 -- 11

neuroblast NB2-2

(Doe, 1992)

embryonic stage 11

neuroblast NB2-4

(Doe, 1992)

neuroblast NB5-5

Comment: late stage 11

(Doe, 1992)

neuroblast NB5-4

(Doe, 1992)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuroblast of ventral nerve cord | subset

(Higashijima et al., 1996)

embryonic stage 9 -- 10

ventral neurectoderm

Comment: row 2/3

(Chu-LaGraff and Doe, 1993)

neuroblast NB2-3

(Chu-LaGraff and Doe, 1993)

embryonic stage 10 -- 11

neuroblast NB1-1

Comment: reference states 5-5.5 hr AEL

(Bhat et al., 2000)

neuroblast NB2-2

Comment: reference states 5-5.5 hr AEL

(Bhat et al., 2000)

neuroblast NB2-5

Comment: reference states 5-5.5 hr AEL

(Bhat et al., 2000)

neuroblast NB4-2

Comment: reference states 5-5.5 hr AEL

(Bhat et al., 2000)

embryonic stage 11

neuroblast NB4-4

(Doe, 1992)

neuroblast NB7-3

Comment: late stage 11

(Doe, 1992)

neuroblast NB4-3

Comment: late stage 11

(Doe, 1992)

neuroblast NB2-1

(Doe, 1992)

embryonic stage 11 -- 16

larval B-subperineurial glial cell

(Beckervordersandforth et al., 2008)

larval A-subperineurial glial cell

(Beckervordersandforth et al., 2008)

lateral ventral subperineurial glial cell

(Beckervordersandforth et al., 2008)

embryonic/larval neuron

(Beckervordersandforth et al., 2008)

embryonic ventral longitudinal glial cell

(Beckervordersandforth et al., 2008)

embryonic lateral longitudinal glial cell

(Beckervordersandforth et al., 2008)

embryonic stage 17

larval abdominal A-subperineurial glial cell | faint

(Bossing et al., 1996)

larval thoracic A-subperineurial glial cell | faint

(Bossing et al., 1996)

larval B-subperineurial glial cell

(Bossing et al., 1996)

larval RP2 motor neuron

(Bossing et al., 1996)

larval serotonergic VNC neuron

(Lundell et al., 1996)

first instar larval stage

larval abdominal ganglion Leucokinin neuron

(Benito-Sipos et al., 2010)

abdominal neuroblast NB5-3

(Estacio-Gómez et al., 2013)

abdominal neuroblast NB3-5

(Estacio-Gómez et al., 2013)

adult abdominal ganglion Leucokinin neuron

(Estacio-Gómez et al., 2013)

Additional Information

Statement

Reference

Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d6, d8, v1; deuterocerebrum - d1-10, d12, d13, v1-v8; protocerebrum - cd2, cv7

(Urbach and Technau, 2003)

, EcollacZ^hkb-5953 is expressed in the CNSin row 2/3 and in patches of cells in rows 4 and 5 in wild type embryos.In wg mutant embryos, there is selective loss of , EcollacZ^hkb-5953 expressionin row 4.

(Chu-LaGraff and Doe, 1993)

Enhancer trap expression is observed in a specified subset of neuroblasts in embryonic stages 9-11.

(Doe, 1992)

Marker for

(Urbach and Technau, 2003)

Dd10 neuroblast

(Urbach and Technau, 2003)

Dd11 neuroblast

(Urbach and Technau, 2003)

Dd12 neuroblast

(Urbach and Technau, 2003)

Dd13 neuroblast

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

Pcd2 neuroblast

(Urbach and Technau, 2003)

Pcv7 neuroblast

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Urbach and Technau, 2003)

(Higashijima et al., 1996)

neuroblast NB1-1

(Bhat et al., 2000)

neuroblast NB2-2

(Bhat et al., 2000)

neuroblast NB2-5

(Bhat et al., 2000)

neuroblast NB4-2

(Bhat et al., 2000)

Reflects expression of

(Benito-Sipos et al., 2010, Beckervordersandforth et al., 2008, Urbach and Technau, 2003, Bhat et al., 2000, Bossing et al., 1996, Lundell et al., 1996)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

5933

(Urbach and Technau, 2003)

5953

(Lundell et al., 1996)

5953-LacZ

(Higashijima et al., 1996)

Hkb-LacZ

(Tio et al., 2011)

P{lacW}hkb^s5953

P{lacZ-un4}hkb⁵⁹⁵³

(Baumgardt et al., 2007, FlyBase, 1992-)

hkb-lacZ

(Gabilondo et al., 2016, Urbach et al., 2016, Birkholz et al., 2013, Estacio-Gómez et al., 2013, Benito-Sipos et al., 2010, Losada-Pérez et al., 2010, Urbach and Technau, 2003)

hkb-lacZ⁵⁹⁵³

(Tio et al., 2011)

hkb⁵⁹⁵³-lacZ

(Rubio-Ferrera et al., 2023, Díaz-de-la-Peña et al., 2020, Herrero et al., 2014, Estacio-Gómez et al., 2013, Benito-Sipos et al., 2010)

hkblacZ

(Díaz-de-la-Peña et al., 2020)

huckebein-lacZ

(Birkholz et al., 2013, Losada-Pérez et al., 2010, Beckervordersandforth et al., 2008, De Graeve et al., 2004, Ragone et al., 2003, Urbach and Technau, 2003)

line 5953

(Andrew et al., 1994)

Name Synonyms

Secondary FlyBase IDs

References (37)

Report Sections