Thomson, L., Shah, H.P., Akinwotu Adewale, V., Beise, A., Bliayang, C., Cioch, Z., Craig, M., Crump, A., Durdan, M., Espinosa, M., Feda, K., Feist, J., Fragoso, A., Haro, G., Hoffman, B., Horne, P., Houha, N., Hounnou, S., Inman, A., Jakobsze, D., Juarez-Morales, Y., Khan, Y., Kohler, J., Lawlor, R., Lieser, B., Loitz, R., Martinez, E., Martinez, A., Martinez, M., Maza, B., Mendoza, B., Miller, S., Mngodo, H., O'Shea, S., Piane, S.N., Raivala, E., Ruger, S., Singer, A., Strand, J.E., Traylor, A., Wright, A., McCabe, S., Pandit, S.S., Bieser, K., Croonquist, P., Taylor, E.E., Wittke-Thompson, J., Kagey, J.D., Devergne, O. (2024). Genetic Mapping and Phenotypic Analysis of GstE14 [E.4.1] on Eye and Antennae Development in Drosophila melanogaster.  MicroPubl Biol 2024(): .

FBrf0259384

Research paper

Genetic screens are valuable for identifying novel genes involved in the regulation of developmental processes. To identify genes associated with cell growth regulation in Drosophila melanogaster , a mutagenesis screen was performed. Undergraduate students participating in Fly-CURE phenotypically characterized the E.4.1 mutant which is associated with rough eyes and antennae overgrowth. Following complementation analysis and subsequent genomic sequencing, E.4.1 was identified as a novel mutant allele of GstE14 , a gene involved in ecdysone biosynthesis important for the timing of developmental events. The abnormal eye and antenna phenotypes observed resulting from the loss of GstE14 suggest its role in tissue growth.

PMC11056011 (PMC) (EuropePMC)