[49F10-49F10];[49F13-49F13];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
49F10;49F13
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.RB3}BSC272 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of P{XP}d05649 to be at Release 3 genomic coordinate 8283844 on chromosome arm 2R. This corresponds to 49F10 on the Release 3 and 4 genome maps. The predicted position of PBac{RB}CG4712e00550 on the Release 4 map is 49F13. Consequently, the cytological breakpoints of Df(2R)BSC272 are predicted to be 49F10;49F13.