From: Kevin Cook <kcook@XXXX> To: FlyBase-Cambridge <flybase-cambridge@XXXX> Cc: John Roote <j.roote@XXXX> Subject: Cambridge mutations Date: Thu, 14 Aug 2008 16:59:09 -0400 ( 21:59 BST) The following information accompanied stocks donated to the Bloomington Stock Center by John Roote, University of Cambridge. ms(2)34Fe[Z0639], ms(2)35Bi[Z0778], gft[Z1089], ms(2)35Bj[Z1683] and l(2)34Dh[Z2321] are EMS-induced mutations from the screen described in Koundakjian et al., Genetics 167: 203-206, 2004 (FBrf0179036). ms(2)34Fe[Z0639] was male sterile in combination with ms(2)34Fe[1]. ms(2)35Bi[Z0778] was male sterile in combination with ms(2)35Bi[z0090]. gft[Z1089] was male sterile over gft[2]. ms(2)35Bj[Z1683] defines a new male sterile locus lying between l(2)35Bd and l(2)35Bg based on complementation with Df(2L)k08808-rv63, Df(2L)b84a3 and Df(2L)Pi1A1 and noncomplementation with Df(2L)fn31, Df(2L)b80k and Df(2L)TE35BC-24. Candidates genes are therefore GABA-B-R1, CG33310 and CG31832. l(2)34Dh[Z2321] defines a new locus mapping between kuz and l(2)34Db. The presence of a lethal locus called l(2)35Cc was inferred from the lethality associated with Df(2L)rd9 (see FBrf0111326). PBac{WH}f03621 (FBti0051348) was lethal in combination with Df(2L)rd9 and P{SUPor-P}CG15266[KG01622] (FBti0021701), but complemented alleles of rd. Consequently, CG15266 corresponds to l(2)35Cc and the two insertions should be renamed PBac{WH}l(2)35Cc[f03621] and P{SUPor-P}l(2)35Cc[KG01622]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
