Hemsley, M.J., Mazzotta, G.M., Mason, M., Dissel, S., Toppo, S., Pagano, M.A., Sandrelli, F., Meggio, F., Rosato, E., Costa, R., Tosatto, S.C. (2007). Linear motifs in the C-terminus of D. melanogaster cryptochrome.  Biochem. Biophys. Res. Commun. 355(2): 531--537.

FBrf0194310

Research paper

The C-terminus of cryptochrome (CRY) regulates light responses in Drosophila. These include the light-dependent binding of Drosophila dCRY to the clock proteins PERIOD and TIMELESS in a yeast two-hybrid system, which we proved to be a convenient and reliable readout of the behavior of dCRY in vivo. In this study, we present a combination of in silico analysis and experimental validation in yeast, to identify novel functional motifs in the C-terminal region of dCRY. Our results suggest that linear motifs are present in this small region, which is a likely hotspot for molecular interactions.