FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cry, per
FlyBase ID
FBig0000097983
Interaction Network
Interactions Browser links
cry network
per network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0109474-3.Y2H
Reference
Ceriani et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

fused to LexA DNA-binding domain

cry 
per 

PER

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cry 
residue D410
mutation disrupting interaction
aa 410

D410N (cry[b] allele), coordinates relative to cry-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ceriani et al., 1999)

Three-hybrid system: yeast LexA-BD/VP16-AD. Cells were transformed with LexA-cry, VP16-per and untagged tim.

(Ceriani et al., 1999)
FBrf0137204-1.Y2H
Reference
Rosato et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

fused to LexA DNA-binding domain

per 

PER

prey

fused to B42 activation domain

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain
sufficient binding region
aa 524-685

coordinates relative to per-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Rosato et al., 2001)

The cry-per interaction was not observed using full length per protein.

(Rosato et al., 2001)

Two-hybrid system: yeast LexA-BD/B42-AD

(Rosato et al., 2001)
FBrf0137204-4.coIP.WB
Reference
Rosato et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

HA tag

per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rosato et al., 2001)
FBrf0179150-2.coIP.WB
Reference
Busza et al., 2004
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

Myc tag

per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Busza et al., 2004)
FBrf0194310-1.Y2H
Reference
Hemsley et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

dCRY

bait

fused to LexA DNA-binding domain

cry 
cry 
cry 
per 

dPER

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cry 
C terminal domain
necessary binding region
aa 493-542
cry 
TRAF2 ligand motif and hypothetical phosphorylation site
mutation disrupting interaction
aa 526

S526A

cry 
PDZ binding segment EM4
mutation disrupting interaction
aa 530

S530P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hemsley et al., 2007)

Two-hybrid system: yeast LexA-BD/B42-AD, interaction depends on exposure to light.

(Hemsley et al., 2007)
FBrf0221205-2.Y2H
Reference
Ozturk et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

fused to LexA DNA-binding domain

per 

PER

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ozturk et al., 2013)

The cry-per interaction is only detected when yeast cells are grown in light.

(Ozturk et al., 2013)

Two-hybrid system: yeast LexA-BD/B42-AD

(Ozturk et al., 2013)

Positive control.

(Ozturk et al., 2013)
FBrf0227136-1.coIP.WB
Reference
Kumar et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait
cry 

cry

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Kumar et al., 2014)
FBrf0239698-5.Y2H
Reference
Schlichting et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

cry

bait

fused to lexA DNA-binding domain

per 

PER

prey

fused to B42 activation domain

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain
sufficient binding region
aa 233-685
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/B42-AD

(Schlichting et al., 2018)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Schlichting et al., 2018)

Positive control.

(Schlichting et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)