To: Kathy Matthews <matthewk@XXXX> Cc: e.ryderXXXX, Kevin Cook <kcookXXXX>, Rachel Drysdale (Genetics) <rd120@XXXX> From: John Roote <jr32@XXXX> Subject: Re: RS lines for Bloomington Date: Thu, 23 Sep 2004 13:21:48 \+0100 I have attached lists made by Ed of the GT lines, their coordinates and their nearest genes, and another listing the accession nos. Do let me know if this is OK. The lines are all on a common isogenic X, 2 and 3 background (but note, unfortunately NOT the same background as the Drosdel kit). They all carry w1118 and are homozygous, with these exceptions: GT-000008 lethal balanced over TM6B, Hu 20/20 Hu GT-000078 semi-lethal balanced over TM6B, Hu, CyO floating 18/22 Hu, 8/22 Cy non-Hu flies have smaller, darker eyes, disrupted wings, reduced bristles. GT-000294 lethal CyO and TM6B, Hu 28/29 Cy, 23/29 Hu GT-000526 semi-lethal balanced over TM6B, Hu 16/31 flies are Hu \---------------------------------------------------------------------------- Accession: AJ833932 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000008, clone library P{GT1} Within gene mapping: GT-000008 maps within CG8927 Accession: AJ833933 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000028, clone library P{GT1} Accession: AJ833934 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000078, clone library P{GT1} Accession: AJ833935 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000184, clone library P{GT1} Accession: AJ833936 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000196, clone library P{GT1} Within gene mapping: GT-000196 maps within CG12024 Accession: AJ833937 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000230, clone library P{GT1} Within gene mapping: GT-000230 maps within eIF4AIII (CG7483). Accession: AJ833938 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000270, clone library P{GT1} Accession: AJ833939 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000294, clone library P{GT1} Within gene mapping: GT-000294 maps within cdc2 (CG5363). Accession: AJ833940 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000304, clone library P{GT1} Accession: AJ833941 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000327, clone library P{GT1} Accession: AJ833942 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000344, clone library P{GT1} Accession: AJ833943 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000359, clone library P{GT1} Within gene mapping: GT-000359 maps within Rgl (CG8865). Accession: AJ833944 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000502, clone library P{GT1} Within gene mapping: GT-000502 maps within Gef64C (CG32239). Accession: AJ833945 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000504, clone library P{GT1} Accession: AJ833946 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000508, clone library P{GT1} Accession: AJ833947 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000526, clone library P{GT1} Accession: AJ833948 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000530, clone library P{GT1} Accession: AJ833949 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000558, clone library P{GT1} Within gene mapping: GT-000558 maps within CG3428 Accession: AJ833950 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000656, clone library P{GT1} Within gene mapping: GT-000656 maps within Sulf1 (CG6725). Accession: AJ833951 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000718, clone library P{GT1} Within gene mapping: GT-000718 maps within CG11170 Accession: AJ833952 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000741, clone library P{GT1} Within gene mapping: GT-000741 maps within CG11790 Accession: AJ833953 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-000966, clone library P{GT1} Within gene mapping: GT-000966 maps within CG8398 Accession: AJ833954 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-001020, clone library P{GT1} Within gene mapping: GT-001020 maps within CG11523 Accession: AJ833955 Description: Drosophila melanogaster flanking sequence of P element insertion P{GT1}GT-001092, clone library P{GT1} \---------------------------------------------------------------------------- 'GT-000008','3R','12010099','89B12','backward' 'GT-000028','2R','10279890','51E7','backward' 'GT-000078','3L','1569497','62A7','backward' 'GT-000184','3L','15253128','71C2','backward' 'GT-000196','3L','1837849','62B7','backward' 'GT-000230','3R','4139040','84F11','forward' 'GT-000270','3L','9317612','67A9','backward' 'GT-000294','2L','10378501','31D11','backward' 'GT-000304','2R','16675716','57F5','forward' 'GT-000327','3R','2358893','83F1','backward' 'GT-000344','2R','18280568','59D3','forward' 'GT-000359','3L','13865525','70C5','backward' 'GT-000502','3L','4715059','64B17','backward' 'GT-000504','2R','18314029','59D4','forward' 'GT-000508','3R','5913216','85F10','forward' 'GT-000526','3R','14989783','91F8','forward' 'GT-000530','2L','7429656','27F6','forward' 'GT-000558','3L','9445276','67B9','forward' 'GT-000656','3R','12139961','89B18','backward' 'GT-000718','2R','17226371','58C5','forward' 'GT-000741','3R','20864105','96B18','forward' 'GT-000966','3L','6573043','65C1','forward' 'GT-001020','3L','22183932','79D3','backward' 'GT-001092','3L','8431575','66D1','backward' '\N','GT-000008','CG8925','proximal','4103' '\N','GT-000008','CG8927','in_gene','0' '\N','GT-000008','CG6046','distal','1799' '\N','GT-000008','CG6072','distal','2575' '\N','GT-000028','CG8092','proximal','2533' '\N','GT-000028','CG8093','proximal','565' '\N','GT-000028','CG11808','distal','44' '\N','GT-000028','CG12954','distal','857' '\N','GT-000028','CG8079','distal','2216' '\N','GT-000028','CG8094','distal','2670' '\N','GT-000078','CG13917','distal','4394' '\N','GT-000184','CG7259','proximal','3357' '\N','GT-000184','CG12327','proximal','1509' '\N','GT-000184','CG7255','distal','120' '\N','GT-000196','CG13936','proximal','4980' '\N','GT-000196','CG12024','in_gene','0' '\N','GT-000196','CG1956','distal','1894' '\N','GT-000230','CG9615','proximal','4681' '\N','GT-000230','CG11693','proximal','3369' '\N','GT-000230','CG11694','proximal','1218' '\N','GT-000230','CG11698','proximal','206' '\N','GT-000230','CG7483','in_gene','0' '\N','GT-000230','CG9613','distal','1481' '\N','GT-000230','CG7459','distal','4315' '\N','GT-000270','CG32040','proximal','3564' '\N','GT-000270','CG4347','proximal','51' '\N','GT-000270','CG32039','distal','121' '\N','GT-000270','CG4446','distal','1410' '\N','GT-000270','CG10923','distal','3729' '\N','GT-000294','CG5091','proximal','3857' '\N','GT-000294','CG5096','proximal','1362' '\N','GT-000294','CG5363','in_gene','0' '\N','GT-000294','CG5108','distal','572' '\N','GT-000294','CG5102','distal','1986' '\N','GT-000304','CG10080','proximal','904' '\N','GT-000304','CG15674','distal','121' '\N','GT-000304','CG10321','distal','2503' '\N','GT-000327','CG15185','proximal','4613' '\N','GT-000327','CG15183','proximal','109' '\N','GT-000344','CG3092','proximal','3072' '\N','GT-000359','CG8865','in_gene','0' '\N','GT-000502','CG32239','in_gene','0' '\N','GT-000504','CG12782','proximal','4927' '\N','GT-000504','CG13540','distal','123' '\N','GT-000504','CG3134','distal','794' '\N','GT-000526','CG5637','proximal','3842' '\N','GT-000526','CG11779','proximal','4166' '\N','GT-000526','CG5558','proximal','1766' '\N','GT-000526','CG5557','distal','177' '\N','GT-000530','CG5958','proximal','3945' '\N','GT-000530','CG5973','distal','81' '\N','GT-000558','CG3654','proximal','1215' '\N','GT-000558','CG3428','in_gene','0' '\N','GT-000558','CG32036','distal','391' '\N','GT-000558','CG32037','distal','2051' '\N','GT-000656','CG6725','in_gene','0' '\N','GT-000718','CG30279','proximal','3292' '\N','GT-000718','CG11275','proximal','170' '\N','GT-000718','CG11170','in_gene','0' '\N','GT-000718','CG5465','distal','326' '\N','GT-000718','CG5625','distal','3158' '\N','GT-000741','CG31107','proximal','4952' '\N','GT-000741','CG31110','proximal','2591' '\N','GT-000741','CG11786','proximal','192' '\N','GT-000741','CG11790','in_gene','0' '\N','GT-000741','CG11791','distal','2066' '\N','GT-000966','CG8368','proximal','4754' '\N','GT-000966','CG8398','in_gene','0' '\N','GT-001020','CG9063','proximal','3759' '\N','GT-001020','CG9054','proximal','636' '\N','GT-001020','CG11523','in_gene','0' '\N','GT-001020','CG6395','distal','607' '\N','GT-001092','CG6964','proximal','196' '\N','GT-001092','CG6915','distal','142' \----------------------------------------------------------------------------