From kcook@XXXX Fri Jul 04 02:45:43 2003 To: flybase-updates@XXXX Subject: Isolation and Characterization of Df(3R)BSC38 Isolation and Characterization of Df(3R)BSC38 Rachel Andrade, Jennifer Deal and Kevin Cook Bloomington Drosophila Stock Center, Indiana University Df(3R)BSC38 was isolated as a P transposase-induced male recombination event involving P{EP}EP3681 and P{EP}EP3340EP3340. The deletion was isolated as a st1-ca1 recombinant chromosome from the cross st1 Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 P{EP}EP3681/P{EP}EP3340EP3340 ca1 males. Polytene chromosome squashes showed the breakpoints 85F1-2;86C7-8 (i.e. breaks within both doublet bands to form a fusion band). Df(3R)BSC38 failed to complement P{PZ}tws02414, P{PZ}jumu06439 and hth5E04. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX