From rd120@XXXX Tue Mar 10 12:04:32 1998 To: mckim@XXXX Subject: ADRC: 802C Dear Hao and Kim, I am writing in connection with your abstract for the upcoming Washington DC ADRC: 'mei-P22 encodes a novel protein required for the initiation of meiotic recombination.' You mention mapping two genes, mei-P22 and mei-W68 with P insertions. Could you tell us the symbols for the P insertions in both cases? That way we can capture the mapping data rigorously for FlyBase. It is nice if we can keep as many gene records anchored to the map as possible. Thank you for your help, with best wishes, Rachel. PS: Hao's email address is not in FlyBase, so I'm sending it to both of you at Kim's address. From: 'Kim S. McKim' <mckim@XXXX> To: Rachel Drysdale (Genetics) <rd120@XXXX> Subject: Re: ADRC: 802C Dear Rachel, with regards to your inquiry: > You mention mapping two genes, mei-P22 and mei-W68 with P insertions. > Could you tell us the symbols for the P insertions in both cases? mei-P22 is not mapped relative to any P insertions, it is itself a P insertion mutation mapping to 65E. We have also genetically mapped it between ru and h, at approximately map position 21.5. mei-W68 on the other hand was mapped relative to P insertion mutations (lethals from the genome project). The genetic order is as follows: l(2)k09810 [l(2)01103, l(2)k00705, l(2)k16914] mei-W68 l(2)k06323 [l(2)05338, emm]. The genes in brackets are not ordered relative to themselves. Some of this data is based on physical rather than genetic data. For example, the insertions 1103, k705, k16914 and k6323 are all on the P1 clones DS571 and DS7982. The rest of the insertions are not anywhere on the contig (DS00433) It has been reported that l(2)05338 is allelic to sm. Hope this covers the situation. Truly yours, Kim Kim S. McKim Waksman Institute Rutgers University 190 Frelinghuysen Rd. Piscataway NJ 08854-8020 908 445-1164