FB2024_04 , released June 25, 2024
Gene: Dmel\ru
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General Information
Symbol
Dmel\ru
Species
D. melanogaster
Name
roughoid
Annotation Symbol
CG1214
Feature Type
protein_coding_gene
FlyBase ID
FBgn0003295
Gene Model Status
Current
Stock Availability
247 publicly available
Gene Summary
roughoid (ru) encodes an intra-membrane serine protease localized in the endoplasmic reticulum. It processes Egfr ligands in specific tissues, most notably the eye. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

rhomboid-3, rho-3, rho3, rhomboid 3, rhomboid3

Key Links
Genomic Location
Cytogenetic map
61F8-61F8
Sequence location
Recombination map
3-0
RefSeq locus
NT_037436 REGION:1370628..1387437
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables serine-type endopeptidase activity
inferred from direct assay
(Yogev et al., 2008, Tsruya et al., 2007, Urban et al., 2002)
inferred from mutant phenotype
(Yogev et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables serine-type endopeptidase activity
inferred from biological aspect of ancestor with PANTHER:PTN000533014
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR017213, InterPro:IPR022764
(InterPro Project Members, 2004-)
Biological Process (9 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
involved_in compound eye cone cell fate commitment
inferred from mutant phenotype
(Wasserman et al., 2000)
involved_in compound eye morphogenesis
inferred from mutant phenotype
(Yogev et al., 2010)
involved_in compound eye photoreceptor fate commitment
inferred from genetic interaction with FLYBASE:rho; FB:FBgn0004635
(Wasserman et al., 2000)
involved_in epidermal growth factor receptor ligand maturation
inferred from direct assay
(Yogev et al., 2008, Tsruya et al., 2007, Urban et al., 2002)
inferred from genetic interaction with FLYBASE:rasp; FB:FBgn0024194
(Steinhauer et al., 2013)
inferred from mutant phenotype
(Yogev et al., 2010)
involved_in epithelial cell migration, open tracheal system
inferred from mutant phenotype
(Gallio et al., 2004)
involved_in heart process
inferred from mutant phenotype
(Yu et al., 2010)
involved_in positive regulation of protein secretion
inferred from genetic interaction with FLYBASE:spi; FB:FBgn0005672
(Yogev et al., 2010)
involved_in protein processing
inferred from direct assay
(Yogev et al., 2008, Tsruya et al., 2007, Urban et al., 2002)
inferred from mutant phenotype
(Yogev et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in leg disc proximal/distal pattern formation
traceable author statement
(Mann and Casares, 2002)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in apical part of cell
inferred from direct assay
(Yogev et al., 2010)
located_in endoplasmic reticulum
inferred from direct assay
(Yogev et al., 2010)
located_in Golgi membrane
inferred from direct assay
(Urban et al., 2002)
located_in late endosome membrane
inferred from direct assay
(Tsruya et al., 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in membrane
inferred from electronic annotation with InterPro:IPR017213, InterPro:IPR022764
(InterPro Project Members, 2004-)
located_in plasma membrane
inferred from sequence or structural similarity with FLYBASE:rho; FB:FBgn0004635
(Wasserman et al., 2000)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
roughoid (ru) encodes an intra-membrane serine protease localized in the endoplasmic reticulum. It processes Egfr ligands in specific tissues, most notably the eye. [Date last reviewed: 2019-03-14]
Pathway (FlyBase)
EGFR Ligand Production -
Factors required for the proteolytic cleavage and/or secretion of transforming growth factor-α-like Egfr ligands spi, Krn or grk.
Gene Group (FlyBase)
RHOMBOID PROTEASES -
Rhomboid proteases are intramembrane serine proteases related to rho, that cleave other transmembrane proteins within their transmembrane domains. Rhomboid proteases are best characterized for their role in epidermal growth factor signaling, where they are required for the production the soluble, secreted ligand. They belong to MEROPS family S54. (Adapted from FBrf0235672).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ru: roughoid
Eyes small and rough, have irregular facets and hairs, and have black specks from erupted facets. Expression variable; sometimes overlaps wild type. SEM study by Stumm-Tegethoff and Dick (1974, Theor. Appl. Genet. 44: 262-65).
Summary (Interactive Fly)

intra-membrane serine protease localized in the endoplasmic reticulum - Rhomboid and Roughoid/Rhomboid-3 act together on an unknown Egf to control cell recruitment through the Epidermal growth factor receptor most notably the eye

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ru for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W0F8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ru-RA
FBtr0072681
NM_080051
1798
341
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
ru-PA
FBpp0072574
37.0
341
8.03
Q9W0F8
NP_524790
AAF47490
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ru using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.71

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
ventral sensory complex primordium
(Fisher et al., 2012)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
organism
(Yu et al., 2010)
adult stage
adult heart
(Yu et al., 2010)
leg
(Yu et al., 2010)
adult head
(Yu et al., 2010)
adult thorax
(Yu et al., 2010)
adult abdomen
(Yu et al., 2010)
Additional Descriptive Data

ru transcript is expressed at highest levels during pupal stages. In adult tissues, the highest levels of ru transcript expression are observed in the head and leg, with lowest expression levels observed in the heart.

(Yu et al., 2010)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in apical part of cell
inferred from direct assay
(Yogev et al., 2010)
located_in endoplasmic reticulum
inferred from direct assay
(Yogev et al., 2010)
located_in Golgi membrane
inferred from direct assay
(Urban et al., 2002)
located_in late endosome membrane
inferred from direct assay
(Tsruya et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ru in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ru
Transgenic constructs containing regulatory region of ru
Aberrations (Deficiencies and Duplications) ( 13 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Tub.PU
lethal, with Scer\GAL4Ubi.PU
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal cell polarity
abnormal neuroanatomy | third instar larval stage
abnormal neurophysiology | third instar larval stage
abnormal planar polarity | recessive
increased cell number | pupal stage
visible
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4CY2
visible, with Scer\GAL4GMR.PF
visible | recessive
wild-type
Phenotype manifest in
Allele
abdominal tergite 6 | female, with Scer\GAL4pnr-MD237
adult heart chamber
cone cell
cone cell | ectopic, with Scer\GAL4GMR.PF
egg, with Scer\GAL4CY2
embryonic/larval ganglionic tracheal branch
embryonic/larval ganglionic tracheal branch 1 | embryonic stage
embryonic/larval ganglionic tracheal branch (with Df(3L)Ar14-8)
eye
eye, with Scer\GAL4GMR.PF
eye photoreceptor cell
eye photoreceptor cell | ectopic, with Scer\GAL4GMR.PF
ommatidium
optic cartridge | third instar larval stage
photoreceptor
pigment cell
pigment cell | ectopic, with Scer\GAL4GMR.PF
retina | pupal stage, with Scer\GAL4GMR.PU
wing, with Scer\GAL4Bx-MS1096
wing vein | ectopic, with Scer\GAL4Bx-MS1096
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (7)
Hsap\RHBDL3
8 of 14
Yes
No
Hsap\RHBDL1
7 of 14
No
No
1  
Hsap\RHBDF1
4 of 14
No
No
1  
Hsap\RHBDF2
4 of 14
No
No
Hsap\RHBDL2
4 of 14
No
No
Hsap\PARL
1 of 14
No
No
Hsap\SLC25A24
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Rhbdl3
8 of 14
Yes
No
Rnor\Rhbdl1
5 of 14
No
Yes
Rnor\Rhbdf1
4 of 14
No
No
Rnor\Rhbdf2
4 of 14
No
No
Rnor\Rhbdl2
4 of 14
No
No
Rnor\Slc25a24
1 of 14
No
No
Mus musculus (laboratory mouse) (7)
Mmus\Rhbdl3
8 of 14
Yes
No
Mmus\Rhbdl1
7 of 14
No
No
Mmus\Rhbdf1
4 of 14
No
No
Mmus\Rhbdf2
4 of 14
No
No
Mmus\Rhbdl2
4 of 14
No
No
Mmus\Parl
1 of 14
No
No
Mmus\Slc25a24
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\rhbdf2
3 of 13
Yes
No
Xtro\rhbdl2
3 of 13
Yes
No
Xtro\rhbdl3
3 of 13
Yes
No
Xtro\rhbdf1
2 of 13
No
No
Xtro\rhbdl1
2 of 13
No
No
Xtro\slc25a24
1 of 13
No
No
Xtro\tmod1
1 of 13
No
No
Danio rerio (Zebrafish) (9)
Drer\rhbdl1
7 of 14
Yes
No
Drer\rhbdl3
7 of 14
Yes
No
Drer\rhbdl2
5 of 14
No
No
Drer\rhbdf1a
4 of 14
No
No
Drer\rhbdf1b
3 of 14
No
No
Drer\parla
1 of 14
No
No
Drer\parl
1 of 14
No
No
Drer\plch1
1 of 14
No
No
Drer\slc25a23a
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\rom-1
8 of 14
Yes
No
Cele\rom-2
6 of 14
No
No
Cele\rom-3
4 of 14
No
No
Cele\rom-4
3 of 14
No
No
Cele\rom-5
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (5)
Agam\AgaP_AGAP004737
6 of 12
Yes
No
Agam\AgaP_AGAP005058
5 of 12
No
No
Agam\AgaP_AGAP009451
3 of 12
No
No
Agam\AgaP_AGAP013190
3 of 12
No
No
Agam\AgaP_AGAP002626
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (16)
Atha\KOM
6 of 13
Yes
Yes
Atha\RBL1
6 of 13
Yes
Yes
Atha\RBL2
6 of 13
Yes
Yes
Atha\RBL3
6 of 13
Yes
Yes
Atha\RBL4
6 of 13
Yes
Yes
Atha\RBL5
6 of 13
Yes
Yes
Atha\RBL6
6 of 13
Yes
Yes
Atha\RBL7
6 of 13
Yes
Yes
Atha\AT5G38510
3 of 13
No
Yes
Atha\RBL10
3 of 13
No
Yes
Atha\AT1G74130
1 of 13
No
No
Atha\AT1G74140
1 of 13
No
No
Atha\AT5G25640
1 of 13
No
No
Atha\RBL12
1 of 13
No
No
Atha\RBL14
1 of 13
No
Yes
Atha\RBL15
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\PCP1
2 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\SPBC13E7.11
1 of 12
Yes
No
Spom\SPBP4H10.10
1 of 12
Yes
No
Escherichia coli (enterobacterium) (1)
Ecol\glpG
2 of 11
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:ru. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (6)
rho
10 of 13
rho-6
9 of 13
rho-4
8 of 13
stet
8 of 13
rho-5
6 of 13
rho-7
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
RHBDL3; rhomboid like 3
8 of 14
619017
      RHBDL1; rhomboid like 1
      7 of 14
      603264
           
          RHBDF1; rhomboid 5 homolog 1
          4 of 14
          614403
               
              RHBDF2; rhomboid 5 homolog 2
              4 of 14
              614404
              RHBDL2; rhomboid like 2
              4 of 14
              608962
                  Functional Complementation Data
                  Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                  Interactions
                  Summary of Physical Interactions
                  esyN Network Diagram
                  Show neighbor-neighbor interactions:
                  Show/hide secondary interactors 
                  (data from AllianceMine provided by esyN)
                  Select Layout:
                  Legend:
                  Protein
                  RNA
                  Selected Interactor(s)
                  Other Interaction Browsers
                  View in FlyBase Interactions Browser   View in MIST tool (external link)
                  Please see the Physical Interaction reports below for full details
                  protein-protein
                  Physical Interaction
                  Assay
                  References
                  ru - Krn
                  inferred by author, enzymatic study, anti tag western blot
                  (Urban et al., 2002)
                  ru - grk
                  inferred by author, enzymatic study, anti tag western blot
                  (Urban et al., 2002)
                  ru - spi
                  inferred by author, enzymatic study, anti tag western blot
                  (Urban and Freeman, 2003, Urban et al., 2002)
                  Summary of Genetic Interactions
                  esyN Network Diagram
                  Show/hide secondary interactors 
                  (data from AllianceMine provided by esyN)
                  esyN Network Key:
                  Suppression
                  Enhancement
                  Other Interaction Browsers
                  View in FlyBase Interactions Browser   View in MIST tool (external link)
                  Please look at the allele data for full details of the genetic interactions
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  ru
                  suppressible
                  rho::ru
                  (Yogev et al., 2010)
                  ru
                  enhanceable
                  rho
                  (Birkholz et al., 2009, Wasserman et al., 2000)
                  ru
                  suppressible
                  rho
                  (Gallio et al., 2004)
                  rho|ru
                  enhanceable
                  sens
                  (Frankfort and Mardon, 2004)
                  ru
                  suppressible
                  sl
                  (Schlesinger et al., 2004)
                  ru
                  suppressible
                  spi
                  (Yu et al., 2010)
                  ru
                  suppressible
                  Syx5
                  (Yogev et al., 2010)
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  caz
                  suppressible
                  ru
                  (Shimamura et al., 2014)
                  net
                  enhanceable
                  ru
                  (Nagel et al., 2016)
                  Rab11
                  suppressible
                  ru
                  (Yogev et al., 2010)
                  rho
                  enhanceable
                  ru
                  (Wasserman et al., 2000)
                  External Data
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  DroID - A comprehensive database of gene and protein interactions.
                  MIST (genetic) - An integrated Molecular Interaction Database
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Pathways
                  Signaling Pathways (FlyBase)
                  EGFR Ligand Production -
                  Factors required for the proteolytic cleavage and/or secretion of transforming growth factor-α-like Egfr ligands spi, Krn or grk.
                  Metabolic Pathways
                  External Data
                  Linkouts
                  SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                  Genomic Location and Detailed Mapping Data
                  Chromosome (arm)
                  3L
                  Recombination map
                  3-0
                  Cytogenetic map
                  61F8-61F8
                  Sequence location
                  FlyBase Computed Cytological Location
                  Cytogenetic map
                  Evidence for location
                  61F8-61F8
                  Limits computationally determined from genome sequence between P{PZ}l(3)0264002640 and P{EP}rhoEP3704&P{PZ}l(3)0622606226
                  Experimentally Determined Cytological Location
                  Cytogenetic map
                  Notes
                  References
                  61F8-61F8
                  (Freeman, 2000.11.2)
                  Experimentally Determined Recombination Data
                  Location

                  3-0

                  (FlyBase, 2016)

                  3-1.7

                  (Comeron et al., 2012)

                  3-0.0

                  (Fernandez Gianotti, 1952)
                  Left of (cM)
                  Right of (cM)
                  Notes

                  Maps 2.57 units to the right of the end of the chromosome, based on recombination with y+ of T(1;3)scJ4 in a sample of 9106 flies.

                  (Strommen and Falk, 1981)
                  Stocks and Reagents
                  Stocks (247)
                  Genomic Clones (24)
                  cDNA Clones (1)
                   

                  Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                  cDNA clones, fully sequenced
                  BDGP DGC clones
                  Other clones
                    Drosophila Genomics Resource Center cDNA clones

                    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                    cDNA Clones, End Sequenced (ESTs)
                    BDGP DGC clones
                      Other clones
                        RNAi and Array Information
                        Linkouts
                        DRSC - Results frm RNAi screens
                        Antibody Information
                        Laboratory Generated Antibodies
                         
                        Commercially Available Antibodies
                         
                        Cell Line Information
                        Publicly Available Cell Lines
                         
                          Other Stable Cell Lines
                           
                            Other Comments

                            ru protein cleaves S within its transmembrane domain.

                            (Tsruya et al., 2007)

                            ru, expressed in the ventral nerve cord, is necessary for repulsion of ganglionic tracheal branches from the ventral midline.

                            (Gallio et al., 2004)

                            ru regulates Egfr signalling in the eye.

                            (Wasserman et al., 2000)

                            The abnormal ru phenotype is due to a developmental defect: late occurring defects. Adult eyes rough and reduced, with photoreceptor and pigment cell defects, eye disc pattern appears normal.

                            (Renfranz and Benzer, 1989)
                            Relationship to Other Genes
                            Source for database merge of

                            Source for merge of: ru rhomboid-3 CG1214

                            (Wasserman et al., 2000)
                            Additional comments
                            Nomenclature History
                            Source for database identify of
                            Nomenclature comments
                            Etymology
                            Synonyms and Secondary IDs (17)
                            Reported As
                            Symbol Synonym
                            CG1214
                            (Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Corona et al., 2007, Molnar et al., 2006)
                            Rho-3
                            (Yogev et al., 2008, Yogev et al., 2007, Urban et al., 2002)
                            Rho-related [62A1]
                            (Guichard et al., 2000)
                            Rho3
                            (Martín-Bermudo et al., 2015)
                            Ru
                            (Yamaguchi et al., 2021)
                            rho-3
                            (Shwartz et al., 2013, Yogev et al., 2010, Yu et al., 2010, Jiang and Edgar, 2009, Yogev et al., 2008, Frankfort and Mardon, 2004, Wasserman et al., 2000)
                            rho3
                            (Rossi and Fernandes, 2018, Jiang et al., 2011, Yu et al., 2010, Tsruya et al., 2007, Frankfort et al., 2004, Gallio et al., 2004, Frankfort and Mardon, 2002)
                            ru
                            (Schlichting et al., 2022, Kumar et al., 2021, McLaughlin et al., 2021, Yamamoto et al., 2019, Duan et al., 2018, Kavaler et al., 2018, Newcomb et al., 2018, Ozasa et al., 2017, Malartre, 2016, Nagel et al., 2016, Dembeck et al., 2015, Sun et al., 2015, Shimamura et al., 2014, Shwartz et al., 2013, Steinhauer et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Nfonsam et al., 2012, Guan et al., 2011, Yogev et al., 2010, Yu et al., 2010, Birkholz et al., 2009, Jiang and Edgar, 2009, FlyBase Curators, 2008-, Brown et al., 2007, Corona et al., 2007, Brown et al., 2006, Charroux et al., 2006, Hashimoto and Yamaguchi, 2006, Molnar et al., 2006, Parker, 2006, Lipatov et al., 2005, Schlesinger et al., 2004, Takahashi and Ting, 2004)
                            Name Synonyms
                            Rhomboid 3
                            (Urban, 2001.10.16)
                            Rhomboid-3
                            (Tsruya et al., 2007)
                            Roughoid
                            (Steinhauer et al., 2013, Tsruya et al., 2007, Hashimoto and Yamaguchi, 2006)
                            rhomboid
                            (Shimamura et al., 2014)
                            rhomboid 3
                            (Zhu et al., 2017, Guan et al., 2011, Yu et al., 2010)
                            rhomboid-3
                            (Yamaguchi et al., 2021, Paschkowsky et al., 2019, Hashimoto and Yamaguchi, 2007, Hashimoto and Yamaguchi, 2006, Urban, 2006, Urban et al., 2004, Urban and Freeman, 2003)
                            rhomboid3
                            (Shilo, 2016, Martín-Bermudo et al., 2015)
                            roughoid
                            (Ozasa et al., 2017, Malartre, 2016, Boseman et al., 2013, Külshammer and Uhlirova, 2013, Guan et al., 2011, Baig et al., 2010, Yu et al., 2010, Birkholz et al., 2009, Takahashi and Ting, 2004)
                            roughoid/rhomboid-3
                            (Wasserman et al., 2000)
                            Secondary FlyBase IDs
                            • FBgn0035215
                            • FBgn0041724
                            Datasets (0)
                            Study focus (0)
                            Experimental Role
                            Project
                            Project Type
                            Title
                            Study result (0)
                            Result
                            Result Type
                            Title
                            External Crossreferences and Linkouts ( 36 )
                            Sequence Crossreferences
                            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                            Other crossreferences
                            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                            FlyMine - An integrated database for Drosophila genomics
                            InterPro - A database of protein families, domains and functional sites
                            KEGG Genes - Molecular building blocks of life in the genomic space.
                            MARRVEL_MODEL - MARRVEL (model organism gene)
                            Linkouts
                            BioGRID - A database of protein and genetic interactions.
                            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                            DroID - A comprehensive database of gene and protein interactions.
                            DRSC - Results frm RNAi screens
                            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                            Flygut - An atlas of the Drosophila adult midgut
                            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                            MIST (genetic) - An integrated Molecular Interaction Database
                            MIST (protein-protein) - An integrated Molecular Interaction Database
                            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                            References (153)