FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Taf6, Taf9
FlyBase ID
FBig0000098927
Interaction Network
Interactions Browser links
Taf6 network
Taf9 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0064477-2.coIP.WB
Reference
Goodrich et al., 1993
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf9 

dTAF[[II]]40

bait
Taf6 

dTAF[[II]]60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was dTAFIID-containing fraction derived from embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Goodrich et al., 1993)
FBrf0192616-14.coIP.WB
Reference
Wright et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Taf6 

TAF6

bait

FLAG tag

Taf6 
Taf9 

TAF9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf6 
NTR domain
sufficient binding region
aa 1-227
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.

(Wright et al., 2006)
FBrf0209543-106.PD.WB
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf9 

TAF9

bait

GST tag

Taf6 

TAF6

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Weake et al., 2009)

Sequential GST pull down and anti-FLAG coimmunoprecipitation of coexpressed proteins.

(Weake et al., 2009)
FBrf0209543-88.coIP.MS
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Taf6 

TAF6

bait

FLAG tag, HA tag

Taf9 

TAF9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Weake et al., 2009)

The Drosophila TFIID complex is composed of the following subunits: e(y)1, (Taf9), Taf10b, Taf12, Taf1, Taf2, bip2, Taf4, Taf5, Taf6, Taf7, Taf8, Taf10, Taf11, Taf13, Tbp, Trf and Trf2@.

(Weake et al., 2009)

In Drosophila, Taf6 is restricted to the TFIID complex and is not found in the Drosophila SAGA complex.

(Weake et al., 2009)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Weake et al., 2009)

Positive control.

(Weake et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)