Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast

stck, parvin, Ilk and ics copurified stoichiometrically.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

stck, parvin, Ilk and ics copurified stoichiometrically.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Pulldown performed under native conditions using an Ni-NTA agarose gel.

Interaction in vitro; bait produced as transgenic fusion protein; prey derived from S2 cell extract.

One of three stck interactions observed in two embryo replicates and in S2 cells.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

One of three stck interactions observed in two embryo replicates and in S2 cells.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.